Product Details
| Alternative Name: | HspA5, Glucose Regulated Protein 78, Hsp70-5, Heat shock protein 70 family |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of rat Grp78 (BiP). The sequence is completely conserved in mouse and hamster. |
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| UniProt ID: | P06761 |
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| GenBank ID: | M14050 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Mouse, Rat
Bovine, Fungus, Hamster, Monkey, Rabbit, Xenopus
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| Applications: | WB
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| Application Notes: | Detects a band of ~78kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Grp78 (Glucose Regulated 78kDa Protein), is also known as BiP or Immunoglobulin heavy chain binding protein. It is a stressresponse protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding, and assembly of many membrane bound and secreted proteins as well as for maintenance of cell homeostasis and the prevention of apoptosis. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Grp78, pAb (Prod. No. ADI-SPA-769): Lane 1: MW marker, Lane2: Grp78/BiP (hamster), (recombinant) (Prod. No. ADI-SPP-765), Lane3: Liver (rat), (microsome extract) (Prod. No. ADI-LYT-RM100)
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Product Literature References
CHIP Haploinsufficiency Exacerbates Hepatic Steatosis via Enhanced TXNIP Expression and Endoplasmic Reticulum Stress Responses: J.H. Han, et al.; Antioxidants
12, 458 (2023),
Abstract;
ERp29, an Endoplasmic Reticulum Secretion Factor Is Involved in the Growth of Breast Tumor Xenografts: S. Mkrtchian, et al. ; Mol. Carcinog.
47, 886 (2008),
Application(s): WB using human tissue culture,
Abstract;
Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med.
12, 2482 (2008),
Application(s): WB using rat cell lysates,
Abstract;
Small heat-shock proteins select DeltaF508-CFTR for endoplasmic reticulum-associated degradation: J. Brodsky, et al. ; Mol. Biol. Cell
18, 806 (2007),
Application(s): WB using human cell lysates,
Abstract;
Bax plays a pivotal role in thapsigargin-induced apoptosis of human colon cancer HCT116 cells by controlling Smac/Diablo and Omi/HtrA2 release from mitochondria: H.G. Wang, et al. ; Cancer Res.
63, 1483 (2003),
Application(s): WB using human samples,
Abstract;
gamma-Secretase activity requires the presenilin-dependent trafficking of nicastrin through the Golgi apparatus but not its complex glycosylation: B. De Strooper, et al. ; J. Cell. Sci.
116, 1127 (2003),
Application(s): ICC using mouse samples,
Abstract;
Mutant SOD1 linked to familial amyotrophic lateral sclerosis, but not wild-type SOD1, induces ER stress in COS7 cells and transgenic mice: T. Kato, et al. ; Biochem. Biophys. Res. Commun.
303, 496 (2003),
Application(s): ICC, WB, IHC using monkey samples,
Abstract;
Differential interaction of molecular chaperones with procollagen I and type IV collagen in corneal endothelial cells: E.P. Kay, et al. ; Mol. Vis.
8, 9 (2002),
Application(s): ICC using rabbit samples,
Abstract;
Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP: M. Mori, et al. ; J. Biol. Chem.
277, 12343 (2002),
Application(s): WB using mouse samples,
Abstract;
The protein kinase/endoribonuclease IRE1 alpha that signals the unfolded protein response has a luminal N-terminal ligand-independent dimerization domain: R.J. Kaufman, et al. ; J. Biol. Chem.
277, 18346 (2002),
Application(s): IP using human & monkey samples,
Abstract;
Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells: T.S. McClintock, et al. ; J. Neurochem.
72, 2301 (1999),
Application(s): ICC using human & xenopus samples,
Abstract;
General Literature References
The critical roles of endoplasmic reticulum chaperones and unfolded protein response in tumorigenesis and anticancer therapies: B. Luo & A.S. Lee; Oncogene
32, 805 (2013), (Review),
Abstract;
Full Text
The critical role of GRP78 in physiologic and pathologic stress: K.T. Pfaffenbach & A.S. Lee; Curr. Opin. Cell Biol.
23, 150 (2011), (Review),
Abstract;
Full Text
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