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HSP20 polyclonal antibody

ADI-SPA-796-D 50 µg 204.00 USD
ADI-SPA-796-F 200 µg 419.00 USD
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Product Specification

Alternative Name:HspB6, Heat shock protein 20
Immunogen:Rat skeletal muscle Hsp20.
UniProt ID:P97541
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Fish
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~20kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In 0.1M phosphate buffer, pH 7.0, containing 3% BSA and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Hsp20 is a member of the family of small heat shock proteins (sHsps), with homology to Hsp25, Hsp27, and alphaB-crystallin. Highest expression of Hsp20 is found in skeletal, smooth, and cardiac muscle. Under heat shock, Hsp20 translocates from the cytosol to the nucleus, although the amount of Hsp20 in the cell does not increase like other Hsps. Hsp20 oligomerization and interaction with polymeric actin filaments is regulated via phosphorylation by PKA and PKG.
Western blot analysis of HSP20, pAb (Prod. no. ADI-SPA-796): Lane 1: MW Marker, Lane 2: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101), Lane 3: 3T3 (heat shocked) (Prod. No. ADI-LYC-3T101), Lane 4: PC-12 (heat shocked) (Prod. No. ADI-LYC-PC101)
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Product Literature References

HSPB2 is dispensable for the cardiac hypertrophic response but reduces mitochondrial energetics following pressure overload in mice: T. Ishiwata, et al.; PLoS One 7, e42118 (2012), Application(s): WB using muscle samples, Abstract; Full Text

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Related Literature

Heat Shock Proteins & the Cellular Stress Response Catalog
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