Product Details
| Alternative Name: | CALR, CRT |
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| Clone: | FMC 75 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant calreticulin. |
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| UniProt ID: | P27797 (human) |
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| Source: | Purified from ascites |
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| Species reactivity: | Human
Monkey
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| Applications: | Flow Cytometry
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Predicted MW of ~63kDa. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. Protect from light. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | The multifunctional, multi -compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins, as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the solub le paralog of the ER membrane protein Calnexin (Cnx). Crt's three domains include a 180 residue N-terminal domain, a proline-rich Pdomainresidues 189 -288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P -domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N -glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol.
208, 1371 (2022),
Abstract;
IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer
20, 647 (2020),
Abstract;
Full Text
Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun.
9, 3194 (2018),
Abstract;
Full Text
The novel immunomodulator IMMUNEPOTENT CRP combined with chemotherapy agent increased the rate of immunogenic cell death and prevented melanoma growth: M.D.C. Rodriquez-Salazar, et al.; Oncol. Lett.
14, 844 (2017),
Abstract;
Full Text
Human CD1c(+) DCs are critical cellular mediators of immune responses induced by immunogenic cell death: S. Di Blasio, et al.; Oncoimmunology
5, e1192739 (2016),
Abstract;
Full Text
CD47 Agonist Peptides Induce Programmed Cell Death in Refractory Chronic Lymphocytic Leukemia B Cells via PLCγ1 Activation: Evidence from Mice and Humans: A.C. Martinez-Torres, et al.; PLoS Med.
12, e1001796 (2015),
Application(s): Flow Cytometry,
Abstract;
Full Text
Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis: Kuriyama T., et al.; Blood
120, 4058 (2012),
Application(s): Sorting and analysis of human acute promyelocytic leukemia cell line NB4 by flow cytometry,
Abstract;
Full Text
Immunogenic Tumor Cell Death Induced by Chemoradiotherapy in Patients with Esophageal Squamous Cell Carcinoma: Suzuki Y., et al.; Cancer Res.
72, 3967 (2012),
Application(s): Flow cytometry analysis of cell surface calreticulin expression in human esophageal squamous cell carcinoma (ESCC),
Abstract;
Full Text
Calreticulin Is the Dominant Pro-Phagocytic Signal on Multiple Human Cancers and Is Counterbalanced by CD47: Chao M. P., et al.; Science Translational Medicine
2, 63ra94 (2010),
Application(s): Flow cytometry analysis of human cell surface calreticulin expression,
Abstract;
Full Text
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