Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

HSP70/HSP72 (human) monoclonal antibody (N21F3-2)

 
ADI-SPA-8134-D 50 µg 170.00 USD
 
ADI-SPA-8134-F 200 µg 360.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72
 
Clone:N21F3-2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Native human Hsp70.
 
UniProt ID:P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)
 
GenBank ID:M11717
 
Gene/Protein Identifier:NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM)
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~70kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The 70 kDa heat shock protein Hsp70 belongs to the Hsp70 family of highly-related protein isoforms ranging in size from 66 kDa to 78 kDa. Hsc70 shares close biochemical and biological ties to Hsp70, and also belongs to the Hsp70 family. These proteins include cognate members found within major intracellular compartments and highly inducible isoforms predominantly cytoplasmic or nuclear in distribution. Members of the Hsp70 family function as molecular chaperones involved in such cellular functions as protein folding, transport, maturation and degradation, operating in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains or partially folded intermediates of proteins, preventing their aggregation and misfolding, and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. Data demonstrates that with a ubiquitin-like domain at its amino terminus and its association with the 26S proteosome in HeLa cells, Bag-1 modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal Bag-1's role as a physical link between the Hsc70/Hsp70 chaperone system and the proteasome. Experimental data also shows that the ATPase domain and the substrate-binding domain of Hsp70 (or Hsc70) cooperate to form a co-chaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp), essential for normal neurotransmitter release.
 
SPA-8134 WB
Western blot analysis of HSP70 mAb: Lane 1: HSP70 Recombinant Human Protein, Lane 2: HSP70B’ Recombinant Human Protein, Lane3: HSP70-A1 Recombinant Mouse Protein, Lane 4: HSP70 Recombinant Chinook Salmon Protein, Lane 5: HSC70 Recombinant Bovine Protein, Lane 6: Hsc70 (ATPase Fragment) Recombinant Bovine Protein, Lane 7: HeLa Cell Lysate, Lane 8: HeLa Cell Lysate, Heat Shocked.
Please mouse over
SPA-8134 WB

Related Products

Goat anti-mouse IgG F(ab')2, polyclonal antibody (HRP conjugate) 

ELISA, IHC, WB | Print as PDF
 
ADI-SAB-100-J 1 ml 118.00 USD
Do you need bulk/larger quantities?
 

HeLa, (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL100-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

HeLa (heat shocked) , (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL101-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
HSP70
By biological activity:
HSP70 Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,