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KDEL monoclonal antibody (10C3)

ADI-SPA-827-D 50 µg 204.00 USD
ADI-SPA-827-F 200 µg 431.00 USD
ADI-SPA-827-J 1 mg 1,645.00 USD
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Product Specification

Alternative Name:Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
Immunogen:Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
UniProt ID:P06761
GenBank ID:M14050
Source:Purified from ascites.
Species reactivity:Avian, Insect, Mammalian, Plant, Yeast
Specificity:Recognizes proteins containing the KDEL sequence.
Applications:ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot..
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.
SPA-827 IF
Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).
SPA-827 WB 01
Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked) (Prod No. ADI-LYC-HL101).
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SPA-827 IHC SPA-827 IF SPA-827 WB 01

Product Literature References

Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2α pathway dysregulation in DYT1 transgenic rats in vivo: G. Beauvais, et al.; Brain Res. 1706, 24 (2019), Abstract;
IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages: E.A. Ayaub, et al.; Immunol. Cell Biol. 97, 203 (2019), Abstract;
Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis: E. A. Blackwood, et al.; Nat. Commun. 10, 187 (2019), Abstract; Full Text
Characterization of Non-amyloidogenic G101S Transthyretin: Y. Wakita, et al.; Biol. Pharm. Bull. 41, 628 (2018), Abstract;
Exploring the Interaction Between eIF2α Dysregulation, Acute Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian Brain: G. Beauvais, et al.; Neuroscience 371, 455 (2018), Abstract;
Laminar Flow Inhibits ER Stress-Induced Endothelial Apoptosis through PI3K/Akt-Dependent Signaling Pathway: S. Kim, et al.; Mol. Cells 41, 964 (2018), Abstract; Full Text
Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response: P. Gomez-Fernandez, et al.; Front. Immunol. 9, 2934 (2018), Abstract; Full Text
Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained: P. Lebeau, et al.; J. Biol. Chem. 293, 7329 (2018), Abstract;
Nitazoxanide inhibits paramyxovirus replication by targeting the Fusion protein folding: role of glycoprotein-specific thiol oxidoreductase ERp57: S. Piacentini, et al.; Sci. Rep. 8, 10425 (2018), Abstract; Full Text
Rab44, a novel large Rab GTPase, negatively regulates osteoclast differentiation by modulating intracellular calcium levels followed by NFATc1 activation: Y. Yamaguchi, et al.; Cell. Mol. Life Sci. 75, 33 (2018), Abstract;
The BAX/BAK-like protein BOK is a prognostic marker in colorectal cancer: S. Carberry, et al.; Cell Death Dis. 9, 125 (2018), Application(s):Western Blot, Abstract; Full Text
UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism: L. Trobiani, et al.; Neurobiol. Dis. 120, 139 (2018), Abstract;
BRAF-mutated cells activate GCN2-mediated integrated stress response as a cytoprotective mechanism in response to vemurafenib: I. Nagasawa, et al.; Biochem. Biophys. Res. Commun. 482, 1491 (2017), Abstract;
Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A: J. Zhang, et al.; J. Biol. Chem. 292, 10328 (2017), Abstract; Full Text
Disruption of the mitochondria-associated ER membrane (MAM) plays a central role in palmitic acid-induced insulin resistance: S. Shinjo, et al.; Exp. Cell Res. 359, 86 (2017), Abstract;
eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet: W.G. Choi, et al.; Nutr. 14, 48 (2017), Abstract; Full Text
ER Stress Induced by Tunicamycin Triggers α-Synuclein Oligomerization, Dopaminergic Neurons Death and Locomotor Impairment: a New Model of Parkinson's Disease: V. Coppola-Segovia, et al.; Mol. Neurobiol. 54, 5798 (2017), Abstract;
Human neutrophil elastase induces endothelial cell apoptosis by activating the PERK-CHOP branch of the unfolded protein response: I. Grechowa, et al.; FASEB J. 31, 3868 (2017), Abstract;
Ligand and Target Discovery by Fragment-Based Screening in Human Cells: C.G. Parker, et al.; Cell 168, 527 (2017), Abstract; Full Text
Mutant uromodulin expression leads to altered homeostasis of the endoplasmic reticulum and activates the unfolded protein response: C. Schaeffer, et al.; PLoS One 12, e0175970 (2017), Abstract; Full Text
Pofut1 point-mutations that disrupt O-fucosyltransferase activity destabilize the protein and abolish Notch1 signaling during mouse somitogenesis: R. Ajima, et al.; PLoS One 12, e0187248 (2017), Application(s): IHC, Abstract; Full Text
Sublethal endoplasmic reticulum stress caused by the mutation of immunoglobulin heavy chain-binding protein induces the synthesis of a mitochondrial protein, pyrroline-5-carboxylate reductase 1: H. Jin, et al.; Cell Stress Chaperones 22, 77 (2017), Abstract; Full Text
Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces stress in J774A.1 murine macrophages: A. Mohammadi, et al.; Adv. Med. Sci. 63, 57 (2017), Application(s): Western Blot, Abstract;
A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargos: H.C. Tie, et al.; Mol. Biol. Cell 27, 848 (2016), Application(s): Tagging on the Golgi, Abstract; Full Text
Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury: M. Onate, et al.; Sci. Rep. 6, 21709 (2016), Application(s): Histological analysis, Abstract; Full Text
Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system: L. Ulbrich, et al.; J. Biochem. 473, 423 (2016), Abstract; Full Text
Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion: M. Ueda, et al.; Biochem. Biophys. Res. Commun. 474, 259 (2016), Abstract;
High expression of α-synuclein in damaged mitochondria with PLA2G6 dysfunction: H. Sumi-Akamaru, et al.; Acta. Neuropathol. Commun. 4, 27 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells: Y. Niwa, et al.; Mol. Biol. Cell 27, 744 (2016), Application(s): Immunofluorescence, Abstract; Full Text
Morphology and Molecular Composition of Purified Bovine Viral Diarrhea Virus Envelope: N. Callens, et al.; PLoS One 12, e1005476 (2016), Application(s): Western blot, Abstract; Full Text
mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy: M. Taneike, et al.; PLoS One 11, e0152628 (2016), Abstract; Full Text
Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa: G. Arno, et al.; Am. J. Hum. Genet. 99, 1305 (2016), Abstract; Full Text
T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation: R.P. Laura, et al.; PLoS One. 11, e0149391 (2016), Application(s): Western blot, Abstract; Full Text
A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue: J. Merulla, et al.; Mol. Biol. Cell 26, 1532 (2015), Abstract; Full Text
A53T Human α-Synuclein Overexpression in Transgenic Mice Induces Pervasive Mitochondria Macroautophagy Defects Preceding Dopamine Neuron Degeneration: L. Chen, et al.; J. Neurosci. 35, 890 (2015), Application(s): Immunofluorescence, Abstract;
Cellular localization and tissue distribution of endogenous DFCP1 protein: T. Nanao, et al.; Biomed. Res. 36, 121 (2015), Application(s): Immunocytochemistry, Abstract; Full Text
CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction: D.H. Nam, et al.; J. Mol. Cell. Cardiol. 85, 168 (2015), Abstract;
Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila: S. Dunst, et al.; Dev. Cell. 33, 351 (2015), Application(s): Immunohistochemistry, Abstract;
ER stress induces SIRT1 expression via the PI3K-Akt-GSK3β signaling pathway and promotes hepatocellular injury: T. Koga, et al.; J. Biol. Chem. 290, 10143 (2015), Application(s): Western Blot, Abstract;
FAD-linked Presenilin-1 V97L mutation impede tranport regulation and intracellular Ca2+ homeostasis under ER stress: Y. Shao, et al.; Int. J. Clin. Exp. Med. 8, 20742 (2015), Application(s): Immunoblot analysis, Abstract; Full Text
Gefitinib and Erlotinib Lead to Phosphorylation of Eukaryotic Initiation Factor 2 Alpha Independent of Epidermal Growth Factor Receptor in A549 Cells: S. Koyama, et al.; PLoS One 10, e0136176 (2015), Abstract; Full Text
GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses: Y. Hotokezaka, et al.; Cell Death Dis. 6, e1719 (2015), Application(s): Western Blot, Abstract; Full Text
Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia: A.V. Pinho, et al.; PLoS One 10, e0128012 (2015), Application(s): Immunofluorescence, Abstract; Full Text
PINK1 and Parkin Control Localized Translation of Respiratory Chain Component mRNAs on Mitochondria Outer Membrane: S. Gehrke, et al.; Cell Metab. 21, 95 (2015), Application(s): Western Blotting, Abstract;
The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin: M. Brunati, et al.; Elife 4, e08887 (2015), Abstract; Full Text
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol. 97, 518 (2015), Application(s): Western Blot, Abstract;
Activation of the Proapoptotic Unfolded Protein Response in Plaques of the Human Carotid Artery: B. Dorweiler, et al.; Eur. J. Vasc. Endovasc. Surg. 48, 248 (2014), Application(s): Immunohistochemistry using formalin-fixed paraffin-embedded human carotid artery tissue samples, Abstract;
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase: T. Takayanagi, et al.; Clin. Sci. (Lond.) 126, 785 (2014), Abstract; Full Text
Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic β-cells: E. Boslem, et al.; J. Biol. Chem. 288, 26569 (2013), Application(s): Immunocytochemistry and Western blot using murine pancreatic islet and MIN6 cells, Abstract; Full Text
Down-Regulation of Survivin by Nemadipine-A Sensitizes Cancer Cells to TRAIL-Induced Apoptosis: S. Park, et al.; Biomol. Ther. 21, 29 (2013), Abstract; Full Text
The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes: K.R. Lind, et al.; Neurochem. Int. 62, 784 (2013), Abstract; Full Text
Analysis of the endoplasmic reticulum subproteome in the livers of type 2 diabetic mice: E.C. Park, et al.; Int. J. Mol. Sci. 13, 17230 (2012), Abstract; Full Text
Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion: C.C. Glembotski, et al.; J. Biol. Chem. 287, 25893 (2012), Abstract; Full Text
Endoplasmic Reticulum Stress Induces Leptin Resistance: K. Ozawa, et al. ; Mol. Pharmacol. 74, 1610 (2008), Application(s): WB using mouse & human cell lysates, Abstract;
Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins: S. Tan, et al. ; J. Biol. Chem. 283, 32621 (2008), Application(s): IF using human tissue culture, Abstract;
Pofut1 is required for the proper localization of the Notch receptor during mouse development: Y. Saga, et al. ; Mech. Dev. 125, 663 (2008), Application(s): IF using mouse tissue, Abstract;
The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway: D. Sitterlin, et al. ; J. Biol. Chem. 283, 32352 (2008), Application(s): IP using human cell lysates, Abstract;
Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases: D. Ito, et al. ; Ann. Neurol. 61, 237 (2007), Application(s): WB, ICC, IF, IHC using human & mouse cell lysates & tissue, Abstract;
Tissue and subcellular distribution of CLIC1: B. Ulmasov, et al.; BMC Cell Biol. 8, 8 (2007), Abstract;
Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila: J. Culi & R.S. Mann; Cell 112, 343 (2003), Abstract;
Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity: R.C. Austin, et al. ; J. Biol. Chem. 278, 17438 (2003), Application(s): WB using human samples, Abstract;
The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis: H.M. Prehn, et al. ; J. Neurochem. 87, 248 (2003), Application(s): WB using rat samples, Abstract;
A protein sequence that can encode native structure by disfavoring alternate conformations: W.C. Wigley, et al.; Nat. Struct. Biol. 9, 381 (2002), Abstract;
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae: A.S. Robinson, et al. ; Biotechnol. Prog. 18, 942 (2002), Application(s): WB using yeast samples, Abstract;
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Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion: S.E. Dryer, et al. ; J. Neurophysiol. 88, 954 (2002), Application(s): ICC using chicken samples, Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: R. Finnern, et al. ; Hum. Reprod. 17, 2054 (2002), Application(s): WB, EIA using plant (tobacco) samples, Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: S. Kathuria, et al.; Hum. Reprod. 17, 2054 (2002), Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: N. Shastri, et al. ; Nature 419, 480 (2002), Application(s): WB, ICC using monkey samples, Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: T. Serwold, et al.; Nature 419, 480 (2002), Abstract;
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds: K. Toyooka, et al.; J. Cell Biol. 148, 453 (2000), Abstract;
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Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins: A. Choukhi, et al.; J. Virol. 72, 3851 (1998), Abstract;
Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis: V.I. Titorenko & R.A. Rachubinski; Mol. Cell Biol. 18, 2789 (1998), Abstract;
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