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Calnexin polyclonal antibody

 
ADI-SPA-860-D 50 µg 201.00 USD
 
ADI-SPA-860-F 200 µg 378.00 USD
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Product Specification

Alternative Name:CNX
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of dog calnexin. The sequence is completely conserved in human, mouse and rat.
 
UniProt ID:P24643
 
GenBank ID:X53616
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Quail, Rabbit, Sheep, Xenopus
 
Applications:ICC, IF, IHC (PS), IP, WB
Electron microscopy
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~90kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Calnexin (CNX), an unglycosylated resident ER transmembrane protein, together with Calreticulin (CRT), plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglucosylated glycans. Calnexin associates with newly synthesized monomeric glycoproteins and only recognizes glycoproteins when they are incompletely folded. Furthermore, Calnexin has been demonstrated to function as a molecular chaperone capable of interacting with polypeptide segments of folding glycoproteins.
 
SPA-860 IHC
Immunohistochemistry analysis of human spleen tissue stained with Calnexin, pAb at 10µg/ml.
ADI-SPA-860 WB
Western blot analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).
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SPA-860 IHC ADI-SPA-860 WB

Product Literature References

KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine: M. Puhka, et al.; Eur. J. Pharm. Sci. 98, 30 (2017), Application(s): Western blot, urine EVs, Abstract;
Grp94 Delivers γ-aminobutyric Acid Type A (GABAA) Receptors to Hrd1-Mediatd Endoplasmic Reticulum-Associated Degradation: X.J. Di, et al.; J. Biol. Chem. 291, 9526 (2016), Application(s): Western blot, Abstract; Full Text
Homer2 regulates alcohol and stress cross-sensitization: S.G. Quadir, et al.; Addict. Biol. 21, 613 (2016), Application(s): Immunoblotting, Abstract;
Identification of VPS13C as a Galectin-12-Binding Protein That Regulates Galectin-12 Protein Stability and Adipogenesis: R.Y. Yang, et al.; PLoS One 11, e0153534 (2016), Application(s): HeLa cells immunostained with calnexin antibody, Abstract; Full Text
Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons: D. Schmitt, et al.; Histochem. Cell Biol. 146, 489 (2016), Application(s): Western blot, rabbit antiserum antibody, Abstract;
Proteolytic processing of Neuregulin 1 type III by three intramembrane cleaving proteases: D. Fleck, et al.; J. Biol. Chem. 291, 318 (2016), Application(s): Loading control, Abstract; Full Text
Quantitative Profiling of the Endonuclear Glycero-Phospholipidome of Murine Embryonic Fibroblasts: E.K. Tribble, et al. ; J. Lipid Res. 57, 1492 (2016), Application(s): Western blot, Abstract; Full Text
Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5: R. Ushioda, et al.; Proc. Natl. Acad. Sci. USA 113, E6055 (2016), Application(s): Immunoprecipitation and immunoblot analysis , Abstract; Full Text
Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons: M. Pigoni, et al.; Mol. Neurodegener. 11, 67 (2016), Application(s): Immunoblotting, mouse brain, Abstract; Full Text
VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington’s disease: X. Guo, et al.; Nat. Commun. 7, 12646 (2016), Application(s): Cell culture, Abstract;
Cell type- and brain region-resolved mouse brain proteome: P.T. White, et al.; Nat. Neurosci. 18, 1819 (2015), Application(s): Western blot, Abstract;
Characterization and Mechanism of Stress-induced Translocation of 78-kilodalton Glucose Regulated Protein (GRP78) to the Cell Surface: Y.L. Tsai, et al.; J. Biol. Chem. 290, 8049 (2015), Application(s): Western Blotting, Abstract; Full Text
Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer: K.E. Maxfield, et al.; Nat. Commun. 6, 8840 (2015), Application(s): Immunofluorescence, Abstract;
Data supporting ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid β peptide secretion: K. Suga, et al.; Data Brief 5, 782 (2015), Application(s): Western blot, Abstract;
Development-dependent regulation of molecular chaperones after hypoxia-ischemia: X. Sun, et al.; Neurobiol. Dis. 82, 123 (2015), Abstract;
Effect of ionomycin on interaction of calnexin with vesicular stomatitis virus glycoprotein is cell type-specific: M. Okudera, et al. ; J. Oral Sci. 557, 305 (2015), Application(s): Western blot, Abstract; Full Text
Exome sequencing identifies SUCO mutations in mesial temporal lobe epilepsy: Z. Sha, et al.; Neurosci. Lett. 591, 149 (2015), Application(s): Immunocytochemistry, Abstract;
Homer2 within the nucleus accumbens core bidirectionally regulates alcohol intake by both P and Wistar rats: A. Haider, et al.; Alcohol 49, 533 (2015), Application(s): Western Blot, Abstract;
N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum: E. Dudek, et al.; Biochem. Biophys. Res. Commun. 468, 889 (2015), Application(s): Cell culture, Immunofluorescence, Abstract;
UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum: D. Lee, et al.; J. Biol. Chem. 290, 5725 (2015), Application(s): Western Blotting, Abstract; Full Text
Recycling endosome tubule morphogenesis from sorting endosomes requires the kinesin motor KIF13A: C. Delevoye, et al.; Cell Rep. 6, 445 (2014), Abstract;
The nuclear envelope proteome differs notably between tissues: N. Korfali, et al.; Nucleus 3, 552 (2012), Application(s): Western blot, Abstract; Full Text
LC-MS/MS analysis of differential centrifugation fractions from native inner medullary collecting duct of rat: M. Knepper, et al. ; Am. J. Physiol. Renal Physiol. 295, F1799 (2008), Application(s): WB using rat cell lysates, Abstract;
Mitofusin 2 tethers endoplasmic reticulum to mitochondria: L. Scorrano, et al. ; Nature 456, 605 (2008), Application(s): IF using human tissue culture, Abstract;
The ectodomain of Toll-like recepto r9 is cleaved to generate a functional receptor: G. Barton, et al. ; Nature 456, 658 (2008), Application(s): WB, EM using mouse cell lysates, Abstract;
Transient receptor potential M3 channels are ionotropic steroid receptors in pancreatic β cells: J. Oberwinkler, et al. ; Nat. Cell Biol. 10, 1421 (2008), Application(s): WB using human cell lysates, Abstract;
Specificity of plasma membrane targeting by the rous sarcoma virus gag protein: L.J. Parent, et al. ; J. Virol. 77, 470 (2003), Application(s): ICC using quail samples, Abstract;
Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane: R.S. Hegde, et al. ; J. Cell Biol. 160, 529 (2003), Application(s): WB using canine & porcine samples, Abstract;
Probing for membrane domains in the endoplasmic reticulum: retention and degradation of unassembled MHC class I molecules: M. Edidin, et al. ; Mol. Biol. Cell 13, 1566 (2002), Application(s): IP, ICC using mouse samples, Abstract;
Type XXVI collagen, a new member of the collagen family, is specifically expressed in the testis and ovary: K. Nagata, et al. ; J. Biol. Chem. 277, 37678 (2002), Application(s): ICC using monkey samples, Abstract;
The export of major histocompatibility complex class i molecules from the endoplasmic reticulum of rat brown adipose cells is acutely stimulated by insulin: S.W. Cushman, et al. ; Mol. Biol. Cell 12, 101 (2001), Application(s): ICC using rat samples, Abstract;
T cell receptor assembly and expression in the absence of calnexin: K.P. Kearse, et al. ; Arch. Biochem. Biophys. 378, 182 (2000), Application(s): IP, WB using human samples, Abstract;

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