[pSer⁸²]HSP27 polyclonal antibody



ADI-SPA-524-D	50 µl	225.00 USD

ADI-SPA-524-F	200 µl	458.00 USD
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Replaces Prod. #: ADI-SPA-524PU

Product Details

Alternative Name:	HspB1, Heat shock protein 27

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser⁸².

UniProt ID:	P04792

GenBank ID:	L39370

Source:	Whole rabbit serum.

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Dog, Guinea pig, Sheep

Applications:	IF, IP, WB

Recommended Dilutions/Conditions:	Immunoprecipitation (1:100) Western Blot (1:2,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~27kDa by Western blot.

Formulation:	Liquid. Whole rabbit serum.

Use/Stability:	Serum should be stored at -20^oC for long term storage. For short term storage, 4^oC, supplement with 0.1% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Regulatory Status:	RUO - Research Use Only

[pSer82]HSP27 polyclonal antibody Western blot

Western blot analysis of phosphorylated HSP27 and nonphosphorylated HSP27 proteins probed with HSP27 (pSer82) pAb

[pSer82]HSP27 polyclonal antibody Immunofluorescence

Immunofluorescent analysis (confocal) staining of HeLa cells using Hsp27 (pSer82) pAb (green); nuclei are stained in blue pseudocolor using DRAQ5

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Product Literature References

Release of Phosphorylated HSP27 (HSPB1) from Platelets Is Accompanied with the Acceleration of Aggregation in Diabetic Patients: H. Tokuda, et al.; PLoS One 10, e0128977 (2015), Application(s): Western Blot, Abstract; Full Text

Age-related differences in skeletal muscle insulin signaling: the role of stress kinases and heat shock proteins: P. Geiger, et al. ; J. Appl. Physiol. 105, 839 (2008), Application(s): WB using rat tissue, Abstract;

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[pSer82]HSP27 polyclonal antibody