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Calnexin polyclonal antibody

 
ADI-SPA-860-D 50 µg 211.00 USD
 
ADI-SPA-860-F 200 µg 398.00 USD
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Product Specification

Alternative Name:CNX
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of dog calnexin. The sequence is completely conserved in human, mouse and rat.
 
UniProt ID:P24643
 
GenBank ID:X53616
 
Source:Purified from rabbit serum.
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Quail, Rabbit, Sheep, Xenopus
 
Applications:ICC, IF, IHC (PS), IP, WB
Electron microscopy
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~90kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.
 
Calnexin polyclonal antibody Western blot
Western blot analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).
Calnexin polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human spleen tissue stained with Calnexin, pAb at 10µg/ml.
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Calnexin polyclonal antibody Western blot Calnexin polyclonal antibody Immunohistochemistry

Product Literature References

Neurite atrophy and apoptosis mediated by PERK signaling after accumulation of GM2-ganglioside: M.J. Virgolini, et al.; Biochim. Biophys. Acta Mol. Cell Res. 1866, 225 (2019), Abstract;
Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane: M. Blenski, et al.; Int. J. Mol. Sci. 20, 334 (2019), Abstract; Full Text
Zinc regulates ERp44-dependent protein quality control in the early secretory pathway: S. Watanabe, et al.; Nat. Commun. 10, 603 (2019), Application(s): IF, Abstract; Full Text
A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS: C. Lehmer, et al.; EMBO Mol. Med. 10, e8558 (2018), Abstract; Full Text
A selective ER‐phagy exerts procollagen quality control via a Calnexin‐FAM134B complex: A. Forrester, et al.; EMBO J. 2018, e99847 (2018), Abstract;
AP-SWATH Reveals Direct Involvement of VCP/p97 in Integrated Stress Response Signaling Through Facilitating CReP/PPP1R15B Degradation: J. Hulsmann, et al.; Mol. Cell. Proteomics 17, 1295 (2018), Abstract;
BACE1 inhibition more effectively suppresses initiation than progression of β-amyloid pathology: F. Peters, et al.; Acta Neuropathol. 135, 695 (2018), Abstract; Full Text
Cyclosporine A binding to COX-2 reveals a novel signaling pathway that activates the IRE1α unfolded protein response sensor: J. Groenendyk, et al.; Sci. Rep. 8, 16678 (2018), Abstract; Full Text
Exosome isolation from distinct biofluids using precipitation and column-based approaches: T. Soares Martins, et al.; PLoS One 13, e0198820 (2018), Abstract; Full Text
HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease: V. Benoy, et al.; Brain 141, 673 (2018), Abstract; Full Text
High-Throughput Screening Identifies Kinase Inhibitors That Increase Dual Adeno-Associated Viral Vector Transduction In Vitro and in Mouse Retina: A. Maddalena, et al.; Human Gene Ther. 29, 886 (2018), Abstract; Full Text
In Situ Structure of Neuronal C9orf72 Poly-GA Aggregates Reveals Proteasome Recruitment: Q. Guo, et al.; Cell 172, 696 (2018), Abstract; Full Text
Intra-prelimbic cortical inhibition of striatal-enriched tyrosine phosphatase suppresses cocaine seeking in rats: B.M. Siemsen, et al.; Addict. Biol. 23, 219 (2018), Abstract; Full Text
Loss of kallikrein‐related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice: K. Kidana, et al.; EMBO Mol. Med. 2018, e8184 (2018), Abstract; Full Text
Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation: A. Neerincx, et al.; Mol. Immunol. (2018), Abstract;
Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells: C.B.K. Jayson, et al.; Mol. Biol. Cell 29, 2045 (2018), Abstract; Full Text
Riluzole Impairs Cocaine Reinstatement and Restores Adaptations in Intrinsic Excitability and GLT-1 Expression: M.T. Sepulvedo-Orengo, et al.; Neuropsychopharmacology 43, 1212 (2018), Abstract;
The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells: Y. Radif, et al.; Mol. Cell. Biochem. 448, 275 (2018), Abstract; Full Text
The extended cytoplasmic tail of the human B4GALNT2 is critical for its Golgi targeting and post-Golgi sorting: S. Groux-Degroote, et al.; FEBS J. 285, 3442 (2018), Abstract;
The immunoglobulin-like domain of neuregulins potentiates ErbB3/HER3 activation and cellular proliferation: A. Centa, et al.; Mol. Oncol. 12, 1061 (2018), Abstract; Full Text
The Molecular Basis of Chemical Chaperone Therapy for Oculocutaneous Albinism Type 1A: A. Teramae, et al.; J. Invest. Dermatol. (2018), Abstract;
The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution: T. Sobajima, et al.; J. Cell Biol. 217, 1777 (2018), Abstract; Full Text
Xenopus ADAM19 regulates Wnt signaling and neural crest specification by stabilizing ADAM13: J. Li, et al.; Development 4, 145 (2018), Abstract;
A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication: C.N. Miller, et al.; Cell Host Microbe 22, 317 (2017), Abstract; Full Text
BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221): B. Dombert, et al.; Front. Mol. Neurosci. 10, 346 (2017), Abstract; Full Text
Beta-agonist stimulation ameliorates the phenotype of spinal and bulbar muscular atrophy mice and patient-derived myotubes: C. Milioto, et al.; Sci. Rep. 7, 41046 (2017), Abstract; Full Text
Dissecting the Process of Activation of Cancer-promoting Zinc-requiring Ectoenzymes by Zinc Metalation Mediated by ZNT Transporters: T. Tsuji, et al.; J. Biol. Chem. 292, 2159 (2017), Abstract; Full Text
Graded Elevation of c-Jun in Schwann Cells In Vivo: Gene Dosage Determines Effects on Development, Remyelination, Tumorigenesis, and Hypomyelination: S.V. Fazal, et al.; J. Neurosci. 37, 12297 (2017), Abstract; Full Text
Inhibition of IκBβ/NFκB signaling prevents LPS-induced IL1β expression without increasing apoptosis in the developing mouse lung: S. McKenna, et al.; Pediatr. Res. 82, 1064 (2017), Abstract; Full Text
Isolated polycystic liver disease genes define effectors of polycystin-1 function: W. Besse, et al.; J. Clin. Invest. 127, 1772 (2017), Abstract; Full Text
KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine: M. Puhka, et al.; Eur. J. Pharm. Sci. 98, 30 (2017), Application(s): Western blot, urine EVs, Abstract;
Legionella Effector AnkX Disrupts Host Cell Endocytic Recycling in a Phosphocholination-Dependent Manner: S.C. Allgood, et al.; Front. Cell. Infect. Microbiol. 7, 297 (2017), Abstract; Full Text
Model Based Targeting of IL-6-Induced Inflammatory Responses in Cultured Primary Hepatocytes to Improve Application of the JAK Inhibitor Ruxolitinib: S. Sobotta, et al.; Front. Physiol. 8, 775 (2017), Abstract; Full Text
Newly born peroxisomes are a hybrid of mitochondrial and ER-derived pre-peroxisomes: A. Sugiura, et al.; Nature 542, 251 (2017), Abstract;
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease: P. Luningschror, et al.; Nat. Commun. 8, 678 (2017), Abstract; Full Text
Protein kinase Cɛ activity regulates mGluR5 surface expression in the rat nucleus accumbens: M. Schwendt, et al.; J. Neurosci. Res. 95, 1079 (2017), Abstract; Full Text
Sex and Adolescent Ethanol Exposure Influence Pavlovian Conditioned Approach: A.C. Madayag, et al.; Alcohol Clin. Exp. Res. 41, 846 (2017), Abstract; Full Text
Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models: M. Yu, et al.; Cell Res. 27, 1441 (2017), Abstract; Full Text
THADA Regulates the Organismal Balance between Energy Storage and Heat Production: A. Moraru, et al.; Dev. Cell 41, 72 (2017), Abstract; Full Text
Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system: L. Ulbrich, et al.; J. Biochem. 473, 423 (2016), Abstract; Full Text
Calnexin, an ER-induced protein, is a prognostic marker and potential therapeutic target in colorectal cancer: D. Ryan, et al.; J. Transl. Med. 14, 196 (2016), Abstract; Full Text
Changes in the detergent-insoluble brain proteome linked to amyloid and tau in Alzheimer's Disease progression: C.M. Hales, et al.; Proteomics 16, 3042 (2016), Abstract; Full Text
Direct Comparison of Manganese Detoxification/Efflux Proteins and Molecular Characterization of ZnT10 Protein as a Manganese Transporter: Y. Nishito, et al.; J. Biol. Chem. 291, 14773 (2016), Abstract; Full Text
Generation and functional characterization of epithelial cells with stable expression of SLC26A9 Cl− channels: J. Salomon, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 310, L593 (2016), Abstract; Full Text
Glutamatergic neurotransmission in the prefrontal cortex mediates the suppressive effect of intra-prelimbic cortical infusion of BDNF on cocaine-seeking: B.S. Go, et al.; Eur. Neuropsychopharmacol. 26, 1989 (2016), Abstract; Full Text
Grp94 Delivers γ-aminobutyric Acid Type A (GABAA) Receptors to Hrd1-Mediatd Endoplasmic Reticulum-Associated Degradation: X.J. Di, et al.; J. Biol. Chem. 291, 9526 (2016), Application(s): Western blot, Abstract; Full Text
Helper-dependent adenoviral vectors for liver-directed gene therapy of primary hyperoxaluria type 1: R. Castello, et al.; Gene Ther. 23, 129 (2016), Abstract; Full Text
Homer2 regulates alcohol and stress cross-sensitization: S.G. Quadir, et al.; Addict. Biol. 21, 613 (2016), Application(s): Immunoblotting, Abstract;
Identification of VPS13C as a Galectin-12-Binding Protein That Regulates Galectin-12 Protein Stability and Adipogenesis: R.Y. Yang, et al.; PLoS One 11, e0153534 (2016), Application(s): HeLa cells immunostained with calnexin antibody, Abstract; Full Text
Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons: D. Schmitt, et al.; Histochem. Cell Biol. 146, 489 (2016), Application(s): Western blot, rabbit antiserum antibody, Abstract;
Integrative analysis identifies targetable CREB1/FoxA1 transcriptional co-regulation as a predictor of prostate cancer recurrence: B. Sunkel, et al.; Nucleic Acids Res. 44, 4105 (2016), Abstract; Full Text
Perinatal Endotoxemia Induces Sustained Hepatic COX-2 Expression through an NFκB-Dependent Mechanism: S. McKenna, et al.; J. Innate Immun. 8, 386 (2016), Abstract; Full Text
Proteolytic processing of Neuregulin 1 type III by three intramembrane cleaving proteases: D. Fleck, et al.; J. Biol. Chem. 291, 318 (2016), Application(s): Loading control, Abstract; Full Text
Quantitative Profiling of the Endonuclear Glycero-Phospholipidome of Murine Embryonic Fibroblasts: E.K. Tribble, et al. ; J. Lipid Res. 57, 1492 (2016), Application(s): Western blot, Abstract; Full Text
Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5: R. Ushioda, et al.; Proc. Natl. Acad. Sci. USA 113, E6055 (2016), Application(s): Immunoprecipitation and immunoblot analysis , Abstract; Full Text
Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons: M. Pigoni, et al.; Mol. Neurodegener. 11, 67 (2016), Application(s): Immunoblotting, mouse brain, Abstract; Full Text
The Oncogenic Fusion Proteins SET-Nup214 and Sequestosome-1 (SQSTM1)-Nup214 Form Dynamic Nuclear Bodies and Differentially Affect Nuclear Protein and Poly(A)+ RNA Export: S.A. Port, et al.; J. Biol. Chem. 291, 23068 (2016), Abstract; Full Text
The Rapamycin-Sensitive Complex of Mammalian Target of Rapamycin Is Essential to Maintain Male Fertility: C. Schell, et al.; Am. J. Pathol. 186, 324 (2016), Abstract;
VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington’s disease: X. Guo, et al.; Nat. Commun. 7, 12646 (2016), Application(s): Cell culture, Abstract;
Cell type- and brain region-resolved mouse brain proteome: P.T. White, et al.; Nat. Neurosci. 18, 1819 (2015), Application(s): Western blot, Abstract;
Characterization and Mechanism of Stress-induced Translocation of 78-kilodalton Glucose Regulated Protein (GRP78) to the Cell Surface: Y.L. Tsai, et al.; J. Biol. Chem. 290, 8049 (2015), Application(s): Western Blotting, Abstract; Full Text
Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer: K.E. Maxfield, et al.; Nat. Commun. 6, 8840 (2015), Application(s): Immunofluorescence, Abstract;
Data supporting ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid β peptide secretion: K. Suga, et al.; Data Brief 5, 782 (2015), Application(s): Western blot, Abstract;
Development-dependent regulation of molecular chaperones after hypoxia-ischemia: X. Sun, et al.; Neurobiol. Dis. 82, 123 (2015), Abstract;
Effect of ionomycin on interaction of calnexin with vesicular stomatitis virus glycoprotein is cell type-specific: M. Okudera, et al. ; J. Oral Sci. 557, 305 (2015), Application(s): Western blot, Abstract; Full Text
Exome sequencing identifies SUCO mutations in mesial temporal lobe epilepsy: Z. Sha, et al.; Neurosci. Lett. 591, 149 (2015), Application(s): Immunocytochemistry, Abstract;
Homer2 within the nucleus accumbens core bidirectionally regulates alcohol intake by both P and Wistar rats: A. Haider, et al.; Alcohol 49, 533 (2015), Application(s): Western Blot, Abstract;
N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum: E. Dudek, et al.; Biochem. Biophys. Res. Commun. 468, 889 (2015), Application(s): Cell culture, Immunofluorescence, Abstract;
UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum: D. Lee, et al.; J. Biol. Chem. 290, 5725 (2015), Abstract; Full Text
Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses: C. Biswas, et al.; J. Biol. Chem. 289, 26882 (2014), Abstract; Full Text
Recycling endosome tubule morphogenesis from sorting endosomes requires the kinesin motor KIF13A: C. Delevoye, et al.; Cell Rep. 6, 445 (2014), Abstract;
Autophagy facilitates organelle clearance during differentiation of human erythroblasts: evidence for a role for ATG4 paralogs during autophagosome maturation: V.M. Betin, et al.; Autophagy 9, 881 (2013), Abstract; Full Text
The nuclear envelope proteome differs notably between tissues: N. Korfali, et al.; Nucleus 3, 552 (2012), Application(s): Western blot, Abstract; Full Text
LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat: M. Knepper, et al. ; Am. J. Physiol. Renal Physiol. 295, F1799 (2008), Application(s): WB using rat cell lysates, Abstract;
Mitofusin 2 tethers endoplasmic reticulum to mitochondria: L. Scorrano, et al. ; Nature 456, 605 (2008), Application(s): IF using human tissue culture, Abstract;
The ectodomain of Toll-like recepto r9 is cleaved to generate a functional receptor: G. Barton, et al. ; Nature 456, 658 (2008), Application(s): WB, EM using mouse cell lysates, Abstract;
Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic β cells: J. Oberwinkler, et al. ; Nat. Cell Biol. 10, 1421 (2008), Application(s): WB using human cell lysates, Abstract;
Specificity of plasma membrane targeting by the rous sarcoma virus gag protein: L.J. Parent, et al. ; J. Virol. 77, 470 (2003), Application(s): ICC using quail samples, Abstract;
Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane: R.S. Hegde, et al. ; J. Cell Biol. 160, 529 (2003), Application(s): WB using canine & porcine samples, Abstract;
Probing for membrane domains in the endoplasmic reticulum: retention and degradation of unassembled MHC class I molecules: M. Edidin, et al. ; Mol. Biol. Cell 13, 1566 (2002), Application(s): IP, ICC using mouse samples, Abstract;
Type XXVI collagen, a new member of the collagen family, is specifically expressed in the testis and ovary: K. Nagata, et al. ; J. Biol. Chem. 277, 37678 (2002), Application(s): ICC using monkey samples, Abstract;
The export of major histocompatibility complex class i molecules from the endoplasmic reticulum of rat brown adipose cells is acutely stimulated by insulin: S.W. Cushman, et al. ; Mol. Biol. Cell 12, 101 (2001), Application(s): ICC using rat samples, Abstract;
T cell receptor assembly and expression in the absence of calnexin: K.P. Kearse, et al. ; Arch. Biochem. Biophys. 378, 182 (2000), Application(s): IP, WB using human samples, Abstract;

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