Product Details
| Alternative Name: | MT1A, Metallothionein MGC32848, MT1S, MTC, metallothionein 1A (functional) |
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| Clone: | UC1MT |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Cross-linked rabbit liver Metallothionein I and II. |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Fish, Horse, Rabbit
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| Applications: | ELISA, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~15kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Prod. No. ADI-SPA-550: Lane 1: MW marker, Lane 2: MT I, Lane 3: MT II, Lane 4: Mummichug CdCl2.
Immunohistochemistry analysis of human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10µg/ml.
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Product Literature References
The protease DDI2 regulates NRF1 activation in response to cadmium toxicity: S.T. Ribeiro, et al.; iScience
25, 105227 (2022),
Abstract;
Morphofunctional Alterations in Zebrafish (Danio rerio) Gills after Exposure to Mercury Chloride.: R. Macirella & E. Brunelli; Int. J. Mol. Sci.
18, 824 (2017),
Abstract;
Full Text
Effects of Two Sublethal Concentrations of Mercury Chloride on the Morphology and Metallothionein Activity in the Liver of Zebrafish (Danio rerio): R. Macirella, et al.; Int. J. Mol. Sci.
17, e361 (2016),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Metallothioneins and heat shock proteins 70 in Armadillidium vulgare (Isopoda, Oniscidea) exposed to cadmium and lead: V. Mazzei, et al.; Ecotoxicol. Environ. Saf.
116, 99 (2015),
Application(s): Immunohistochemistry, Western Blotting ,
Abstract;
Identification and spatial analysis of metallothioneins expressed by the adult human lens: M. Kantorow, et al. ; Invest. Ophthalmol. Vis. Sci.
42, 188 (2001),
Application(s): WB using human samples,
Abstract;
Potentiation of cadmium-induced cytotoxicity by sulfur amino acid deprivation through activation of extracellular signal-regulated kinase1/2 (ERK1/2) in conjunction with p38 kinase or c-jun N-terminal kinase (JNK). Complete inhibition of the potentiated t: S.G. Kim, et al. ; Biochem. Pharmacol.
62, 1379 (2001),
Application(s): WB using rat samples,
Abstract;
Immunomodulatory activities of extracellular metallothionein. I. Metallothionein effects on antibody production: E.A. Olson, et al. ; Toxicology
85, 161 (1993),
Application(s): EIA (RB), WB using horse & rabbit samples,
Abstract;
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