Immunoprecipitation (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~70kDa by Western blot.
Purity Detail:
Protein A affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Technical Info/Product Notes:
Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000204389).
Regulatory Status:
RUO - Research Use Only
Western blot analysis of HSP70/HSP72 pAb (Prod. No. ADI-SPA-812): Lane 1: MW marker; Lane 2: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555); Lane 3: PC-12; Lane 4: PC-12 (heat shocked); Lane 5: HeLa; Lane 6: HeLa (heat shocked).
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Product Literature References
Partially hydrolyzed guar gum upregulates heat shock protein 27 in intestinal Caco-2 cells and mouse intestine via mTOR and ERK signaling: D.M. Rini, et al.; J. Sci. Food Agric. 103, 5165 (2023), Abstract;
PolyQ-Expanded Mutant Huntingtin Forms Inclusion Body Following Transient Cold Shock in a Two-Step Aggregation Mechanism: A.R. Castro e Costa, et al.; ACS Chem. Neurosci. 14, 277 (2023), Abstract;
A novel C-terminal heat shock protein 90 inhibitor that overcomes STAT3-Wnt-β-catenin signaling-mediated drug resistance and adverse effects: H.J. Lee, et al.; Theranostics 12, 105 (2022), Abstract;
Fibroblasts as an experimental model system for the study of comparative physiology: C.B. Madelaire, et al.; Comp. Biochem. Physiol. B Biochem. Mol. Bio. 260, 110735 (2022), Abstract;
MicroRNA-124-3p-enriched small extracellular vesicles as a therapeutic approach for Parkinson’s disease: M. Esteves, et al.; Mol. Ther. 30, 3176 (2022), Abstract;
The Change in Circadian Rhythms in P301S Transgenic Mice is Linked to Variability in Hsp70-related Tau Disaggregation: S.M. Han, et al.; Exp. Neurobiol. 31, 196 (2022), Abstract;
The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells: M. Oroń, et al.; Cell Rep. 40, 111428 (2022), Abstract;
Curcumin increases heat shock protein 70 expression via different signaling pathways in intestinal epithelial cells: G. Mingzu, et al.; Arch. Biochem. Biophys. 707, 108938 (2021), Abstract;
FOXR1 regulates stress response pathways and is necessary for proper brain development: A. Mota, et al.; PLoS Genet. 17, e1009854 (2021), Abstract;
Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice: A.R. Caldwell, et al.; J. Appl. Physiol. 10, 1152 (2021), Abstract;
Three-dimensional, multifunctional neural interfaces
for cortical spheroids and engineered assembloids: Y. Park, et al.; Sci. Adv. 7, 9153 (2021), Abstract;
Buxus alkaloid compound destabilizes mutant p53 through inhibition of the HSF1 chaperone axis: Y.L. Wang, et al.; Phytomedicine 68, 153187 (2020), Abstract;
Changes in Heat Stress Tolerance in a Freshwater Amphipod Following Starvation: The Role of Oxygen Availability, Metabolic Rate, Heat Shock Proteins and Energy Reserves: M. Semsar-Kazerouni, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 245, 110697 (2020), Abstract;
Heat Shock and HSP70 Regulate 5-FU-mediated caspase-1 Activation in Myeloid-Derived Suppressor Cells and Tumor Growth in Mice: T. Pilot, et al.; J. Immunother. Cancer 8, e000478 (2020), Abstract; Full Text
Response to oxidative stress of peripheral blood mononuclear cells from multiple sclerosis patients and healthy controls: C. Pistono, et al.; Cell Stress Chaperones 25, 81 (2020), Abstract;
Ticagrelor Enhances Release of Anti-Hypoxic Cardiac Progenitor Cell-Derived Exosomes Through Increasing Cell Proliferation In Vitro: V. Casieri, et al.; Sci. Rep. 10, 2494 (2020), Abstract; Full Text
Hsp70 and Hsp40 inhibit an inter-domain interaction necessary for transcriptional activity in the androgen receptor: B. Eftekharzadeh, et al.; Nat. Commun. 10, 3562 (2019), Abstract; Full Text
HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Dis. 10, 256 (2019), Abstract; Full Text
HSP72 expression is specific to skeletal muscle contraction type: J.P. Bonello & M. Locke; Cell Stress Chaperones 24, 709 (2019), Abstract; Full Text
LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors: Y.L. Fu, et al.; Biochem. Biophys. Res. Commun. 511, 356 (2019), Abstract;
The impact of age and frailty on skeletal muscle autophagy markers and specific strength: A cross-sectional comparison: S.N. Aas, et al.; Exp. Gerontol. 125, 110687 (2019), Abstract;
Functional genomics study of acute heat stress response in the small yellow follicles of layer-type chickens: C.Y. Cheng, et al.; Sci. Rep. 8, 1320 (2018), Abstract; Full Text
Modulation of Alzheimer's amyloid β peptide oligomerization and toxicity by extracellular Hsp70: I. Rivera, et al.; Cell Stress Chaperones 23, 269 (2018), Abstract; Full Text
Histone deacetylase activity mediates acquired resistance towards structurally diverse HSP90 inhibitors: R.C. Chai, et al.; Mol. Oncol. 11, 567 (2017), Abstract; Full Text
Quantification of heat shock protein 70 and acetylcholinesterase over a time course suggests environmental adaptation in a foundational molluscan species: A. Ravaschiere, et al.; Ecotoxicol. Environ. Saf. 142, 222 (2017), Abstract;
Astaxanthin intake attenuates muscle atrophy caused by immobilization in rats: T. Shibaguchi, et al.; Physiol. Rep. 4, e12885 (2016), Application(s): SDS-PAGE and western blotting, Abstract; Full Text
BIIB021, a synthetic Hsp90 inhibitor, induces mutant ataxin-1 degradation through the activation of heat shock factor 1: Y. Ding, et al.; Neuroscience 327, 20 (2016), Application(s): Western blot, Abstract;
Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells: M. Kodiha, et al.; Nanobiomedicine (Rij.) 3, 3 (2016), Application(s): Western blot, Abstract; Full Text
HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth: K.H. Su, et al.; Nat. Cell Biol. 18, 527 (2016), Abstract; Full Text
The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development: A.L. Joly, et al.; Oncogene 35, 2842 (2016), Application(s): Western Blot, Abstract;
Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes: R.P. Hobbs, et al.; Nat. Genet. 47, 933 (2015), Application(s): Western Blot, Abstract;
Malfunction of spermatogenesis in experimental ischemic mice: F. Yazama, et al.; J. Repro. Dev. 61, 399 (2015), Application(s): Immunocytochemistry, Abstract; Full Text
Modulation of inflammatory response in mice with severe autoimmune disease by thymic peptide thymulin and an inhibitor of NF-kappaB signalling: S.M. Lunin, et al.; Int. Immunopharmacol. 25, 260 (2015), Application(s): Western Blotting, Abstract;
Protein:protein Interactions Modulate the Docking-Dependent E3-Ubiquitin Ligase Activity of CHIP: V. Narayan, et al.; Mol. Cell. Proteomics 14, 2973 (2015), Application(s): Binding Assays, Abstract; Full Text
Signaling, stress response and apoptosis in pre-diabetes and diabetes: restoring immune balance in mice with alloxan-induced type 1 diabetes mellitus: E.G. Novoselova, et al.; Int. Immunopharmacol. 31, 24 (2015), Application(s): Western blot, Abstract;
AICAR-induced activation of AMPK negatively regulates myotube hypertrophy through the HSP72-mediated pathway in C2C12 skeletal muscle cells: T. Egawa, et al.; Am. J. Physiol. Endocrinol. Metab. 306, E344 (2014), Application(s): WB using mouse cell lysate, Abstract; Full Text
Directed Expression of a Chimeric Type II Keratin Partially Rescues Keratin 5-null Mice: D.M. Alvarado & P.A. Coulombe; J. Biol. Chem. 289, 19435 (2014), Abstract;
Hsp25 and Hsp72 content in rat skeletal muscle following controlled shortening and lengthening contractions: A.M. Holwerda, et al.; Appl. Physiol. Nutr. Metab. 39, 1380 (2014), Abstract;
Molecular Stress-inducing Compounds Increase Osteoclast Formation in a Heat Shock Factor 1 Protein-dependent Manner: R.C. Chai, et al.; J. Biol. Chem. 289, 13602 (2014), Abstract;
Attenuated thermoregulatory, metabolic, and liver acute phase protein response to heat stroke in TNF receptor knockout mice: L.R. Leon, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 305, R1421 (2013), Abstract;
Biochemical Characterization of Protein Quality Control Mechanisms during Disease Progression in the C22 Mouse Model of CMT1A: V.G. Chittoor, et al.; ASN Neuro 5, AN20130024 (2013), Application(s): WB using mouse sciatic nerve lysates, Abstract; Full Text
Hsp70 and a Novel Axis of Type I Interferon-Dependent Antiviral Immunity in the Measles Virus-Infected Brain: M.Y. Kim, et al.; J. Virol. 87, 998 (2013), Application(s): Hsp70 neutralization in cell culture supernatants from infected mouse microglial BV-2 cells, Abstract; Full Text
Regeneration of injured skeletal muscle in heat shock transcription factor 1-null mice: S. Nishizawa, et al.; PHY2 1, e00071 (2013), Application(s): WB using mouse muscle lysates, Abstract; Full Text
Hsp70 Protein Positively Regulates Rabies Virus Infection: X. Lahaye, et al.; J. Virol. 86, 4743 (2012), Application(s): WB and IP using rabies virus infected hamster BSR cells, Abstract; Full Text
Huntingtin fragments and SOD1 mutants form soluble oligomers in the cell: Y.N. Park, et al.; PLoS One 7, e40329 (2012), Application(s): WB using N2A and HeLA cells, Abstract; Full Text
Myocardial accumulation and localization of the inducible 70-kDa heat shock protein, Hsp70, following exercise: K.J. Milne, et al.; J. Appl. Physiol. 113, 853 (2012), Application(s): IHC using rat heart left ventricle samples, Abstract; Full Text
Absence of heat shock transcription factor 1 retards the regrowth of atrophied soleus muscle in mice: K. Yasuhara, et al.; J. Appl. Physiol. 111, 1142 (2011), Application(s): WB using mouse muscle lysates, Abstract; Full Text
Neuroprotective Drug Riluzole Amplifies the Heat Shock Factor 1 (HSF1)- and Glutamate Transporter 1 (GLT1)-dependent Cytoprotective Mechanisms for Neuronal Survival: A.Y.C. Liu, et al.; J. Biol. Chem. 286, 2785 (2011), Application(s): WB and ICC using mouse/rat NG108-15 and N2a cells, Abstract; Full Text
Replication of the Rotavirus Genome Requires an Active Ubiquitin-Proteasome System: T. López, et al.; J. Virol. 85, 11964 (2011), Application(s): WB using rotavirus RRV infected green monkey epithelial cell line MA104, Abstract; Full Text
Role of HSP101 in the stimulation of nodal root development from the coleoptilar node by light and temperature in maize (Zea mays L.) seedlings: G. López-Frías, et al.; J. Exp. Bot. 62, 4661 (2011), Application(s): WB using maize lysates, Abstract; Full Text
Fibroblast phenotype in male carriers of FMR1 premutation alleles: D. Garcia-Arocena, et al.; Hum. Mol. Genet. 19, 299 (2010), Application(s): WB using human skin fibroblast lysates, Abstract; Full Text
Genetic determinants of HSP70 gene expression following heat shock: N. Maugeri, et al.; Hum. Mol. Genet. 19, 4939 (2010), Application(s): ELISA using human EBV-transformed lymphoblastoid cell line (LCL) lysates, Abstract; Full Text
Intracellular or extracellular heat shock protein 70 differentially regulates cardiac remodelling in pressure overload mice: W.-F. Cai, et al.; Cardiovasc. Res. 88, 140 (2010), Application(s): ELISA using mouse serum, Abstract; Full Text
Long-term survival following a single treatment of kidney tumors with multiwalled carbon nanotubes and near-infrared radiation: A. Burke, et al.; PNAS 106, 12897 (2009), Application(s): IHC using mouse RENCA cell tumor samples, Abstract; Full Text
Tau deletion exacerbates the phenotype of Niemann–Pick type C mice and implicates autophagy in pathogenesis: C.D. Pacheco, et al.; Hum. Mol. Genet. 18, 956 (2009), Application(s): WB using mouse liver lysates, Abstract; Full Text
The Endoplasmic Reticulum-associated Degradation of Transthyretin Variants Is Negatively Regulated by BiP in Mammalian Cells: S. Susuki, et al.; J. Biol. Chem. 284, 8312 (2009), Application(s): WB using human HeLa cell lysates, Abstract; Full Text
Chaperone hsp27, a novel subunit of AUF1 protein complexes, functionsin AU-rich element-mediated mRNA decay: G. Brewer, et al. ; Mol. Cell. Biol. 28, 5223 (2008), Application(s): WB using human cell lysates, Abstract;
CHIP Targets Toxic α-Synuclein Oligomers for Degradation: J.E. Tetzlaff, et al.; J. Biol. Chem. 283, 17962 (2008), Application(s): WB using human brain H4 cell line lysates, Abstract; Full Text
Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli.: A.N. Kavazis, et al.; Am. J. Physiol. Heart Circ. Physiol. 294, H928 (2008), Application(s): WB using rat mitochondria and heart tissue lysates, Abstract; Full Text
Impact of seasonal variation on HSP70 expression quantitated instressed fish hepatocytes: E. Padmini, et al. ; Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 151, 278 (2008), Application(s): IHC using fish cell lysates, Abstract;
Insulin-induced myocardial protection in isolated ischemic rat hearts requires p38 MAPK phosphorylation of Hsp27: G. Li, et al.; Am. J. Physiol. Heart Circ. Physiol. 294, H74 (2008), Application(s): IHC using rat cardiac tissue samples, Abstract; Full Text
Localization and Importance of the Adenovirus E4orf4 Protein during Lytic Infection: K.S. Sinsimer, et al.; Mol. Cell. Biol. 28, 5223 (2008), Application(s): WB using human promonocytic leukemia cell line THP-1 lysates, Abstract; Full Text
Thymulin, A Thymic Peptide, Prevents the Overproduction of Pro-Inflammatory Cytokines and Heat Shock Protein Hsp70 inInflammation-Bearing Mice: E. Novoselova, et al. ; Immunol. Invest. 37, 858 (2008), Application(s): WB using mouse cell lysates, Abstract;
Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking: D. Brown, et al. ; J. Biol. Chem. 282, 28721 (2007), Application(s): IP, WB, ICC, IHC using rat cell lysates & tissue, Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): IF using rat tissue, Abstract;
Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis: R.E. Lee, et al. ; Apoptosis 12, 1183 (2007), Application(s): WB using drosophila cell lysates, Abstract;
Detection of novel intracellular alpha-synuclein oligomeric species by fluorescence lifetime imaging: P. McLean, et al. ; FASEB J. 20, 2050 (2006), Application(s): WB using human cell lysates, Abstract;
A molecular chaperone that assists in the folding of emerging polypeptides and the refolding of denatured proteins.
Produced in E. coli., ≥95% (SDS-PAGE; Western blot), ELISA, IHC, WB, Activity assay, Antigen microarray, Apoptosis assay, in vitro Assay | Print as PDF
