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KDEL monoclonal antibody (10C3)

ADI-SPA-827-D 50 µg 198.00 USD
ADI-SPA-827-F 200 µg 418.00 USD
ADI-SPA-827-J 1 mg 1,597.00 USD
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Product Specification

Alternative Name:Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
Immunogen:Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
UniProt ID:P06761
GenBank ID:M14050
Source:Purified from ascites.
Species reactivity:Avian, Insect, Mammalian, Plant, Yeast
Specificity:Recognizes proteins containing the KDEL sequence.
Applications:ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot..
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.
SPA-827 IF
Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).
SPA-827 WB 01
Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked) (Prod No. ADI-LYC-HL101).
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SPA-827 IHC SPA-827 IF SPA-827 WB 01

Product Literature References

Nitazoxanide inhibits paramyxovirus replication by targeting the Fusion protein folding: role of glycoprotein-specific thiol oxidoreductase ERp57: S. Piacentini, et al.; Sci. Rep. 8, 10425 (2018), Abstract; Full Text

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