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Calnexin polyclonal antibody

 
ADI-SPA-865-D 50 µg 151.00 USD
 
ADI-SPA-865-F 200 µg 356.00 USD
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Product Specification

Alternative Name:CNX
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of dog calnexin.
 
UniProt ID:P24643
 
GenBank ID:X53616
 
Species reactivity:Human, Mouse, Rat
Bovine, C. elegans, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
 
Applications:ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~90kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.
 
SPA-865 WB
Western blot analysis: Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.
SPA-865 IHC
Immunohistochemistry analysis of human placenta tissue stained with Calnexin, pAb at 5µg/ml.
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SPA-865 WB SPA-865 IHC

Product Literature References

CLN5 is cleaved by members of the SPP/SPPL family to produce a mature soluble protein: F. Jules, et al.; Exp. Cell. Res. 357, 40 (2017), Abstract;
Fenofibrate, but not ezetimibe, prevents fatty liver disease in mice lacking phosphatidylethanolamine N-methyltransferase: J.N. van der Veen, et al.; J. Lipid. Res. 58, 656 (2017), Application(s): Western blot, Abstract; Full Text
Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission: P. Leblanc, et al.; Methods Mol. Biol. 1545, 153 (2017), Abstract;
Cannabinoid receptor subtype 2 (CB2R) agonist, GW405833 reduces agonist-induced Ca2+ oscillations in mouse pancreatic acinar cells: Z. Huang, et al.; Sci. Rep. 6, 29757 (2016), Application(s): CB2R Immunoblot Assay, Abstract; Full Text
Epigenetic Regulation of Steroid Inactivating UDP-Glucuronosyltransferases by microRNAs in Prostate Cancer: G. Margaillan, et al.; J. Steroid Biochem. Mol. Biol. 155, 85 (2016), Application(s): Protein immunodetection, Abstract;
Human Cytomegalovirus gH/gL Forms a Stable Complex with the Fusion Protein gB in Virions: A.L. Vanarsdall, et al.; PLoS Pathog. 12, e1005564 (2016), Application(s): Western Blot, Abstract; Full Text
Mannosidase IA is in Quality Control Vesicles and Participates in Glycoprotein Targeting to ERAD: N. Ogen-Shtern, et al.; J. Mol. Biol. 428, 3194 (2016), Application(s): Immunoblotting, Abstract;
Probing functional changes in exocyst configuration with monoclonal antibodies: S.M. Inamdar, et al.; Front. Cell. Dev. Biol. 4, 51 (2016), Application(s): Immunofluorescence, Abstract; Full Text
Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions: M.J. Gerl, et al.; PLoS One 11, e0153009 (2016), Application(s): Loading control, Abstract; Full Text
A Japanese encephalitis virus genotype 5 molecular clone is highly neuropathogenic in a mouse model: impact of the structural protein region on virulence: M. de Wispelaere, et al.; J. Virol. 89, 5862 (2015), Application(s): Western blot, Abstract; Full Text
A single amino acid substitution in the M protein attenuates Japanese Encephalitis virus in mammalian hosts: M. de Wispelaere, et al.; J. Virol. 90, 2676 (2015), Application(s): Western blot, Abstract; Full Text
A STIM2 splice variant negatively regulates store-operated calcium entry: A. M. Miederer, et al.; Nat. Commun. 6, 6899 (2015), Abstract; Full Text
Development-dependent regulation of molecular chaperones after hypoxia-ischemia: X. Sun, et al.; Neurobiol. Dis. 82, 123 (2015), Abstract;
Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics: S.J. Park, et al.; Sci. Rep. 5, 8694 (2015), Application(s): Immunogold Electron Microscopy, Abstract; Full Text
Exosomes, endogenous retroviruses and toll-like receptors: pregnancy recognition in ewes: I. Ruiz-Gonzalez, et al.; Reproduction 149, 281 (2015), Application(s): Western blot, Abstract; Full Text
Insufficient glucose supply is linked to hypothermia upon cold exposure in mice lacking phosphatidylethanolamine N-methyltransferase: X. Gao, et al.; J. Lipid. Res. 56, 1701 (2015), Application(s): Western Blot, Abstract; Full Text
Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma: D.L. Stankowska, et al.; Invest. Ophthalmol. Vis. Sci. 56, 893 (2015), Application(s): Western Blotting, Abstract; Full Text
Reduced Fertility and Altered Epididymal and Sperm Integrity in Mice Lacking ADAM7: H. Choi, et al.; Biol. Reprod. 93, 70 (2015), Application(s): Western Blot, Abstract; Full Text
Role for Rab10 in Methamphetamine-Induced Behavior: S.M. Vanderwerf, et al.; PLoS One 10, e0136167 (2015), Application(s): Western blot, Abstract; Full Text
Targeting membrane proteins to inner segments of vertebrate photoreceptors: Y. Pan; (2015), (PhD thesis), Application(s): Immunostaining, Abstract; Full Text
UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum: D. Lee, et al.; J. Biol. Chem. 290, 5725 (2015), Application(s): Western Blotting, Abstract; Full Text
Transcriptional regulation of apolipoprotein A-IV by the transcription factor CREBH: X. Xu, et al.; J. Lipid. Res. 55, 850 (2014), Application(s): Western blot, Abstract; Full Text
Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment: D. Paul, et al.; J. Virol. 87, 10612 (2013), Application(s): Immunofluorescence and Western blot, Abstract; Full Text
Catalytic properties of MGAT3, a putative triacylgycerol synthase: Y. Shi, et al. ; J. Lipid Res. 48, 583 (2007), Application(s): ICC using monkey tissue culture, Abstract;
Caenorhabditis elegans reticulon interacts with RME-1 during embryogenesis: T. Toyoda, et al. ; Biochem. Biophys. Res. Commun. 293, 698 (2002), Application(s): WB using C. elegans samples, Abstract;
Luman, the cellular counterpart of herpes simplex virus VP16, is processed by regulated intramembrane proteolysis: V. Misra, et al. ; Mol. Cell. Biol. 22, 5639 (2002), Application(s): WB using monkey samples, Abstract;
The glycoprotein gp48 of murine cytomegalovirus: proteasome-dependent cytosolic dislocation and degradation: U.H. Koszinowski, et al. ; J. Biol. Chem. 277, 2216 (2002), Application(s): ICC using mouse samples, Abstract;
Herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress: T. Miyata, et al. ; J. Biol. Chem. 275, 32846 (2000), Application(s): WB using human samples, Abstract;
Protein-disulfide isomerase (PDI) in FRTL5 cells - pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes: R. Miquelis, et al. ; J. Biol. Chem. 275, 1920 (2000), Application(s): ICC, WB using rat samples, Abstract;
Calnexin and the immunoglobulin binding protein (BiP) coimmunoprecipitate with AMPA receptors: R.J. Wenthold, et al. ; J. Neurochem. 73, 942 (1999), Application(s): IP using rat samples, Abstract;

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