Product Details
| Alternative Name: | Chaperonin 60, CPN60, HspD1, heat shock protein 60 |
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| Clone: | LK-2 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human HSP60. |
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| UniProt ID: | P10809 |
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| GenBank ID: | M34664 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, C. elegans, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Plant, Rabbit, Ehrlichia sennetsu
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| Applications: | ELISA, Flow Cytometry, IHC, IP, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~60kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing: P. Clemente, et al.; Nat. Commun.
13, 5750 (2022),
Abstract;
Physiological responses (Hsps 60 and 32, caspase 3, H2O2 scavenging, and photosynthetic activity) of the coral Pocillopora damicornis under thermal and high nitrate stresses: M. Thummasan, et al.; Mar. Pollut. Bull.
171, 112737 (2021),
Abstract;
Modulation of Hsp60 in response to coral brown band disease: D. Seveso, et al.; Mar. Environ. Res.
115, 15 (2017),
Abstract;
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones
22, 225 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Hsp60 expression profiles in the reef-building coral Seriatopora caliendrum subjected to heat and cold shock regimes: D. Seveso, et al.; Mar. Environ. Res.
119, 1 (2016),
Application(s): Western Blot,
Abstract;
TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes: J.P. Heiserman, et al.; Cell Stress Chaperones
20, 527 (2015),
Abstract;
Full Text
The susceptibility of corals to thermal stress by analyzing Hsp60 expression: D. Seveso, et al.; Mar. Environ. Res.
99, 69 (2014),
Application(s): Western Blot,
Abstract;
HuR and the bioenergetic signature of breast cancer: a low tumor expression of the RNA-binding protein predicts a higher risk of disease recurrence: J. Cuezva, et al. ; Carcinogenesis
29, 2053 (2008),
Application(s): IP using human cell lysates,
Abstract;
Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology
64, 1940 (2005),
Application(s): Western blot using porcine testis,
Abstract;
Differential sublocalization of the dynamin-related protein OPA1 isoforms in mitochondria: H. Endo, et al. ; Biochem. Biophys. Res. Commun.
300, 482 (2003),
Application(s): WB using human samples,
Abstract;
Human mitochondrial DNA is packaged with TFAM: D. Kang, et al. ; Nucleic Acids Res.
31, 1640 (2003),
Application(s): WB using human samples,
Abstract;
Studies characterizing 60 kda autoantibodies in subjects with schizophrenia: M.H. Rapaport, et al. ; Biol. Psychiatry
53, 361 (2003),
Application(s): WB using human samples,
Abstract;
Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients: K. Hara, et al. ; Clin. Exp. Immunol.
120, 285 (2000),
Application(s): WB using human samples,
Abstract;
Plasma membrane expression of heat shock protein 60 in vivo in response to infection: S.R. Carding, et al. ; Infect. Immun.
67, 4191 (1999),
Application(s): IHC using mouse samples,
Abstract;
Vaginal heat shock protein expression in symptom-free women with a history of recurrent vulvovaginitis: S.S. Witkin, et al. ; Am. J. Obstet. Gynecol.
180, 524 (1999),
Application(s): EIA using human samples,
Abstract;
Ehrlichia sennetsu groE operon and antigenic properties of the GroEL homolog: Y. Rikihisa, et al. ; FEMS Immunol. Med. Microbiol.
18, 39 (1997),
Application(s): WB using Ehrlichia samples,
Abstract;
Monoclonal antibodies raised against infectious haematopoietic necrosis virus (IHNV) G protein and a cellular 90 kDa protein neutralize IHNV infection in vitro: J.Y. Lee, et al.; J. Gen. Virol.
77, 1731 (1996),
Application(s): Western blot analysis compared to 90 kDa protein,
Abstract;
Full Text
The 60 kDa heat shock protein in human semen: relationship with antibodies to spermatozoa and Chlamydia trachomatis: S.S. Witkin, et al. ; Hum. Reprod.
11, 2600 (1996),
Application(s): EIA using human samples,
Abstract;
Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis: W. van Eden, et al. ; J. Exp. Med.
175, 1805 (1992),
Application(s): IHC using human samples,
Abstract;
