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HSP65 (mycobacterial) monoclonal antibody (3F7)

 
ADI-SPA-881-D 50 µl 194.00 USD
 
ADI-SPA-881-F 200 µl 399.00 USD
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Product Specification

Alternative Name:Heat shock protein 65
 
Clone:3F7
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Recombinant M. bovis HSP65.
 
UniProt ID:P0A521
 
Species reactivity:Human, Mouse, Rat
Bovine, Cryptosporidium, Drosophila, E. coli, Guinea pig, Hamster, Mycobacterium bovis, Mycobacterium leprae
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~65kDa by Western blot.
 
Purity Detail:Ammonium sulfate precipitation purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Hsp65 is a member of the Hsp60 family of heat shock proteins isolated from Mycobacterium bovis BCG. Hsp65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. Hsp65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats.
 
SPA-881 WB
Western blot analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E. coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.
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SPA-881 WB

Product Literature References

gamma delta+ T cells and 65-kDa heat shock protein expression following Cryptosporidium parvum challenge in athymic C57BL/6J nude mice: F.J. Enriquez, et al. ; Immunol. Lett. 72, 35 (2000), Application(s): WB using cryptosporidium samples, Abstract;

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
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