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KDEL monoclonal antibody (10C3)

ADI-SPA-827-D 50 µg 194.00 USD
ADI-SPA-827-F 200 µg 410.00 USD
ADI-SPA-827-J 1 mg 1,566.00 USD
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Product Specification

Alternative Name:Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
Immunogen:Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
UniProt ID:P06761
GenBank ID:M14050
Species reactivity:Avian, Insect, Mammalian, Plant, Yeast
Specificity:Recognizes proteins containing the KDEL sequence.
Applications:ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot..
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.
SPA-827 IF
Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).
SPA-827 WB 01
Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked) (Prod No. ADI-LYC-HL101).
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SPA-827 IHC SPA-827 IF SPA-827 WB 01

Product Literature References

BRAF-mutated cells activate GCN2-mediated integrated stress response as a cytoprotective mechanism in response to vemurafenib: I. Nagasawa, et al.; Biochem. Biophys. Res. Commun. 482, 1491 (2017), Abstract;
A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargos: H.C. Tie, et al.; Mol. Biol. Cell 27, 848 (2016), Application(s): Tagging on the Golgi, Abstract; Full Text
Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury: M. Onate, et al.; Sci. Rep. 6, 21709 (2016), Application(s): Histological analysis, Abstract; Full Text
Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion: M. Ueda, et al.; Biochem. Biophys. Res. Commun. 474, 259 (2016), Abstract;
High expression of α-synuclein in damaged mitochondria with PLA2G6 dysfunction: H. Sumi-Akamaru, et al.; Acta. Neuropathol. Commun. 4, 27 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells: Y. Niwa, et al.; Mol. Biol. Cell 27, 744 (2016), Application(s): Immunofluorescence, Abstract; Full Text
Morphology and Molecular Composition of Purified Bovine Viral Diarrhea Virus Envelope: N. Callens, et al.; PLoS One 12, e1005476 (2016), Application(s): Western blot, Abstract; Full Text
T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation: R.P. Laura, et al.; PLoS One. 11, e0149391 (2016), Application(s): Western blot, Abstract; Full Text
A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue: J. Merulla, et al.; Mol. Biol. Cell 26, 1532 (2015), Abstract; Full Text
A53T Human α-Synuclein Overexpression in Transgenic Mice Induces Pervasive Mitochondria Macroautophagy Defects Preceding Dopamine Neuron Degeneration: L. Chen, et al.; J. Neurosci. 35, 890 (2015), Application(s): Immunofluorescence, Abstract;
Cellular localization and tissue distribution of endogenous DFCP1 protein: T. Nanao, et al.; Biomed. Res. 36, 121 (2015), Application(s): Immunocytochemistry, Abstract; Full Text
CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction: D.H. Nam, et al.; J. Mol. Cell. Cardiol. 85, 168 (2015), Abstract;
Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila: S. Dunst, et al.; Dev. Cell. 33, 351 (2015), Application(s): Immunohistochemistry, Abstract;
ER stress induces SIRT1 expression via the PI3K-Akt-GSK3β signaling pathway and promotes hepatocellular injury: T. Koga, et al.; J. Biol. Chem. 290, 10143 (2015), Application(s): Western Blot, Abstract;
FAD-linked Presenilin-1 V97L mutation impede tranport regulation and intracellular Ca2+ homeostasis under ER stress: Y. Shao, et al.; Int. J. Clin. Exp. Med. 8, 20742 (2015), Application(s): Immunoblot analysis, Abstract; Full Text
GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses: Y. Hotokezaka, et al.; Cell Death Dis. 6, e1719 (2015), Application(s): Western Blot, Abstract; Full Text
Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia: A.V. Pinho, et al.; PLoS One 10, e0128012 (2015), Application(s): Immunofluorescence, Abstract; Full Text
PINK1 and Parkin Control Localized Translation of Respiratory Chain Component mRNAs on Mitochondria Outer Membrane: S. Gehrke, et al.; Cell Metab. 21, 95 (2015), Application(s): Western Blotting, Abstract;
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol. 97, 518 (2015), Application(s): Western Blot, Abstract;
Activation of the Proapoptotic Unfolded Protein Response in Plaques of the Human Carotid Artery: B. Dorweiler, et al.; Eur. J. Vasc. Endovasc. Surg. 48, 248 (2014), Application(s): Immunohistochemistry using formalin-fixed paraffin-embedded human carotid artery tissue samples, Abstract;
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase: T. Takayanagi, et al.; Clin. Sci. (Lond.) 126, 785 (2014), Abstract;
Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic β-cells: E. Boslem, et al.; J. Biol. Chem. 288, 26569 (2013), Application(s): Immunocytochemistry and Western blot using murine pancreatic islet and MIN6 cells, Abstract; Full Text
Endoplasmic Reticulum Stress Induces Leptin Resistance: K. Ozawa, et al. ; Mol. Pharmacol. 74, 1610 (2008), Application(s): WB using mouse & human cell lysates, Abstract;
Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins: S. Tan, et al. ; J. Biol. Chem. 283, 32621 (2008), Application(s): IF using human tissue culture, Abstract;
Pofut1 is required for the proper localization of the Notch receptor during mouse development: Y. Saga, et al. ; Mech. Dev. 125, 663 (2008), Application(s): IF using mouse tissue, Abstract;
The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway: D. Sitterlin, et al. ; J. Biol. Chem. 283, 32352 (2008), Application(s): IP using human cell lysates, Abstract;
Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases: D. Ito, et al. ; Ann. Neurol. 61, 237 (2007), Application(s): WB, ICC, IF, IHC using human & mouse cell lysates & tissue, Abstract;
Tissue and subcellular distribution of CLIC1: B. Ulmasov, et al.; BMC Cell Biol. 8, 8 (2007), Abstract;
Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila: J. Culi & R.S. Mann; Cell 112, 343 (2003), Abstract;
Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity: R.C. Austin, et al. ; J. Biol. Chem. 278, 17438 (2003), Application(s): WB using human samples, Abstract;
The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis: H.M. Prehn, et al. ; J. Neurochem. 87, 248 (2003), Application(s): WB using rat samples, Abstract;
A protein sequence that can encode native structure by disfavoring alternate conformations: W.C. Wigley, et al.; Nat. Struct. Biol. 9, 381 (2002), Abstract;
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae: A.S. Robinson, et al. ; Biotechnol. Prog. 18, 942 (2002), Application(s): WB using yeast samples, Abstract;
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae: K.J. Kauffman, et al.; Biotechnol. Prog. 18, 942 (2002), Abstract;
Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion: S.E. Dryer, et al. ; J. Neurophysiol. 88, 954 (2002), Application(s): ICC using chicken samples, Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: R. Finnern, et al. ; Hum. Reprod. 17, 2054 (2002), Application(s): WB, EIA using plant (tobacco) samples, Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: S. Kathuria, et al.; Hum. Reprod. 17, 2054 (2002), Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: N. Shastri, et al. ; Nature 419, 480 (2002), Application(s): WB, ICC using monkey samples, Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: T. Serwold, et al.; Nature 419, 480 (2002), Abstract;
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds: K. Toyooka, et al.; J. Cell Biol. 148, 453 (2000), Abstract;
Identification of amino acids in the binding pocket of the human KDEL receptor: A.A. Scheel & H.R. Pelham; J. Biol. Chem. 273, 2467 (1998), Abstract;
Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins: A. Choukhi, et al.; J. Virol. 72, 3851 (1998), Abstract;
Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis: V.I. Titorenko & R.A. Rachubinski; Mol. Cell Biol. 18, 2789 (1998), Abstract;
Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal: D. Vaux, et al.; Nature 345, 495 (1990), Abstract;
The retention signal for soluble proteins of the endoplasmic reticulum: H.R. Pelham; Trends Biochem. Sci. 15, 483 (1990), Abstract;

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