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ERp57 polyclonal antibody

ADI-SPA-585-D 50 µg 164.00 USD
ADI-SPA-585-F 200 µg 363.00 USD
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Product Specification

Alternative Name:PDIA3, ERp60, ERp61, GRP57, Grp58
Immunogen:Native rat liver ERp57.
UniProt ID:P11598
Gene/Protein Identifier:NM_017319 (RefSeq)
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~57kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:ERp57 (also known as Grp58), a member of the Thioredoxin superfamily, is an ER protein with disulfide isomerase activity. It has been shown to increase after oncogenic transformation and to be a component of the MHC Class I peptide-loading complex.
SPA-585 WB
Western blot analysis of ERp57 (Grp58): Lane 1: MW marker, Lane 2: CHO-K1 lysate, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes.
Immunohistochemistry analysis of human colon tissue stained with ERp57, pAb at 10µg/ml.
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SPA-585 WB SPA-585 IHC

Product Literature References

Comparative proteome analysis of monolayer and spheroid culture of canine osteosarcoma cells: C. Gebhard, et al.; J. Proteomics (2018), Application(s):IHC, Abstract;
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration: V. Castillo, et al.; PLoS One 10, e0136620 (2015), Application(s): Densitometry analysis Western blot, Abstract; Full Text

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