Product Details
| Alternative Name: | Chaperonin 60, CPN60, HspD1, heat shock protein 60 |
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| Clone: | LK-1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human Hsp60. |
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| UniProt ID: | P10809 |
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| GenBank ID: | M34664 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
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| Applications: | ELISA, Flow Cytometry, IF, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:100)
Immunoprecipitation (1:80)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~60kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor. |
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| Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or HSP60 antibody (LK-1) (right).
Immunohistochemistry analysis of human adrenal tissue stained with HSP60, mAb (LK-1) at 10µg/ml.
Western Blot Analysis of Hsp60 mAb (LK-1) (Prod. No. ADI-SPA-806): Lane 1: MW Marker, Lane 2: Hsp60 (human) (recombinant) Protein (Prod. No. ADI-NSP-540), Lane 3: HeLa Cell Lysate Heat Shocked (Prod. No. ADI-LYC-HL101), Lane 4: PC-12 Cell Lysate Heat Shocked (Prod. No. ADI-LYC-PC101), Lane 5: 3T3 Cell Lysate Heat Shocked (Prod. No. ADI-LYC-3T101)
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Product Literature References
MITOL promotes cell survival by degrading Parkin during mitophagy: I. Shiiba, et al.; EMBO Rep.
22, e49097 (2021),
Abstract;
Chronic heat stress regulates the relation between heat shock protein and immunity in broiler small intestine: F. Prosperi, et al.; Sci. Rep.
10, 18872 (2020),
Abstract;
Full Text
Impairment of mitochondrial unfolded protein response contribute to resistance declination of H2 O2 -induced injury in senescent MRC-5 cell model: T.Y. Lee, et al.; Kaohsiung J. Med. Sci.
36, 89 (2020),
Abstract;
Full Text
Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway: L. L'homme, et al.; Nutrients
12, 3771 (2020),
Abstract;
Full Text
The Step of Incorporation of Bacillus coagulans GBI-30 6086 Into “requeijão cremoso” Processed Cheese Does Not Affect Metabolic Homeostasis of Rats: M.B. Soares, et al.; Front. Microbiol.
10, 2332 (2019),
Abstract;
Full Text
Deletion of the neural tube defect-associated gene Mthfd1l disrupts one-carbon and central energy metabolism in mouse embryos: J.D. Bryant, et al.; J. Biol. Chem.
293, 5821 (2018),
Abstract;
Full Text
Impact of Trans-Fats on Heat-Shock Protein Expression and the Gut Microbiota Profile of Mice: G.C.B.C. Carvalho, et al.; J. Food Sci.
83, 489 (2018),
Abstract;
Control of tissue size and development by a regulatory element in the yorkie 3'UTR.: T. Umegawachi, et al.; Am. J. Cancer Res.
7, 673 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Modulatory effects of arginine, glutamine and branched-chain amino acids on heat shock proteins, immunity and antioxidant response in exercised rats: C.S. Moura, et al.; Food Funct.
8, 3228 (2017),
Abstract;
Neuroprotective effect of heat shock protein 60 on matrine-suppressed microglial activation: R. Zhang, et al.; Exp. Ther. Med.
14, 1832 (2017),
Abstract;
Full Text
Proteomic identification of proteins differentially expressed following overexpression of hTERT (human telomerase reverse transcriptase) in cancer cells: R.K. Jaiswal, et al.; Plos One
12, e0181027 (2017),
Application(s): WB in U2OS and HeLa cells,
Abstract;
Full Text
Antisense Oligonucleotide Mediated Splice Correction of a Deep Intronic Mutation in OPA1: T. Bonifert, et al.; Mol. Ther. Nucleic Acids
5, e390 (2016),
Abstract;
Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease: A. Kasza, et al.; J. Alzheimers Dis.
53, 557 (2016),
Abstract;
Full Text
Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium: L. Pelkonen, et al.; PLoS One
11, e0160352 (2016),
Application(s): Western blot,
Abstract;
Full Text
Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling: F. Ding, et al.; Biomed. Rep.
5, 623 (2016),
Application(s): Western Blot, BV2 cells,
Abstract;
Full Text
Proteomic Profiling of Neuroblastoma Cells Adhesion on Hyaluronic Acid-Based Surface for Neural Tissue Engineering: M.H. Yang, et al.; BioMed. Res. Int.
2016, 1917394 (2016),
Abstract;
Full Text
Cross-Omics Comparison of Stress Responses in Mesothelial Cells Exposed to Heat- versus Filter-Sterilized Peritoneal Dialysis Fluids: K. Katochwill, et al.; Biomed Res. Int.
2015, Article ID 628158 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Cytotoxic Effects of Tropodithietic Acid on Mammalian Clonal Cell Lines of Neuronal and Glial Origin: H. Wichmann, et al.; Mar. Drugs.
13, 7113 (2015),
Application(s): Indirect Immunofluorescence,
Abstract;
Full Text
Molecular Characterization and expression analysis of hsp60 gene homologue of Sheep Blowfly, Lucilia cuprina: M.K. Singh, et al.; J. Therm. Biol.
52, 24 (2015),
Application(s): Immunofluorescence, Western Blot,
Abstract;
Postexercise whole body heat stress additively enhances endurance training-induced mitochondrial adaptations in mouse skeletal muscle: Y. Tamura, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
307, R931 (2014),
Abstract;
Elevated heat shock protein 60 levels are associated with higher risk of coronary heart disease in Chinese: X. Zhang, et al.; Circulation
118, 2687 (2008),
Application(s): EIA using human plasma,
Abstract;
Full Text
Identification and characterization of the ER/lipid droplet-targeting sequence in 17β-hydroxysteroid dehydrogenase type 11: K. Motojima, et al. ; Arch. Biochem. Biophys.
479, 121 (2008),
Application(s): WB, IF using mouse cell lysates & tissue culture,
Abstract;
Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis: S.Y. Huang, et al.; Theriogenology
64, 1940 (2005),
Application(s): Immunohistochemistry on testis tissue,
Abstract;
Human MUC1 carcinoma-associated protein confers resistance to genotoxic anticancer agents: J. Ren, et al.; Cancer Cell.
5, 163 (2004),
Application(s): Immunoblot analysis,
Abstract;
Full Text
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