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HSP27 monoclonal antibody (G3.1)

 
ADI-SPA-800-D 50 µg 194.00 USD
 
ADI-SPA-800-F 200 µg 410.00 USD
 
ADI-SPA-800-J 1 mg 1,450.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-804-068

Product Specification

Alternative Name:HspB1, Heat shock protein 27
 
Clone:G3.1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Native human Hsp27.
 
UniProt ID:P04792
 
GenBank ID:L39370
 
Species reactivity:Human, Mouse, Rat
Bovine, Fish, Monkey
 
Applications:ELISA, ICC, IHC (PS), IP, WB
Electron microscopy, in vitro Assay
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~27kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
 
SPA-800 IHC
Immunohistochemistry analysis of human skeletal muscle tissue stained with HSP27, mAb (G3.1) at 10µg/ml.
ADI-SPA-800 WB
Western blot analysis of HSP27, mAb (G3.1) (Prod. No. ADI-SPA-800). Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: Vero (heat shocked), Lane 4: HSP27 (human) (recombinant) (Prod. No. ADI-SPP-715).
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SPA-800 IHC ADI-SPA-800 WB

Product Literature References

Conservative Mechanisms of Extracellular Trap Formation by Annelida Eisenia andrei: Serine Protease Activity Requirement: J. Homa, et al.; PLoS One 11, e0159031 (2016), Application(s): Immunostaining, Abstract; Full Text
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol. 79, 46 (2016), Application(s): Immunoblotting, Abstract;
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) Protein-Protein Interaction Inhibitor Reveals a Non-Catalytic Role for GAPDH Oligomerization in Cell Death: N. Qvit, et al.; J. Biol. Chem. 291, 13608 (2016), Application(s): SDS-PAGE, Abstract; Full Text
IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis: Y. Asano, et al.; Sci. Rep. 12, 19174 (2016), Application(s): Western blot, Abstract; Full Text
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med. (2015), Application(s): Western Blot, Full Text
Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines: L.H. Lash, et al.; Molecules 20, 10399 (2015), Application(s): Western Blot, Abstract; Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Cytotoxicity effects induced by Zearalenone metabolites, αZearalenol and β Zearalenol, on cultured Vero cells: Z. Ouanes-Ben Othmen, et al. ; Toxicology 252, 72 (2008), Application(s): WB using monkey cell lysates, Abstract;
HSP27 is involved in the pathogenesis of kidney tubulointerstitialfibrosis: A. Djamali, et al. ; Am. J. Physiol. Renal Physiol. 295, F707 (2008), Application(s): WB using rat tissue, Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): ELISA, WB using human vastus lateralis muscle tissue, Abstract; Full Text
Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability: A.D. Verin, et al. ; FASEB J. 16, 1064 (2002), Application(s): IP using bovine samples, Abstract;
Estrogenic activity of procymidone in primary cultured rainbow trout hepatocytes (Oncorhynchus mykiss): E. Chiesara, et al. ; Toxicol. In Vitro 16, 475 (2002), Application(s): WB using fish (trout) samples, Abstract;
Modulation of heat-shock protein 27 (Hsp27) anti-apoptotic activity by methylglyoxal modification: T. Tsuruo, et al. ; J. Biol. Chem. 277, 45770 (2002), Application(s): IP using human samples, Abstract;
Subcorneal colocalization of the small heat shock protein, hsp27, with keratins and proteins of the cornified cell envelope: F. Trautinger, et al. ; Br. J. Dermatol. 147, 13 (2002), Application(s): EM using human samples, Abstract;
Subcorneal colocalization of the small heat shock protein, hsp27, with keratins and proteins of the cornified cell envelope: F. Trautinger, et al. ; Br. J. Dermatol. 147, 13 (2002), Application(s): IHC using human samples, Abstract;
Effect of the serine protease inhibitor N-tosyl-L-phenylalanine-chloromethyl ketone (TPCK) on MCF-7 mammary tumour cells growth and differentiation: P. Galand, et al. ; Arch. Biochem. Biophys. 24, 153 (2000), Application(s): WB using mouse samples, Abstract;
Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors: I.J. Benjamin, et al. ; J. Biol. Chem. 271, 24089 (1996), Application(s): WB using rabbit samples, Abstract;

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