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Calreticulin monoclonal antibody (FMC 75)

 
ADI-SPA-601-D 50 µg 212.00 USD
 
ADI-SPA-601-F 200 µg 410.00 USD
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Product Specification

Alternative Name:CALR, CRT
 
Clone:FMC 75
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant calreticulin.
 
UniProt ID:P27797 (human)
 
Species reactivity:Human
Monkey
 
Applications:ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (12.5µg/ml)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Predicted MW of ~63kDa.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Calreticulin (CRT) is a multifunctional, multi-compartmental protein most abundant in the ER lumen. CRT contains the ER-retrieval sequence, KDEL, and has been best characterized as a soluble molecular chaperone of new or misfolded proteins and a Ca2+- binding protein. Both CRT and its membrane bound homolog, Calnexin (CNX) interact with proteins and glycoproteins that have monoglucosylated N-glycans. The CRT/CNX cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligimerization, regulates ER degradation, and provides quality control by preventing incompletely folded glycoproteins from exiting to the Golgi complex.
 
SPA-601 IHC
Immunohistochemistry analysis of human uterus tissue stained with Calreticulin, mAb (FMC 75) at 10µg/ml.
SPA-601 WB
Western blot analysis of Calreticulin: Lane 1: HeLa (Heat Shocked), Lane 2: Vero.
Please mouse over
SPA-601 IHC SPA-601 WB

Product Literature References

Cell density-induced changes in lipid composition and intracellular trafficking: S. Kavaliauskiene, et al.; Cell. Mol. Life Sci. 71, 1097 (2014), Abstract;
Induction of the Unfolded Protein Response Drives Enhanced Metabolism and Chemoresistance in Glioma Cells: L.M. Epple, et al.; PLoS One 8, e73267 (2013), Application(s): Immunohistochemistry using formalin-fixed, paraffin-embedded human glioma tumor or murine brain tissue samples and Western blot using human glioma tissue samples and cell lines, Abstract; Full Text
Proteinase 3, the Autoantigen in Granulomatosis with Polyangiitis, Associates with Calreticulin on Apoptotic Neutrophils, Impairs Macrophage Phagocytosis, and Promotes Inflammation: J. Gabillet, et al.; J. Immunol. 189, 2574 (2012), Application(s): Flow cytometry using human neutrophils, Abstract; Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Expression of the molecular chaperone Hsp70 in detergent-resistant microdomains correlates with its membrane delivery and release: M. Bachelet, et al. ; J. Biol. Chem. 278, 21601 (2003), Application(s): WB using human samples, Abstract;
Regulation by heavy metals and temperature of the human BAG-3 gene, a modulator of Hsp70 activity: A. Leone, et al. ; FEBS Lett. 541, 15 (2003), Application(s): ICC, WB using human samples, Abstract;
Identification and pharmacological correction of a membrane trafficking defect associated with a mutation in the sulfonylurea receptor causing familial hyperinsulinism: A. Sivaprasadarao, et al. ; J. Biol. Chem. 276, 35947 (2001), Application(s): ICC using human samples, Abstract;
Pharmacological chaperones rescue cell-surface expression and function of misfolded V2 vasopressin receptor mutants: M. Bouvier, et al. ; J. Clin. Invest. 105, 887 (2000), Application(s): ICC using monkey samples, Abstract;

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