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PDI polyclonal antibody

 
ADI-SPA-890-D 50 µg 161.00 USD
 
ADI-SPA-890-F 200 µg 356.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:P4HB, protein disulfide isomerase
 
Host:Rabbit
 
Immunogen:Purified bovine liver Protein Disulfide Isomerase.
 
UniProt ID:P05307
 
GenBank ID:M17596
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Sheep, Xenopus
 
Applications:ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~58kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The mammalian PDI (Protein disulfide-isomerase) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the endoplasmic reticulum by catalyzing the rearrangement of disulfide bonds.
 
SPA-890 IHC
Immunohistochemistry analysis of human spleen tissue stained with PDI, pAb at 10µg/ml.
SPA-890 WB 01
Western Blot Analysis of PDI: Lane 1: MWM, Lane 2: PDI (human), (recombinant) (His-tag) (Prod. No.ADI-SPP-891), Lane 3: HeLa, (cell lysate) (Prod. No.ADI-LYC-HL100), Lane 4: Rat Testis Tissue Lysate, Lane 5: Mouse Spleen Tissue Lysate.
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SPA-890 IHC SPA-890 WB 01

Product Literature References

Jagn1 Is Induced in Response to ER Stress and Regulates Proinsulin Biosynthesis: C. Nosak, et al.; PLoS One 11, e0149177 (2016), Application(s): Immunofluorescence microscopy, Abstract; Full Text
Characterization and Mechanism of Stress-induced Translocation of 78-kilodalton Glucose Regulated Protein (GRP78) to the Cell Surface: Y.L. Tsai, et al.; J. Biol. Chem. 290, 8049 (2015), Application(s): Western Blotting, Abstract; Full Text
Insufficient glucose supply is linked to hypothermia upon cold exposure in mice lacking phosphatidylethanolamine N-methyltransferase: X. Gao, et al.; J. Lipid. Res. 56, 1701 (2015), Application(s): Western Blot, Abstract; Full Text
On the Role of Protein Disulfide Isomerase in the Retrograde Cell Transport of Secreted Phospholipases A2: J. Oberčkal, et al.; PLoS One 10, e0120692 (2015), Application(s): Immunoprecipitation , Abstract; Full Text
Impaired ERAD and ER stress are early and specific events in polyglutamine toxicity: S. Lindquist, et al. ; Genes Dev. 22, 3308 (2008), Application(s): WB using human cell lysates, Abstract;
Membrane topography of human phosphatidylethanolamine N-methyltransferase: D.E. Vance, et al. ; J. Biol. Chem. 278, 2956 (2003), Application(s): WB using monkey samples, Abstract;
Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice: J. Dean, et al. ; Mol. Cell. Biol. 22, 3111 (2002), Application(s): ICC using mouse samples, Abstract;
Maturation of lipoprotein lipase in the endoplasmic reticulum: concurrent formation of functional dimers and inactive aggregates: M.H. Doolittle, et al. ; J. Biol. Chem. 277, 10727 (2002), Application(s): IP using human samples, Abstract;
Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells: G. Warren, et al. ; Science 295, 848 (2002), Application(s): WB using rat samples, Abstract;
Trafficking of lysosomal cathepsin B-green fluorescent protein to the surface of thyroid epithelial cells involves the endosomal/lysosomal compartment: K. Brix, et al. ; J. Cell. Sci. 115, 4877 (2002), Application(s): ICC using rat samples, Abstract;
Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells: A.H. Futerman, et al. ; J. Biol. Chem. 277, 35642 (2002), Application(s): ICC using monkey samples, Abstract;
Protein-disulfide isomerase (PDI) in FRTL5 cells - pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes: R. Miquelis, et al. ; J. Biol. Chem. 275, 1920 (2000), Application(s): IP using rat samples, Abstract;
Specificity in substrate binding by protein folding catalysts: tyrosine and tryptophan residues are the recognition motifs for the binding of peptides to the pancreas-specific protein disulfide isomerase PDIp: P. Klappa, et al. ; Protein Sci. 9, 758 (2000), Application(s): WB using sheep samples, Abstract;
Type I alpha phosphatidylinositol 4-phosphate 5-kinase is a putative target for increased intracellular phosphatidic acid: I. Merida, et al. ; FEBS Lett. 476, 160 (2000), Application(s): ICC using porcine samples, Abstract;

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