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HSC70/HSP70 polyclonal antibody

ADI-SPA-757-D 50 µg 143.00 USD
ADI-SPA-757-F 200 µg 352.00 USD
ADI-SPA-757-J 1 mg 1,300.00 USD
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Product Specification

Alternative Name:Heat shock protein 70, Hsc70, Hsp73
Immunogen:Synthetic peptide corresponding to a portion of rat Hsp70.
UniProt ID:P63018
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Monkey, Porcine, Plant, Rabbit, Sheep, Yeast
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~70kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Immunohistochemistry analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10µg/ml.
Western blot analysis of HSC70/HSP70, pAb (Product No. ADI-SPA-757): Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate (Product No. ADI-LYC-3T100), Lane 3: 3T3 Cell Lysate (heat shocked) (Product No. ADI-LYC-3T101), Lane 4: PC-12 Cell Lysate (Product No. ADI-LYC-PC100), Lane 5: PC-12 Cell Lysate (heat shocked) (Product No. ADI-LYC-PC101), Lane 6: HeLa Cell Lysate (Product No. ADI-LYC-HL100), Lane 7: HeLa Cell Lysate (heat shocked) (Product No. ADI-LYC-HL101), Lane 8: HSP70/HSP72 (human), (recombinant) (Product No. ADI-NSP-555), Lane 9: HSC70/HSP73 (bovine), (recombinant) (Product No. ADI-SPP-751)
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Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism: P.A. Erokhov, et al.; Oncotarget 8, 70695 (2017), Abstract; Full Text
Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeons: S. Lee, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 198, 87 (2016), Application(s): Used with the primary antibody, Abstract;
Proteomic identification of hair cell repair proteins in the model sea anemone Nematostella vectensis: P.C. Tang, et al.; Hear. Res. 327, 245 (2015), Application(s): Inhibition experiments, Abstract;

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