KDEL, mAb (10C3) (DyLight^TM 488 conjugate)

Alternative Name:	Grp78, Grp94, Lysine-aspartic acid-glutamate-leucine

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS containing 0.09% sodium azide.

Clone:	10C3

Isotype:	Mouse IgG2a

Immunogen:	Synthetic peptide corresponding to aa 649-654 (S⁶⁴⁹EKDEL⁶⁵⁴) of rat Grp78.

Specificity:	Recognizes proteins containing the KDEL sequence in mammals, birds, insects and and plants. Cross reacts with yeast proteins containing the KDEL sequence. Detects bands of ~55kDa, 78kDa and ~94kDa by Western blot.

Application:	Flow Cytometry (1:20) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.