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HSC70/HSP73 monoclonal antibody (1B5)

 
ADI-SPA-815-D 50 µg 194.00 USD
 
ADI-SPA-815-F 200 µg 389.00 USD
 
ADI-SPA-815-J 1 mg 1,566.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Heat shock protein 70, Hsc70, Hsp73
 
Clone:1B5
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Native hamster Hsc70 (Hsp73).
 
UniProt ID:P19378
 
GenBank ID:M34561
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Turtle
 
Applications:ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~73kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
 
SPA-815 IHC
Immunohistochemistry analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10µg/ml.
SPA-815 WB
Western blot analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) (Prod No. ADI-SPP-751) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) (Prod No. ADI-LYC-3T100) Lane 5: PC-12, (Cell Lysate), (Prod No. ADI-LYC-PC100), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate) (Prod No. ADI-LYC-HL100).
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SPA-815 IHC SPA-815 WB

Product Literature References

ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation: J.H. Seo, et al.; Nat. Commun. 7, 12882 (2016), Application(s): In vitro acetylation assay, deacetylation assay, binding assay, and ATPase activity assay, Abstract; Full Text
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development: K. Uchiyama, et al.; Sci. Rep. 6, 32094 (2016), Application(s): Western blot, mouse colonic epithelial YAMC cells, Abstract; Full Text
Engineering hybrid exosomes by membrane fusion with liposomes: Y.T. Sato, et al.; Sci. Rep. 6, 21933 (2016), Application(s): Western blot, Abstract; Full Text
Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis: M.K. Stewart, et al.; PLoS One 11, e0154162 (2016), Application(s): Western blot, Abstract; Full Text
β-Secretase 1's Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice: D. Piedrahita, et al.; Front Cell Neurosci. 9, 498 (2016), Application(s): Western blot, Abstract; Full Text
Dependence of Intracellular and Exosomal microRNAs on Viral E6/E7 Oncogene Expression in HPV-positive Tumor Cells: A. Honegger, et al.; PLoS Pathog. 11, e1004712 (2015), Application(s): Western Blotting, Abstract; Full Text
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy: J. Vasco Ferreira, et al.; Sci. Rep. 5, 10210 (2015), Application(s): Immunoprecipitation, Abstract; Full Text
Keratin 8 absence downregulates colonocyte HMGCS2 and modulates colonic ketogenesis and energy metabolism: T.O. Helenius, et al.; Mol. Biol. Cell 26, 2298 (2015), Application(s): Western Blot, Abstract; Full Text
NDR Kinases Are Essential for Somitogenesis and Cardiac Looping during Mouse Embryonic Development: D. Schmitz-Rohmer, et al.; PLoS One 10, e0136566 (2015), Application(s): Western Blot, Abstract; Full Text
Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat.: A.M. Gleixner, et al.; Cell Tissue Res. 357, 43 (2014), Application(s): Western blot using rat substantia nigra and striatium tissue samples, Abstract;
STUB1 mutations in autosomal recessive ataxias–evidence for mutation-specific clinical heterogeneity: K. Heimdal, et al.; Orphanet. J. Rare Dis. 9, 146  (2014), Application(s): Western blot using in vitro ubiquitination assays, Abstract; Full Text
Silencing of human papillomavirus (HPV) E6/E7 oncogene expression affects both the contents and the amounts of extracellular microvesicles released from HPV-positive cancer cells: A. Honegger, et al.; Int. J. Cancer 133, 1631 (2013), Application(s): Western blot using human HeLa cervical carcinoma cells, Abstract; Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Methylglyoxal alters the function and stability of critical components of the protein quality control: C. F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens: H. Kasamatsu, et al. ; J. Virol. 83, 37 (2008), Application(s): WB, IP, IHC using monkey cell lysates, Abstract;
Exogenous androgen treatment delays the stress response following hamster facial nerve injury: K. Jones, et al. ; J. Neuroendocrinol. 19, 383 (2007), Application(s): IHC using hamster tissue, Abstract;
Fever-like hyperthermia controls T lymphocyte persistence by inducing degradation of cellular FLIPshort: J. Eriksson, et al. ; J. Immunol. 178, 3944 (2007), Application(s): WB using human cell lysates, Abstract;
Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking: D. Brown, et al. ; J. Biol. Chem. 282, 28721 (2007), Application(s): WB, IP, IF, EM, ICC using rat cell lysates & tissue, Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): WB, IF using rat tissue, Abstract;
Heat-shock protein90 complexes in resting and thrombin-activated platelets: R.L. Gear, et al. ; Biochem. Biophys. Res. Commun. 297, 129 (2002), Application(s): IP using human samples, Abstract;
Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy: D.E. Merry, et al. ; Hum. Mol. Genet. 11, 515 (2002), Application(s): ICC using mouse samples, Abstract;
Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing: H. Udono, et al. ; J. Exp. Med. 196, 185 (2002), Application(s): WB using mouse samples, Abstract;
The synthetic peptide P-197 inhibits human T-cell leukemia virus type 1 envelope-mediated syncytium formation by a mechanism that is independent of Hsc70: S.R. Jassal, et al. ; J. Virol. 75, 10472 (2001), Application(s): Flow using human samples, Abstract;
HSC70 interactions with SV40 viral proteins differ between permissive and nonpermissive mammalian cells: I. Lazaridis, et al. ; Cell Stress Chaperones 5, 132 (2000), Application(s): ICC using mouse samples, Abstract;

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