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DnaK (E. coli) monoclonal antibody (8E2/2)

ADI-SPA-880-D 50 µg 198.00 USD
ADI-SPA-880-F 200 µg 418.00 USD
ADI-SPA-880-J 1 mg 1,629.00 USD
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Product Specification

Alternative Name:Hsp70
Immunogen:E. coli DnaK.
UniProt ID:P0A6Y8 (strain K12)
GenBank ID:AP009048
Species reactivity:E. coli
Recommended Dilutions/Conditions:Western Blot (1:10,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~70kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
SPA-880 WB
Western blot analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein (Prod. No. ADI-SPP-630), Lane 3: E. coli cell lysate.
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SPA-880 WB

Product Literature References

Comparison of Salmonella enterica serovars Typhi and Typhimurium reveals typhoidal-specific responses to bile: R. Johnson, et al.; Infect. Immun. 86, e00490 (2018), Abstract; Full Text
Molecular basis for CesT recognition of type III secretion effectors in enteropathogenic Escherichia coli: D. J. Little, et al.; Plos Pathog. 14, e1007224 (2018), Abstract; Full Text
Identification of New Virulence Factors and Vaccine Candidates for Yersinia pestis: J.A. Andersson, et al.; Front. Cell. Infect. Microbiol. 7, 448 (2017), Abstract; Full Text
Influence of Escherichia coli chaperone DnaK on protein immunogenicity: R.D. Ratanji, et al.; Immunology 150, 343 (2017), Abstract; Full Text
Reductive evolution in outer membrane protein biogenesis has not compromised cell surface complexity in Helicobacter pylori: C.T. Webb, et al.; Microbiologyopen 6, e00513 (2017), Abstract; Full Text
The malS-5’UTR regulates hisG, a keygene in the histidine biosynthetic pathway in Salmonella enterica serovar Typhi: Y. Zhang, et al.; Can. J. Microbiol. 63, 287 (2017), Abstract;
The type III secretion system effector SptP of Salmonella enterica serovar Typhi: R. Johnson, et al.; J. Bacteriol. 199, e00647 (2017), Abstract; Full Text
Heterologous Expression of Thermolabile Proteins Enhances Thermotolerance in Escherichia coli: Y. Ueda, et al.; Adv. Microbiol. 6, 602 (2016), Application(s): Western blot analysis, Full Text
Missing the target: DNAk is a dominant epitope in the humoral immune response of channel catfish (Ictalurus punctatus) to Flavobacterium columnare: M.D. Lange, et al.; Fish Shellfish Immunol. 51, 170 (2016), Application(s): 1-D gel electrophoresis and western blot analyses, Abstract;
Activity of a Bacterial Cell Envelope Stress Response is Controlled by the Interaction of a Protein-binding Domain with Different Partners: J. Flores-Kim, et al.; J. Biol. Chem. 290, 11417 (2015), Application(s): Western Blot, Abstract; Full Text
CCR2+ Inflammatory Dendritic Cells and Translocation of Antigen by Type III Secretion Are Required for the Exceptionally Large CD8+ T Cell Response to the Protective YopE69-77 Epitope during Yersinia Infection: Y. Zhang, et al.; PLoS Pathog. 11, e1005167 (2015), Application(s): Immunoblotting, Abstract; Full Text
Further characterization of a highly attenuated Yersinia pestis CO92 mutant deleted for the genes encoding Braun lipoprotein and plasminogen activator protease in murine alveolar and primary human macrophages: C.J. van Lier, et al.; Microb. Pathog. 80, 27 (2015), Application(s): Western Blotting, Abstract;
Influence of gamma irradiation on expressed heat shock proteins of Pantoea agglomerans, a yellow pigmented phytopathogen: D. Dussault, et al.; Microbiol. Res. Int. 3, 51 (2015), Application(s): Western Blot, Full Text
Novel HSP90 inhibitors effectively target functions of thyroid cancer stem cell preventing migration and invasion: P.T. White, et al.; Surgery 159, 142 (2015), Application(s): Western blot, Abstract;
Overexpression, Purification and Characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) Protein: T. Zininga, et al.; PLoS One 10, e0129445 (2015), Application(s): Western Blot, Abstract; Full Text
Shigella flexneri Regulation of ARF6 Activation during Bacterial Entry via an IpgD-Mediated Positive Feedback Loop: A.C. Garza-Mayers, et al.; MBio. 6, e02584-14 (2015), Application(s): SDS-PAGE, Abstract; Full Text
Scope and limitations of the designer proline-rich antibacterial peptide dimer, A3-APO, alone or in synergy with conventional antibiotics: J.r. Otvos, et al. ; Peptides 29, 1878 (2008), Application(s): WB using bacteria purified protein, Abstract;
TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis: S. Michalek, et al. ; J. Leukoc. Biol. 84, 1434 (2008), Application(s): WB using bacteria cell lysates, Abstract;
An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway: M. DeLisa, et al. ; J. Mol. Biol. 367, 715 (2007), Application(s): WB using bacteria cell lysates, Abstract;
Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator σ(S) upon carbon starvation: T. Nystroem, et al. ; Genes Dev. 21, 862 (2007), Application(s): WB using bacteria cell lysates, Abstract;
Escherichia coli heat shock protein DnaK: production and consequences in terms of monitoring cooking: L. Saucier, et al. ; Appl. Environ. Microbiol. 69, 3231 (2003), Application(s): EIA using bacteria (E. coli) samples, Abstract;
Secretin of the enteropathogenic Escherichia coli type III secretion system requires components of the type III apparatus for assembly and localization: B.B. Finlay, et al. ; Infect. Immun. 71, 3310 (2003), Application(s): WB using bacteria (E. coli) samples, Abstract;
Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems: B.B. Finlay, et al. ; Mol. Microbiol. 43, 1089 (2002), Application(s): WB using bacteria (Salmonella) samples, Abstract;
Adaptive and cross-protective responses of Pseudomonas sp. DJ-12 to several aromatics and other stress shocks: C.K. Kim, et al. ; Curr. Microbiol. 43, 176 (2001), Application(s): WB using bacteria (porcineseudomonas) samples, Abstract;
Detection of stress proteins in Porphyromonas gingivalis and other oral bacteria by Western immunoblotting analysis: D. Grenier, et al. ; FEMS Microbiol. Lett. 121, 303 (1994), Application(s): WB using bacteria (Actinomyces, Capnocytophaga sp.) samples, Abstract;

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