HSP90 polyclonal antibody



ADI-SPA-836-D	50 µg	180.00 USD

ADI-SPA-836-F	200 µg	415.00 USD
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Product Details

Alternative Name:	Heat shock protein 90

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to aa 250-325 of human Hsp90.

UniProt ID:	P07900 (HSP90α), P08238 (HSP90β)

Source:	Purified from rabbit serum.

Species reactivity:	Human, Mouse, Rat Rabbit

Applications:	IHC (PS), WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000, Colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~90kDa by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Regulatory Status:	RUO - Research Use Only

HSP90 polyclonal antibody Immunohistochemistry

Immunohistochemistry analysis of human prostate tissue stained with HSP90, pAb at 10µg/ml.

Western blot analysis of HSP90, pAb (Prod. No. ADI-SPA-836): Lane 1: MW marker, Lane 2:HSP90α (human), (rec) (Prod. No. ADI-SPP-776), Lane 3: HSP90β (human), (rec) (Prod. No. ADI-SPP-777), Lane 4: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101), Lane 5: 3T3 (heat shocked) (Prod. No. ADI-LYC-3T101),Lane 6: PC-12 (heat shocked) (Prod. No. ADI-LYC-PC101).

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Product Literature References

Phosphorylation of PBX2, a novel downstream target of mTORC1, is determined by GSK3 and PP1: R. Wada, et al.; J. Biochem. 173, 129 (2023), Abstract;

NAD+-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice: H.W. Kim, et al.; Theranostics 12, 3316 (2022), Abstract;

HTLV-1 viral oncogene HBZ drives bone destruction in adult T cell leukemia: J. Xiang, et al.; JCI Insight 4, e128713 (2019), Abstract; Full Text

The O-GlcNAc Transferase Intellectual Disability Mutation L254F Distorts the TPR Helix: M. Gundogdu, et al.; Cell Chem. Biol. 25, 513 (2018), Abstract;

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