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HSP90 monoclonal antibody (16F1)

 
ADI-SPA-835-D 50 µg 204.00 USD
 
ADI-SPA-835-F 200 µg 409.00 USD
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Product Specification

Alternative Name:Heat shock protein 90
 
Clone:16F1
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Native human Hsp90.
 
UniProt ID:P07900 (HSP90alpha), P08238 (HSP90beta)
 
Source:Purified from ascites.
 
Species reactivity:Human, Mouse, Rat
Beluga, Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Monkey, Mussel, Opossum, Porcine, Plant, Rabbit, Scallop, Sheep, Xenopus
 
Applications:IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~90kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
 
HSP90 monoclonal antibody (16F1) Immunohistochemistry
Immunohistochemistry analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10µg/ml.
HSP90 monoclonal antibody (16F1) Western blot
Western blot analysis of HSP90: Lane 1: MW marker, Lane 2: HSP90 native protein (ADI-SPP-770), Lane 3: HSP90b recombinant protein (Prod. No. ADI-SPP-772), Lane 4: HSP90alpha recombinant protein (Prod. No. ADI-SPP-776), Lane 5: HeLa (HS), Lane 6: L-929 (HS), Lane 7: Rat-2 (HS), Lane 8:RK-13 (HS).
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HSP90 monoclonal antibody (16F1) Immunohistochemistry HSP90 monoclonal antibody (16F1) Western blot

Product Literature References

Hypoxia induced hERG trafficking defect linked to cell cycle arrest in SH-SY5Y cells: D.R. Vaddi, et al.; PLoS One 14, e0215905 (2019), Abstract; Full Text
Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90: R.A. Sager, et al.; Cell Rep. 26, 1344 (2019), Abstract; Full Text
Detection and Analysis of Extracellular Hsp90 (eHsp90): S. Cortes, et al.; Methods Mol. Biol. 1709, 321 (2018), Abstract;
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci. 19, E178 (2018), Application(s):Western Blot, Abstract; Full Text
Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot: D.A. Nikishin, et al.; J. Assist. Reprod. Genet. 35, 1851 (2018), Abstract;
Sporadic renal angiomyolipoma in a patient with Birt-Hogg-Dubé: chaperones in pathogenesis: R.A. Sager, et al.; Oncotarget 9, 22220 (2018), Abstract; Full Text
Histone deacetylase activity mediates acquired resistance towards structurally diverse HSP90 inhibitors: R.C. Chai, et al.; Mol. Oncol. 11, 567 (2017), Abstract; Full Text
HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death: A.C. Jacobsen, et al.; Cell Death Dis. 14, e2051 (2016), Application(s): Western blots of both mouse and human cell lines, Abstract;
HSP90 regulates temperature-dependent seedling growth in Arabidopsis by stabilizing the auxin co-receptor F-box protein TIR1: R. Wang, et al.; Nat. Commun. 7, 10269 (2016), Application(s): Immunoprecipitation, Abstract;
Inverse Relationship between Progesterone Receptor and Myc in Endometrial Cancer: T. Kavlashvili, et al.; PLoS One 11, e0148912 (2016), Abstract; Full Text
Recombinant HSP70 and mild heat shock stimulate growth of aged mesenchymal stem cells: N.V. Andreeva, et al.; Cell Stress Chaperones 21, 727 (2016), Abstract; Full Text
Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines: F. Clemente-Vicario, et al.; PLoS One 10, e0142007 (2015), Application(s): Immunoblot analyses, Abstract; Full Text
Identification and quantitative analysis of cellular proteins affected by treatment with withaferin A using A SILAC-based proteomics approach: M. Narayan, et al.; J. Ethnopharmacol. 8741, 30149 (2015), Application(s): Western Blot, Abstract;
Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain: M. M. Pearce, et al.; Nat. Commun. 6, 6768 (2015), Application(s): Immunofluorescence , Abstract;
The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling: L. Wu, et al.; J. Immunol. 193, 1590 (2014), Abstract;
Psoriasis-associated variant Act1 D10N with impaired regulation by Hsp90: C. Wang, et al.; Nat. Immunol. 14, 72 (2013), Application(s): WB using MEF and HEK293 extracts, Abstract; Full Text
Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase: M.G. Hasham, et al.; J. Immunol. 189, 2374 (2012), Application(s): WB using mouse splenic B-cells extracts, Abstract;
Methylglyoxal alters the function and stability of critical components of the protein quality control: C. F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
Amyloid-β 1-42 induced endocytosis and clusterin/apoJ protein accumulation in cultured human astrocytes: A. Salminen, et al. ; Neurochem. Int. 50, 540 (2007), Application(s): WB using human cell lysates, Abstract;
Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology 64, 1940 (2005), Application(s): Immunohistochemistry on testis tissue, Abstract;

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