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HSP27 polyclonal antibody

 
ADI-SPA-803-D 50 µg 161.00 USD
 
ADI-SPA-803-F 200 µg 356.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:HspB1, Heat shock protein 27
 
Host:Rabbit
 
Immunogen:Recombinant human Hsp27.
 
UniProt ID:P04792
 
GenBank ID:L39370
 
Species reactivity:Human
Monkey, Porcine
 
Applications:ELISA, IHC, IP, WB
 
Recommended Dilutions/Conditions:ELISA (1:1,000)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~27kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
 
SPA-803 WB
Western blot analysis of HSP27 pAb: Lane 1: MW marker, Lane 2: HSP27 Recombinant Human Protein, Lane 3: HSP25 Recombinant Murine Protein (Negative Control), Lane 4: HeLa, Lane 5: HeLa, Heat Shocked, Lane 6: Vero, Heat Shocked, Lane 7: 3T3, Heat Shocked, Lane 8: PC-12, Heat Shocked.
SPA-803 IHC
Immunohistochemistry analysis of human heart tissue stained with HSP27, pAb at 10µg/ml.
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SPA-803 WB SPA-803 IHC

Product Literature References

Evaluation of geranylgeranylacetone against cisplatin-induced ototoxicity by auditory brainstem response, heat shock proteins and oxidative levels in guinea pigs: W.C. Lo, et al.; Neurotoxicol Teratol. 61, 29 (2017), Abstract;
SPARC Interacts with Actin in Skeletal Muscle in Vitro and in Vivo: L.H. Jørgensen, et al.; Am. J. Pathol. 187, 457 (2017), Abstract;
Expression of HSP27 in Hepatocellular Carcinoma: D. Eto, et al.; Anticancer Res. 36, 3775 (2016), Application(s): Immunohistochemical staining, hepactectomy tumor samples, Abstract; Full Text
Ovatodiolide Inhibits Breast Cancer Stem/Progenitor Cells through SMURF2-Mediated Downregulation of Hsp27: K.T. Lu, et al.; Toxins (Basel) 8, E127 (2016), Application(s): Western blot, Abstract; Full Text
Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin: T. Martins-Marques, et al.; J. Extracell. Vesicles 5, 32538 (2016), Application(s): Immunofluorescence staining and histology, tissue samples, Abstract; Full Text
Heat shock protein 27 promotes cell proliferation through activator protein-1 in lung cancer: S. Zhang, et al.; Oncol. Lett. 9, 2572 (2015), Abstract; Full Text
Acute response and subcellular movement of HSP27, αB-crystallin and HSP70 in human skeletal muscle after blood-flow-restricted low-load resistance exercise: K.T. Cumming, et al.; Acta Physiol. (Oxf) 211, 634 (2014), Abstract;
Methylglyoxal alters the function and stability of critical components of the protein quality control: C. F. Bento, et al.; PLoS One 5, e13007 (2010), Application(s): WB using human cell lysates, Abstract; Full Text
The small heat shock protein 27 is a key regulator of CD8+ CD57+ lymphocyte survival: K. Wood, et al.; J. Immunol. 15, 184 (2010), Application(s): EIA using human lymphocytes, Abstract; Full Text
Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay: K.S. Sinsimer, et al.; Mol. Cell Biol. 28, 5223 (2008), Abstract; Full Text
Chaperone hsp27, a novel subunit of AUF1 protein complexes, functionsin AU-rich element-mediated mRNA decay: G. Brewer, et al. ; Mol. Cell. Biol. 28, 5223 (2008), Application(s): WB using human cell lysates, Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): EIA using human vastus lateralis muscle tissue, Abstract; Full Text
Cell death inhibiting RNA (CDIR) derived from a 3'-untranslated region binds AUF1 and heat shock protein 27: L.P. Deiss, et al. ; J. Biol. Chem. 277, 47061 (2002), Application(s): WB using human samples, Abstract;
Overexpression of the human 72 kDa heat shock protein in renal tubular cells confers resistance against oxidative injury and cisplatin toxicity: Y. Tashima, et al. ; Nephrol. Dial. Transplant. 14, 1385 (1999), Application(s): WB using porcine samples, Abstract;

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