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HSP90 monoclonal antibody (16F1)

ADI-SPA-835-D 50 µg 204.00 USD
ADI-SPA-835-F 200 µg 409.00 USD
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Product Specification

Alternative Name:Heat shock protein 90
Immunogen:Native human Hsp90.
UniProt ID:P07900 (HSP90alpha), P08238 (HSP90beta)
Species reactivity:Human, Mouse, Rat
Beluga, Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Monkey, Mussel, Opossum, Porcine, Plant, Rabbit, Scallop, Sheep, Xenopus
Applications:IHC (PS), IP, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~90kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
SPA-835 WB
Western blot analysis of HSP90: Lane 1: MW marker, Lane 2: HSP90 native protein (ADI-SPP-770), Lane 3: HSP90b recombinant protein (Prod. No. ADI-SPP-772), Lane 4: HSP90alpha recombinant protein (Prod. No. ADI-SPP-776), Lane 5: HeLa (HS), Lane 6: L-929 (HS), Lane 7: Rat-2 (HS), Lane 8:RK-13 (HS).
Immunohistochemistry analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10µg/ml.
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SPA-835 WB SPA-835 IHC

Product Literature References

Detection and Analysis of Extracellular Hsp90 (eHsp90): S. Cortes, et al.; Methods Mol. Biol. 1709, 321 (2018), Abstract;
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci. 19, E178 (2018), Application(s):Western Blot, Abstract; Full Text
Sporadic renal angiomyolipoma in a patient with Birt-Hogg-Dubé: chaperones in pathogenesis: R.A. Sager, et al.; Oncotarget 9, 22220 (2018), Abstract; Full Text
HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death: A.C. Jacobsen, et al.; Cell Death Dis. 14, e2051 (2016), Application(s): Western blots of both mouse and human cell lines, Abstract;
HSP90 regulates temperature-dependent seedling growth in Arabidopsis by stabilizing the auxin co-receptor F-box protein TIR1: R. Wang, et al.; Nat. Commun. 7, 10269 (2016), Application(s): Immunoprecipitation, Abstract;
Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell Lines: F. Clemente-Vicario, et al.; PLoS One 10, e0142007 (2015), Application(s): Immunoblot analyses, Abstract; Full Text
Identification and quantitative analysis of cellular proteins affected by treatment with withaferin A using A SILAC-based proteomics approach: M. Narayan, et al.; J. Ethnopharmacol. 8741, 30149 (2015), Application(s): Western Blot, Abstract;
Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain: M. M. Pearce, et al.; Nat. Commun. 6, 6768 (2015), Application(s): Immunofluorescence , Abstract;
The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling: L. Wu, et al.; J. Immunol. 193, 1590 (2014), Abstract;
Psoriasis-associated variant Act1 D10N with impaired regulation by Hsp90: C. Wang, et al.; Nat. Immunol. 14, 72 (2013), Application(s): WB using MEF and HEK293 extracts, Abstract; Full Text
Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase: M.G. Hasham, et al.; J. Immunol. 189, 2374 (2012), Application(s): WB using mouse splenic B-cells extracts, Abstract;
Methylglyoxal alters the function and stability of critical components of the protein quality control: C. F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
Amyloid-β 1-42 induced endocytosis and clusterin/apoJ protein accumulation in cultured human astrocytes: A. Salminen, et al. ; Neurochem. Int. 50, 540 (2007), Application(s): WB using human cell lysates, Abstract;
Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology 64, 1940 (2005), Application(s): Immunohistochemistry on testis tissue, Abstract;

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