Product Details
Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to human calcineurin A. |
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UniProt ID: | P16298 |
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GenBank ID: | M29550 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat Bovine, Chicken, Dog, Drosophila, Hamster, Monkey, Porcine, Rabbit, Sheep
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Applications: | IP, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~61kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Calcineurin is a Ca2+/Calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit and a myristylated regulatory B subunit that binds calcium. Calcineurin, which is the target of immunosuppressant drugs such as cyclosporin A and FK506, controls the translocation of Nuclear factors of activated T-cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T-cells. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of ADI-SPA-610: Lane 1: Rat Brain Lysate, Lane 2: Mouse Brain Lysate.
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Product Literature References
Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation: A.B. Bachman, et al.; Nat. Commun.
9, 265 (2018),
Application(s): Immunoprecipitation,
Abstract;
Full Text
Matching of calcineurin activity to upstream effectors is critical for skeletal muscle fiber growth: R.N. Michel, et al. ; J. Cell Biol.
151, 663 (2000),
Application(s): WB using mouse samples,
Abstract;
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