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Grp78/BiP polyclonal antibody

ADI-SPA-826-D 50 µg 164.00 USD
ADI-SPA-826-F 200 µg 363.00 USD
ADI-SPA-826-J 1 mg 1,300.00 USD
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Product Specification

Alternative Name:HspA5, HSP70-5, Glucose-regulated protein 78, Heat shock 70kDa protein 5
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of rat Grp78 (BiP). The sequence is completely conserved in mouse and hamster.
UniProt ID:P06761
GenBank ID:M14050
Species reactivity:Mouse, Rat
Bovine, Fungus, Hamster, Monkey, Rabbit, Xenopus
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~78kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Grp78 (Glucose Regulated 78kDa Protein), is also known as BiP or Immunoglobulin heavy chain binding protein. It is a stressresponse protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding, and assembly of many membrane bound and secreted proteins as well as for maintenance of cell homeostasis and the prevention of apoptosis.
SPA-826 WB
Western blot analysis: Lane 1: MW marker, Lane 2: 150 ng of Grp78 (BiP) recombinant protein (Prod. No. ADI-SPP-765), Lane 3: 20µg of CHO-K1 cell lysate, Lane 4: 20µg of mouse liver microsome tissue extract (Prod. No. ADI-LYT-MM100).
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SPA-826 WB

Product Literature References

Involvement of Up-Regulation of DR5 Expression and Down-Regulation of c-FLIP in Niclosamide-Mediated TRAIL Sensitization in Human Renal Carcinoma Caki Cells: J.M. Yun, et al.; Molecules 23, E2264 (2018), Abstract; Full Text
Protective effect of 4-Phenylbutyrate against proteolipid protein mutation-induced endoplasmicreticulum stress and oligodendroglial cell death: A.S. Wilding, et al.; Neurochem. Int. 118, 185 (2018), Abstract;
SIRT1 activation with neuroheal is neuroprotective but SIRT2 inhibition with AK7 is detrimental for disconnected motoneurons: D. Romeo-Guitartet, al.; Cell Death Dis. 9, 531 (2018), Abstract; Full Text
Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury: M. Onate, et al.; Sci. Rep. 6, 21709 (2016), Application(s): Western blot, Abstract; Full Text
The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development: A.L. Joly, et al.; Oncogene 35, 2842 (2016), Application(s): Western Blot, Abstract;
A Versatile Cell Death Screening Assay Using Dye-Stained Cells and Multivariate Image Analysis: T.J. Collins, et al. ; Assay Drug Dev. Technol. 13, 547 (2015), Application(s): Western Blot, Abstract; Full Text
MiR-184 regulates pancreatic β-cell function according to glucose metabolism: S.G. Tattikota, et al.; J. Biol. Chem. 290, 20284 (2015), Application(s): Western Blot, Abstract; Full Text
ERp29, an Endoplasmic Reticulum Secretion Factor Is Involved in the Growth of Breast Tumor Xenografts: S. Mkrtchian, et al. ; Mol. Carcinog. 47, 886 (2008), Application(s): WB using human tissue culture, Abstract;
Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med. 12, 2482 (2008), Application(s): WB using rat cell lysates, Abstract;
Small heat-shock proteins select DeltaF508-CFTR for endoplasmic reticulum-associated degradation: J. Brodsky, et al. ; Mol. Biol. Cell 18, 806 (2007), Application(s): WB using human cell lysates, Abstract;
Bax plays a pivotal role in thapsigargin-induced apoptosis of human colon cancer HCT116 cells by controlling Smac/Diablo and Omi/HtrA2 release from mitochondria: H.G. Wang, et al. ; Cancer Res. 63, 1483 (2003), Application(s): WB using human samples, Abstract;
gamma-Secretase activity requires the presenilin-dependent trafficking of nicastrin through the Golgi apparatus but not its complex glycosylation: B. De Strooper, et al. ; J. Cell. Sci. 116, 1127 (2003), Application(s): ICC using mouse samples, Abstract;
Mutant SOD1 linked to familial amyotrophic lateral sclerosis, but not wild-type SOD1, induces ER stress in COS7 cells and transgenic mice: T. Kato, et al. ; Biochem. Biophys. Res. Commun. 303, 496 (2003), Application(s): ICC, WB, IHC using monkey samples, Abstract;
Differential interaction of molecular chaperones with procollagen I and type IV collagen in corneal endothelial cells: E.P. Kay, et al. ; Mol. Vis. 8, 9 (2002), Application(s): ICC using rabbit samples, Abstract;
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP: M. Mori, et al. ; J. Biol. Chem. 277, 12343 (2002), Application(s): WB using mouse samples, Abstract;
The protein kinase/endoribonuclease IRE1 alpha that signals the unfolded protein response has a luminal N-terminal ligand-independent dimerization domain: R.J. Kaufman, et al. ; J. Biol. Chem. 277, 18346 (2002), Application(s): IP using human & monkey samples, Abstract;
Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells: T.S. McClintock, et al. ; J. Neurochem. 72, 2301 (1999), Application(s): ICC using human & xenopus samples, Abstract;

General Literature References

The critical roles of endoplasmic reticulum chaperones and unfolded protein response in tumorigenesis and anticancer therapies: B. Luo & A.S. Lee; Oncogene 32, 805 (2013), (Review), Abstract; Full Text
The critical role of GRP78 in physiologic and pathologic stress: K.T. Pfaffenbach & A.S. Lee; Curr. Opin. Cell Biol. 23, 150 (2011), (Review), Abstract; Full Text

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