Replaces Prod. #: BML-SA661
Product Details
| Alternative Name: | Hsc70/Hsp73, Hsp70/Hsp72 |
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| Clone: | N27F3-4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Purified HSC70 (Hsp73) and HSP70 (Hsp72) isolated from human HeLa cells. |
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| UniProt ID: | P0DMV8 (HSP70/HSP72), P11142 (HSC70/HSP73) |
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| GenBank ID: | M11717 (HSP70), Y00371 (HSC70) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Beluga, Bovine, Chicken, Dog, Fish, Guinea pig, Hamster, Monkey, Porcine, Plant, Rabbit, Sheep, Xenopus
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| Applications: | Flow Cytometry, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:100)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~72-73kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HSC70/HSP70, mAb (N27F3-4) (Prod. No. ADI-SPA-820):
Lane 1: MW marker,
Lane 2: HSC70/HSP73 (bovine), (recombinant) (Prod. No. ADI-SPP-751),
Lane 3: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555),
Lane 4: 3T3,
Lane 5: 3T3 (heat shocked),
Lane 6: HeLa,
Lane 7: HeLa (heat shocked),
Lane 8: PC-12,
Lane 9: PC-12 (heat shocked).
Flow cytometry analysis of human colon cancer Caco2 cells analyzed by flow cytometry using isotype control.
Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP70, mAb (N27F3-4) at 10µg/ml.
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Product Literature References
Identification of Suitable Internal Control miRNAs in Bovine Milk Small Extracellular Vesicles for Normalization in Quantitative Real-Time Polymerase Chain Reaction: M.M. Rahman, et al.; Membranes
13, 185 (2023),
Abstract;
Identification of Potential mRNA Biomarkers in Milk Small Extracellular Vesicles of Enzootic Bovine Leukosis Cattle: M. Hiraoka, et al.; Viruses
14, 1022 (2022),
Abstract;
EndoBind detects endogenous protein-protein interactions in real time: A. Bill, et al.; Commun. Biol.
15, 1085 (2021),
Abstract;
Plasma extracellular vesicles in people living with HIV and type 2 diabetes are related to microbial translocation and cardiovascular risk: B. Vestad, et al.; Sci. Rep.
11, 21936 (2021),
Abstract;
A Legionella pneumophila Kinase Phosphorylates the Hsp70 Chaperone Family to Inhibit Eukaryotic Protein Synthesis: S.M. Moss, et al.; Cell Host Microbe
25, 454 (2019),
Abstract;
Biallelic mutation in MYH7 and MYBPC3 leads to severe cardiomyopathy with left ventricular noncompaction phenotype: K. Kolokotronis, et al.; Hum. Mutat.
40, 1101 (2019),
Abstract;
Endothelial edema precedes blood-brain barrier breakdown in early time points after experimental focal cerebral ischemia: M. Krueger, et al.; Acta Neuropathol. Commun.
7, 17 (2019),
Application(s): Fluoresence microscopy on mouse neuronal nuclei in tissue ,
Abstract;
Full Text
Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens: S.H. Wang, et al.; Anim. Biotechnol.
19, 1 (2018),
Abstract;
Stress in native grasses under ecologically relevant heat waves: M. Davies, et al.; PLoS One
13, e0204906 (2018),
Abstract;
Full Text
Evidence that Hsc70 is associated with CNV particles and plays a role in CNV particle disassembly: S.B. Alam & D. Rochon; J. Virol.
91, e01555 (2017),
Abstract;
Targeted delivery of chemotherapy using HSP90 inhibitor drug conjugates is highly active against pancreatic cancer models: E. Bobrov, et al.; Oncotarget
8, 4399 (2017),
Abstract;
Full Text
A Novel HSP90 Inhibitor–Drug Conjugate to SN38 Is Highly Effective in Small Cell Lung Cancer: A.V. Gaponova, et al.; Clin. Cancer Res.
22, 5120 (2016),
Abstract;
Full Text
MicroRNA deregulation in triple negative breast cancer reveals a role of miR-498 in regulating BRCA1 expression: N. Matamala, et al.; Oncotarget
7, 20068 (2016),
Application(s): Western blot,
Abstract;
Using heat shock proteins to improve the therapeutic benefit of a non-vaccine treatment modality: P. Srivastava, et al.; (2016), (U.S. patent),
Application(s): Immunoblotting,
Full Text
Anti-interleukin-6 therapy through application of a monogenic protein inhibitor via gene delivery: D. Gortz, et al.; Sci. Rep.
5, 14685 (2015),
Abstract;
Full Text
Autophagy supports survival and phototransduction protein levels in rod photoreceptors: Z. Zhou, et al.; Cell Death Differ.
22, 488 (2015),
Application(s): Western Blotting,
Abstract;
Exosomes, endogenous retroviruses and toll-like receptors: pregnancy recognition in ewes: I. Ruiz-Gonzalez, et al.; Reproduction.
149, 281 (2015),
Application(s): Immunohistochemistry on uterine tissue,
Abstract;
Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress: T. Mulaudzi-Masuku, et al.; Cell Stress Chaperones
20, 793 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Localization and expression of heat shock protein 70 with rat myocardial cell damage induced by heat stress in vitro and in vivo: H. Chen, et al.; Mol. Med. Rep.
11, 2276 (2015),
Abstract;
The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets: A. Di Giancamillo, et al.; J. Anim. Sci.
93, 2849 (2015),
Application(s): Immunohistochemistry on liver tissue,
Abstract;
Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice: Z.B. Smithline, et al.; PLoS One
9, e114403 (2014),
Application(s): Fluorescent Western blot,
Abstract;
Full Text
Hsp70 and Hsp90 expression in citrus and pepper plants in response to Xanthomonas axonopodis pv. Citri: J. Ottado, et al. ; Eur. J. Plant Pathol.
123, 91 (2009),
Application(s): WB using plant cell lysates,
Abstract;
High levels of heat shock protein Hsp72 in cancer cells suppress default senescence pathways: M. Sherman, et al. ; Cancer Res.
67, 2373 (2007),
Application(s): WB using human cell lysates,
Abstract;
Loss of beta4 integrin subunit reduces the tumorigenicity of MCF7 mammary cells and causes apoptosis upon hormone deprivation: G. Bon, et al.; Clin. Cancer. Res.
12, 3280 (2006),
Application(s): Immunocytochemistry,
Abstract;
Full Text
Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology
64, 1940 (2005),
Application(s): Immunohistochemistry on testis tissue,
Abstract;
Co-translational folding of caspase-activated DNase with Hsp70, Hsp40, and inhibitor of caspase-activated DNase: S. Nagata, et al. ; J. Biol. Chem.
277, 3364 (2002),
Application(s): WB using rabbit samples,
Abstract;
Heat shock protein 70 moderately enhances peptide binding and transport by the transporter associated with antigen processing: M.J. Androlewicz, et al. ; Immunol. Lett.
75, 143 (2001),
Application(s): IP using human samples,
Abstract;
Heat shock protein 70 is able to prevent heat shock-induced resistance of target cells to CTL: E. Gunther, et al. ; J. Immunol.
164, 2362 (2000),
Application(s): WB using rat samples,
Abstract;
Monoclonal antibodies raised against infectious haematopoietic necrosis virus (IHNV) G protein and a cellular 90 kDa protein neutralize IHNV infection in vitro: J.Y. Lee, et al.; J. Gen. Virol.
77, 1731 (1996),
Application(s): Western blot analysis compared to 90 kDa protein,
Abstract;
Full Text
Hormone and antihormone induce distinct conformational changes which are central to steroid receptor activation: G.F. Allan, et al.; J. Biol. Chem.
267, 19513 (1992),
Application(s): Flow using human samples,
Abstract;
Binding of heat shock proteins to the avian progesterone receptor: S.L. Kost, et al.; Mol. Cell. Biol.
9, 3829 (1989),
Application(s): WB using chicken samples,
Abstract;
Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus: J.B. Winfield, et al. ; J. Exp. Med.
168, 1475 (1988),
Application(s): WB using human samples,
Abstract;
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