Product Details
| Alternative Name: | Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine |
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| Clone: | 10C3 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78. |
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| UniProt ID: | P06761 |
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| GenBank ID: | M14050 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Avian, Insect, Mammalian, Plant, Yeast
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| Specificity: | Recognizes proteins containing the KDEL sequence. |
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| Applications: | ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked) (Prod No. ADI-LYC-HL101).
Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).
Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.
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Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers: R.A. Buchwalter, et al.; Cells
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Spray-Dried Formulation of Epicertin, a Recombinant Cholera Toxin B Subunit Variant That Induces Mucosal Healing: M.A. Reeves, et al.; Pharmaceutics
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The novel missense mutation Met48Lys in FKBP22 changes its structure and functions: Y. Ishikawa, et al.; Sci. Rep.
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Proinsulin misfolding is an early event in the progression to type 2 diabetes: A. Arunagiri, et al.; Elife
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Proper secretion of the serpin antithrombin relies strictly on thiol-dependent quality control: B.M. Adams, et al.; J. Biol. Chem.
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Schwann cells ER-associated degradation contributes to myelin maintenance in adult nerves and limits demyelination in CMT1B mice: V.G. Volpi, et al.; PLoS Genet.
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The lysosomal transporter MFSD1 is essential for liver homeostasis and critically depends on its accessory subunit GLMP: D. Massa Lopez, et al.; Elife
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The Multifunctional Sorting Protein PACS-2 Controls Mitophagosome Formation in Human Vascular Smooth Muscle Cells through Mitochondria-ER Contact Sites: M. Moulis, et al.; Cells
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Characterization of Non-amyloidogenic G101S Transthyretin: Y. Wakita, et al.; Biol. Pharm. Bull.
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Exploring the Interaction Between eIF2α Dysregulation, Acute Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian Brain: G. Beauvais, et al.; Neuroscience
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The BAX/BAK-like protein BOK is a prognostic marker in colorectal cancer: S. Carberry, et al.; Cell Death Dis.
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UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism: L. Trobiani, et al.; Neurobiol. Dis.
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BRAF-mutated cells activate GCN2-mediated integrated stress response as a cytoprotective mechanism in response to vemurafenib: I. Nagasawa, et al.; Biochem. Biophys. Res. Commun.
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Disruption of the mitochondria-associated ER membrane (MAM) plays a central role in palmitic acid-induced insulin resistance: S. Shinjo, et al.; Exp. Cell Res.
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eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet: W.G. Choi, et al.; Nutr.
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Human neutrophil elastase induces endothelial cell apoptosis by activating the PERK-CHOP branch of the unfolded protein response: I. Grechowa, et al.; FASEB J.
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Ligand and Target Discovery by Fragment-Based Screening in Human Cells: C.G. Parker, et al.; Cell
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Pofut1 point-mutations that disrupt O-fucosyltransferase activity destabilize the protein and abolish Notch1 signaling during mouse somitogenesis: R. Ajima, et al.; PLoS One
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Sublethal endoplasmic reticulum stress caused by the mutation of immunoglobulin heavy chain-binding protein induces the synthesis of a mitochondrial protein, pyrroline-5-carboxylate reductase 1: H. Jin, et al.; Cell Stress Chaperones
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Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces stress in J774A.1 murine macrophages: A. Mohammadi, et al.; Adv. Med. Sci.
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Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury: M. Onate, et al.; Sci. Rep.
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High expression of α-synuclein in damaged mitochondria with PLA2G6 dysfunction: H. Sumi-Akamaru, et al.; Acta. Neuropathol. Commun.
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Morphology and Molecular Composition of Purified Bovine Viral Diarrhea Virus Envelope: N. Callens, et al.; PLoS One
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mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy: M. Taneike, et al.; PLoS One
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Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa: G. Arno, et al.; Am. J. Hum. Genet.
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T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation: R.P. Laura, et al.; PLoS One.
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A53T Human α-Synuclein Overexpression in Transgenic Mice Induces Pervasive Mitochondria Macroautophagy Defects Preceding Dopamine Neuron Degeneration: L. Chen, et al.; J. Neurosci.
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Cellular localization and tissue distribution of endogenous DFCP1 protein: T. Nanao, et al.; Biomed. Res.
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CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction: D.H. Nam, et al.; J. Mol. Cell. Cardiol.
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Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila: S. Dunst, et al.; Dev. Cell.
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ER stress induces SIRT1 expression via the PI3K-Akt-GSK3β signaling pathway and promotes hepatocellular injury: T. Koga, et al.; J. Biol. Chem.
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FAD-linked Presenilin-1 V97L mutation impede tranport regulation and intracellular Ca2+ homeostasis under ER stress: Y. Shao, et al.; Int. J. Clin. Exp. Med.
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Gefitinib and Erlotinib Lead to Phosphorylation of Eukaryotic Initiation Factor 2 Alpha Independent of Epidermal Growth Factor Receptor in A549 Cells: S. Koyama, et al.; PLoS One
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GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses: Y. Hotokezaka, et al.; Cell Death Dis.
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Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE: K. Saito, et al.; Eur. J. Immunol.
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Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia: A.V. Pinho, et al.; PLoS One
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PINK1 and Parkin Control Localized Translation of Respiratory Chain Component mRNAs on Mitochondria Outer Membrane: S. Gehrke, et al.; Cell Metab.
21, 95 (2015),
Application(s): Western Blotting,
Abstract;
The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin: M. Brunati, et al.; Elife
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Abstract;
Full Text
Activation of the Proapoptotic Unfolded Protein Response in Plaques of the Human Carotid Artery: B. Dorweiler, et al.; Eur. J. Vasc. Endovasc. Surg.
48, 248 (2014),
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Abstract;
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase: T. Takayanagi, et al.; Clin. Sci. (Lond.)
126, 785 (2014),
Abstract;
Full Text
Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic β-cells: E. Boslem, et al.; J. Biol. Chem.
288, 26569 (2013),
Application(s): Immunocytochemistry and Western blot using murine pancreatic islet and MIN6 cells,
Abstract;
Full Text
Down-Regulation of Survivin by Nemadipine-A Sensitizes Cancer Cells to TRAIL-Induced Apoptosis: S. Park, et al.; Biomol. Ther.
21, 29 (2013),
Abstract;
Full Text
The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes: K.R. Lind, et al.; Neurochem. Int.
62, 784 (2013),
Abstract;
Full Text
Analysis of the endoplasmic reticulum subproteome in the livers of type 2 diabetic mice: E.C. Park, et al.; Int. J. Mol. Sci.
13, 17230 (2012),
Abstract;
Full Text
Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion: C.C. Glembotski, et al.; J. Biol. Chem.
287, 25893 (2012),
Abstract;
Full Text
Endoplasmic Reticulum Stress Induces Leptin Resistance: K. Ozawa, et al. ; Mol. Pharmacol.
74, 1610 (2008),
Application(s): WB using mouse & human cell lysates,
Abstract;
Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins: S. Tan, et al. ; J. Biol. Chem.
283, 32621 (2008),
Application(s): IF using human tissue culture,
Abstract;
Pofut1 is required for the proper localization of the Notch receptor during mouse development: Y. Saga, et al. ; Mech. Dev.
125, 663 (2008),
Application(s): IF using mouse tissue,
Abstract;
The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway: D. Sitterlin, et al. ; J. Biol. Chem.
283, 32352 (2008),
Application(s): IP using human cell lysates,
Abstract;
Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases: D. Ito, et al. ; Ann. Neurol.
61, 237 (2007),
Application(s): WB, ICC, IF, IHC using human & mouse cell lysates & tissue,
Abstract;
Tissue and subcellular distribution of CLIC1: B. Ulmasov, et al.; BMC Cell Biol.
8, 8 (2007),
Abstract;
Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila: J. Culi & R.S. Mann; Cell
112, 343 (2003),
Abstract;
Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity: R.C. Austin, et al. ; J. Biol. Chem.
278, 17438 (2003),
Application(s): WB using human samples,
Abstract;
The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis: H.M. Prehn, et al. ; J. Neurochem.
87, 248 (2003),
Application(s): WB using rat samples,
Abstract;
A protein sequence that can encode native structure by disfavoring alternate conformations: W.C. Wigley, et al.; Nat. Struct. Biol.
9, 381 (2002),
Abstract;
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae: K.J. Kauffman, et al.; Biotechnol. Prog.
18, 942 (2002),
Application(s): WB using yeast samples,
Abstract;
Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion: S.E. Dryer, et al. ; J. Neurophysiol.
88, 954 (2002),
Application(s): ICC using chicken samples,
Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: S. Kathuria, et al.; Hum. Reprod.
17, 2054 (2002),
Application(s): WB, EIA using plant (tobacco) samples,
Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: N. Shastri, et al. ; Nature
419, 480 (2002),
Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: T. Serwold, et al.; Nature
419, 480 (2002),
Application(s): WB, ICC using monkey samples,
Abstract;
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds: K. Toyooka, et al.; J. Cell Biol.
148, 453 (2000),
Abstract;
Identification of amino acids in the binding pocket of the human KDEL receptor: A.A. Scheel & H.R. Pelham; J. Biol. Chem.
273, 2467 (1998),
Abstract;
Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins: A. Choukhi, et al.; J. Virol.
72, 3851 (1998),
Abstract;
Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis: V.I. Titorenko & R.A. Rachubinski; Mol. Cell Biol.
18, 2789 (1998),
Abstract;
Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal: D. Vaux, et al.; Nature
345, 495 (1990),
Abstract;
The retention signal for soluble proteins of the endoplasmic reticulum: H.R. Pelham; Trends Biochem. Sci.
15, 483 (1990),
Abstract;
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