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HSP90 monoclonal antibody (2D11B9)

 
ADI-SPA-831-050 50 µg 194.00 USD
 
ADI-SPA-831-200 200 µg 389.00 USD
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Product Specification

Alternative Name:Heat shock protein 90
 
Clone:2D11B9
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Hsp90.
 
UniProt ID:P07900 (HSP90α), P08238 (HSP90β)
 
Species reactivity:Human, Mouse, Rat
Dog, Drosophila, Fish, Guinea pig, Hamster, Horse, Monkey, Porcine, Sheep
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~92kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The 90kDa molecular chaperone family includes 90kDa heat shock protein Hsp90 and 94kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90α are 85% and 90% homologous to those of Hsp90β, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signalling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophilia Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits, even after Hsp90 function is restored.
 
ADI-SPA-831 WB
Western Blot Analysis of HSP90, mAb (2D11B9) (Prod. No. ADI-SPA-831). Lane 1: MWM; Lane 2: HSP90 (human), (native) (Prod. No. ADI-SPP-770); Lane 3: HeLa, (cell lysate) (Prod. No. ADI-LYC-HL100); and Lane 4: HeLa (heat shocked), (cell lysate) (Prod. No. ADI-LYC-HL101).
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ADI-SPA-831 WB

Product Literature References

Limonene reduces hyperalgesia induced by gp120 and cytokines by modulation of IL-1 β and protein expression in spinal cord of mice: A.C. Picinelli, et al.; Life Sci. 174, 28 (2017), Abstract;
Novel HSP90 inhibitors effectively target functions of thyroid cancer stem cell preventing migration and invasion: P.T. White, et al.; Surgery 159, 142 (2015), Application(s): Western blot, Abstract;
Salternamide A Suppresses Hypoxia-Induced Accumulation of HIF-1α and Induces Apoptosis in Human Colorectal Cancer Cells: D.H. Bach, et al.; Mar. Drugs 13, 6976 (2015), Application(s): Immunoblotting of human colorectal cancer cells, Abstract; Full Text

General Literature References

The charged region of Hsp90 modulates the function of the N-terminal domain: T. Scheibel, et al.; PNAS USA 96, 1297 (1999), Abstract;
Hsp90 as a capacitor for morphological evolution: S.L. Rutherford & S. Lindquist; Nature 396, 336 (1998), Abstract;
Oligomeric forms of the 90-kDa heat shock protein: T. Nemoto & N. Sato; Biochem. J. 330, 989 (1998), Abstract;
The Hsp90 complex--a super-chaperone machine as a novel drug target: T. Scheibel & J. Buchner; Biochem. Pharmacol. 56, 675 (1998), Abstract;
Guidebook to Chaperones: T. Scheibel & J. Buchner; Ed. Gething, M.J. Oxford Univ. Press 147 (1997), Book,

Related Products

HeLa (heat shocked) , (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL101-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

HeLa, (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL100-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

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