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PDI monoclonal antibody (1D3)

ADI-SPA-891-D 50 µg 194.00 USD
ADI-SPA-891-F 200 µg 410.00 USD
ADI-SPA-891-J 1 mg 1,619.00 USD
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Product Specification

Alternative Name:P4HB, protein disulfide isomerase
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of rat PDI.
UniProt ID:P04785
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Applications:ICC, IF, IHC, WB
Electron microscopy
Recommended Dilutions/Conditions:Immunohistochemistry (1:200-1:500)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~58kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The mammalian PDI (Protein disulfide-isomerase) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the endoplasmic reticulum by catalyzing the rearrangement of disulfide bonds.
SPA-891 WB
Western blot analysis of PDI: Lane 1: MW marker, Lane 2: HeLa (HS) Lane 3: ADI-SPP-891, Lane 4: Vero (HS), Lane 5: 3T3 (HS), Lane 6: PC-12 (HS), Lane 7: CHO-K1 (HS)
Immunohistochemistry analysis of human HEK293 cells transfected with wild type TorsinA co-stained with PDI mAb (1D3) (red). Co-localization of TorsinA and PDI proteins merge as yellow.
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SPA-891 WB SPA-891 IHC

Product Literature References

COMP-assisted collagen secretion--a novel intracellular function required for fibrosis: J.N. Schulz, et al.; J. Cell Sci. 129, 706 (2016), Application(s): immunofluorescence staining of collagen I and collagen XII, Abstract; Full Text
Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia: N.A. Bolar, et al.; Am. J. Hum. Genet. 99, 174 (2016), Application(s): Intracellular localization of SEC61A1 in endoplasmic reticulum, Abstract;
Comparative proteomic analysis of compartmentalised Ras signalling: M. Hernandez-Valladares, et al. ; Sci. Rep. 5, 17307 (2015), Application(s): Colocalization studies, Abstract; Full Text
Hints to the diagnosis of uromodulin kidney disease: T. Onoe, et al.; Clin. Kidney J. (2015), Application(s): Immunostaining of Tamm-Horsfall protein, Full Text
Human Cytomegalovirus Up-Regulates Endothelin Receptor Type B: Implication for Vasculopathies?: K.C. Yaiw, et al. ; Open Forum Infect. Dis. 2, ofv155 (2015), Application(s): Immunofluorescence, Abstract; Full Text
ISG15 counteracts Listeria monocytogenes infection: L. Radoshevich, et al.; eLife 4, e06848 (2015), Application(s): Cell culture, Abstract;
Orotate phosphoribosyltransferase localizes to the Golgi complex and its expression levels affect the sensitivity to anti-cancer drug 5-fluorouracil: Y. Hozumi, et al. ; Biomed. Res. 36, 403 (2015), Application(s): Immunocytochemistry, Abstract; Full Text
Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum: E. Avezov, et al.; BMC Biol 13, 2 (2015), Application(s): Western Blotting, Abstract; Full Text
Sterol liganding of OSBP-related proteins (ORPs) regulates the subcellular distribution of ORP-VAPA complexes and their impacts on organelle structure: H. Kentala, et al.; Steroids 99, 248 (2015), Application(s): Staining, Abstract;
Antibodies That Detect O-Linked β-D-N-Acetylglucosamine on the Extracellular Domain of Cell Surface Glycoproteins: Y. Tashima, et al.; J. Biol. Chem. 289, 11132 (2014), Abstract;
Calreticulin from suboolemmal vesicles affects membrane regulation of polyspermy: M.D. Saavedra, et al.; Reproduction 147, 369 (2014), Abstract;
Neuregulin-1 is concentrated in the postsynaptic subsurface cistern of C-bouton inputs to a-motoneurons and altered during motoneuron diseases: X. Gallart-Palau, et al.; FASEB J. 28, 3618 (2014), Abstract;
Sensory neuropathy with bone destruction due to a mutation in the membrane-shaping atlastin GTPase 3: U. Kornak, et al.; Brain 137, 683 (2014), Abstract;
Deficiency of TDAG51 Protects Against Atherosclerosis by Modulating Apoptosis, Cholesterol Efflux, and Peroxiredoxin-1 Expression: G.S. Hossain, et al.; JAHA 2, e000134 (2013), Application(s): IHC using human carotid arteries, Abstract; Full Text
Endoplasmic Reticulum Stress and Glycogen Synthase Kinase-3β Activation in Apolipoprotein E–Deficient Mouse Models of Accelerated Atherosclerosis: C.S. McAlpine, et al.; Arterioscler. Thromb. Vasc. Biol. 32, 82 (2012), Application(s): WB using mouse liver lysates, Abstract; Full Text
Quantitative Proteomics Reveals That Only a Subset of the Endoplasmic Reticulum Contributes to the Phagosome: F.-X. Campbell-Valois, et al.; Mol. Cell. Proteomics 11, M111.016378 (2012), Application(s): WB using murine macrophage J774A.1 cells, Abstract; Full Text
Reduced Calreticulin Levels Link Endoplasmic Reticulum Stress and Fas-Triggered Cell Death in Motoneurons Vulnerable to ALS: N. Bernard-Marissal, et al.; J. Neurosci. 32, 4901 (2012), Application(s): ICC using murine motorneurons, Abstract; Full Text
Calreticulin chaperones regulate functional expression of vomeronasal type 2 pheromone receptors: S. Dey & H. Matsunami; PNAS 108, 16651 (2011), Application(s): ICC using murine NIH/3T3 cells, Abstract; Full Text
Hepatitis C Virus NS2 Protein Serves as a Scaffold for Virus Assembly by Interacting with both Structural and Nonstructural Proteins: Y. Ma, et al.; J. Virol. 85, 86 (2011), Application(s): ICC using human FT3-7 hepatoma cells, Abstract; Full Text
The Antifungal Drug Itraconazole Inhibits Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) Glycosylation, Trafficking, and Signaling in Endothelial Cells: B.A. Nacev, et al.; J. Biol. Chem. 286, 44045 (2011), Application(s): ICC using human vascular endothelial cells (HUVEC), Abstract; Full Text
The sarcoplasmic reticulum luminal thiol oxidase ERO1 regulates cardiomyocyte excitation-coupled calcium release and response to hemodynamic load: K.-T. Chin, et al.; FASEB J. 25, 2583 (2011), Application(s): WB using mouse embryonic fibroblasts (MEF), Abstract; Full Text
Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution: A. Chadrin, et al.; J. Cell Biol. 189, 795 (2010), Application(s): Fluorescence microscopy, Abstract;
A gradient of ROR2 protein stability and membrane localization confers brachydactyly type B or Robinow syndrome phenotypes: W. Schwarzer, et al.; Hum. Mol. Genet. 18, 4013 (2009), Application(s): ICC using human Cos-1/HEK293 FlpIn T-Rex cells, Abstract; Full Text
Airway Epithelial Inflammation-induced Endoplasmic Reticulum Ca(2+)Store Expansion Is Mediated by X-box Binding Protein-1: C. Ribeiro, et al. ; J. Biol. Chem. 284, 14904 (2009), Application(s): WB using human tissue, Abstract;
Loss of Specific Chaperones Involved in Membrane Glycoprotein Biosynthesis during the Maturation of Human Erythroid Progenitor Cells: S.T. Patterson, et al.; J. Biol. Chem. 284, 14547 (2009), Application(s): WB using primary human erythroids, Abstract; Full Text
Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death: T. Panaretakis, et al.; EMBO J. 28, 578 (2009), Application(s): WB using murine CT26 cells, Abstract; Full Text
Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages: C.X.C. Santos, et al.; J. Leukoc. Biol. 86, 989 (2009), Application(s): WB and ICC using murine macrophage-like J774 cells, Abstract; Full Text
Reticulon-4A (Nogo-A) Redistributes Protein Disulfide Isomerase to Protect Mice from SOD1-Dependent Amyotrophic Lateral Sclerosis: Y.S. Yang, et al.; J. Neurosci. 29, 13850 (2009), Application(s): IHC using murine lumbar spinal cord samples, Abstract; Full Text
A Novel Actin-Related Protein Is Associated with Daughter Cell Formation in Toxoplasma gondii: J.L. Gordon , et al.; Eukaryot. Cell 7, 1500 (2008), Application(s): EM using parasite-infected human foreskin fibroblasts (HFF), Abstract; Full Text
Characterization of RhoBTB-dependent Cul3 ubiquitin ligase complexes -Evidence for an autoregulatory mechanism: F. Rivero, et al. ; Exp. Cell Res. 314, 3453 (2008), Application(s): ICC, IF using monkey tissue culture, Abstract;
Heterotypic tubular connections at the endoplasmic reticulum-Golgi complex interface: J. Martinez-Menarguez, et al. ; Histochem. Cell Biol. 130, 709 (2008), Application(s): EM using rat tissue, Abstract;
Proteasomal adaptation to environmental stress links resistance to proteotoxicity with longevity in Caenorhabditis elegans: C. Yun, et al.; PNAS 105, 7094 (2008), Application(s): ICC using monkey COS1 cells, Abstract; Full Text
The AAA-ATPase p97 facilitates degradation of apolipoprotein B by the ubiquitin-proteasome pathway: E.A. Fisher, et al.; J. Lipid Res. 49, 2149 (2008), Application(s): WB using human HepG2 cells, Abstract; Full Text
TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton: C. Flávia, et al.; J. Cell Sci. 121, 3476 (2008), Application(s): ICC using mouse embryonic fibroblasts (MEF), Abstract; Full Text
Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis: D.B. Gould, et al.; Hum. Mol. Genet. 16, 798 (2007), Application(s): IHC using murine enucleated eyes, Abstract; Full Text
Disulfide-dependent protein folding is linked to operation of the vitamin K cycle in the endoplasmic reticulum. A protein disulfide isomerase-VKORC1 redox enzyme complex appears to be responsible for vitamin K1 2,3-epoxide reduction.: N. Wajih, et al.; J. Biol. Chem. 282, 2626 (2007), Application(s): WB using transfected hamster BHK-21 cells, Abstract; Full Text
Missense Mutations in APOB within the beta{alpha}1 Domain of Human APOB-100 Result in Impaired Secretion of ApoB and ApoB-containing Lipoproteins in Familial Hypobetalipoproteinemia: J.R. Burnett, et al.; J. Biol. Chem. 282, 24270 (2007), Application(s): WB using transfected rat McA-RH7777 cells, Abstract; Full Text
Mutant torsinA interferes with protein processing through the secretory pathway in DYT1 dystonia cells: J.W. Hewett, et al.; PNAS 104, 7271 (2007), Application(s): ICC using human fibroblast cell lines, Abstract; Full Text
Thromboxane hypersensitivity in hypoxic pulmonary artery myocytes: altered TP receptor localization and kinetics: S. Dakshinamurti, et al. ; Am. J. Physiol. Lung Cell Mol. Physiol. 292, L654 (2007), Application(s): ICC using porcine tissue culture, Abstract;
Changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding {alpha}1-antitrypsin transgenic mice: E. Papp, et al.; FASEB J. 20, 1018 (2006), Application(s): IP using murine liver microsomes, Abstract; Full Text
Functions of ERp57 in the Folding and Assembly of Major Histocompatibility Complex Class I Molecules: Y. Zhang, et al.; J. Biol. Chem. 281, 14622 (2006), Application(s): FC using mouse L cells, Abstract; Full Text
The cellular response to heat stress in the goby Gillichthys mirabilis: a cDNA microarray and protein-level analysis: B.A. Buckley, et al.; J. Exp. Biol. 209, 2660 (2006), Application(s): WB using fish (cooper) gill and white muscle tissue samples, Abstract; Full Text
Contribution of Cubilin and Amnionless to Processing and Membrane Targeting of Cubilin–Amnionless Complex: G. Coudroy, et al.; J. Am. Soc. Nephrol. 16, 2330 (2005), Application(s): ICC using canine MDCK cells, Abstract; Full Text
Different MHC Class I Heavy Chains Compete with Each Other for Folding Independently of β2-Microglobulin and Peptide: S. Tourdot, et al.; J. Immunol. 174, 925 (2005), Application(s): ICC using transfected hamster CHO cells, Abstract; Full Text
Hydrolethalus syndrome is caused by a missense mutation in a novel gene HYLS1: L. Mee, et al.; Hum. Mol. Genet. 14, 1475 (2005), Application(s): ICC using transfected monkey COS-1 cells, Abstract; Full Text
Tapasin and ERp57 form a stable disulfide-linked dimer within the MHC class I peptide-loading complex: D.R. Peaper, et al.; EMBO J. 24, 3613 (2005), Application(s): IP using human HeLa-M, FO-1, FO-1.b2m and CEM.NKR cells, Abstract; Full Text
Misfolding of Major Histocompatibility Complex Class I Molecules in Activated T Cells Allows cis-Interactions with Receptors and Signaling Molecules and Is Associated with Tyrosine Phosphorylation: S.G. Santos, et al.; J. Biol. Chem. 279, 53062 (2004), Application(s): WB using human peripheral blood T lymphocytes (PBL), Abstract; Full Text
Altered localization of Drosophila Smoothened protein activates Hedgehog signal transduction: A.J. Zhu, et al.; Genes & Dev. 17, 1240 (2003), Application(s): ICC using Drosophila salivary gland cells, Abstract; Full Text
Chaperone action in the posttranslational topological reorientation of the hepatitis B virus large envelope protein: Implications for translocational regulation: C. Lambert & R. Prange; PNAS 100, 5199 (2003), Application(s): WB using transfected, immunoprecipitated monkey COS-7 cells, Abstract; Full Text
Determinant for Endoplasmic Reticulum Retention in the Luminal Domain of the Human Cytomegalovirus US3 Glycoprotein: S. Lee, et al.; J. Virol. 77, 2147 (2003), Application(s): ICC using human HtTa cells, Abstract; Full Text
Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles: M. Hsu , et al.; PNAS 100, 7271 (2003), Application(s): FC using human transfected 293-T cells, Abstract; Full Text
Activation of myosin V-based motility and F-actin-dependent network formation of endoplasmic reticulum during mitosis: S.A. Kuznetsov, et al. ; J. Cell Biol. 159, 571 (2002), Application(s): ICC using xenopus samples, Abstract;
Differential interaction of molecular chaperones with procollagen I and type IV collagen in corneal endothelial cells: E.P. Kay, et al. ; Mol. Vis. 8, 9 (2002), Application(s): WB using rabbit samples, Abstract;
TorsinA immunoreactivity in brains of patients with DYT1 and non-DYT1 dystonia: P. Shashidharan, et al. ; Neurology 58, 120 (2002), Application(s): IHC using human samples, Abstract;
Kinectin is a key effector of RhoG microtubule-dependent cellular activity: P. Fort, et al. ; Mol. Cell. Biol. 21, 8022 (2001), Application(s): ICC using monkey & rat samples, Abstract;
Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man: T.J. Jentsch, et al. ; Cell 104, 205 (2001), Application(s): IHC using mouse samples, Abstract;
A 56-kDa Selenium-binding Protein Participates in Intra-Golgi Protein Transport: A. Porat, et al.; J. Biol. Chem. 275, 14457 (2000), Application(s): WB using rat liver lysate fractions, Abstract; Full Text
Mutant torsinA, responsible for early-onset torsion dystonia, forms membrane inclusions in cultured neural cells: J. Hewett, et al.; Hum. Mol. Genet. 9, 1403 (2000), Application(s): ICC using mouse neural CAD cells, Abstract; Full Text
Chromatin degradation in differentiating fiber cells of the eye lens: D. Mataic, et al. ; J. Cell Biol. 137, 37 (1997), Application(s): IHC using chicken samples, Abstract;

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