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HSP40/Hdj1 polyclonal antibody

 
ADI-SPA-400-D 50 µg 140.00 USD
 
ADI-SPA-400-F 200 µg 334.00 USD
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Product Specification

Alternative Name:Heat shock protein 40
 
Host:Rabbit
 
Immunogen:Recombinant human Hsp40.
 
UniProt ID:P25685
 
GenBank ID:D49547
 
Species reactivity:Human, Mouse, Rat
Beluga, Bovine, Chicken, Dog, Fish, Guinea pig, Hamster, Monkey, Mussel, Porcine, Rabbit, Scallop, Sheep, Xenopus
 
Applications:ICC, IF, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~40kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hsp40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ, named after its homolog in E. coli. Hsp40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with Hsp70. Binding of Hsp40 to Hsp70 increases Hsp70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. coli). Repeated cycles of Hsp40/Hsp70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell.
 
Technical Info/Product Notes:Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000132002).
 
SPA-400 IHC
Immunohistochemistry analysis of human placenta tissue stained with HSP40/Hdj1, pAb at 10µg/ml.
ADI-SPA-400 WB
Western Blot analysis of HSP40/Hdj1, pAb (Prod. No. SPA-400): Lane 1: M W Marker, Lane 2: HSP40/Hdj1 (human), (recombinant) Protein (Prod. No. SPP-400), Lane 3: HeLa (heat shocked), (cell lysate) (Prod. No. LYC-HL101) , Lane 4: 3T3 (heat shocked), (cell lysate) (Prod. No. LYC-3T101), Lane 5: PC-12, (cell lysate) (Prod. No. LYC-PC100), Lane 6: DnaJ (E. coli), (recombinant) (His-tag) Protein (Prod. No. SPP-640)(Negative Control)
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SPA-400 IHC ADI-SPA-400 WB

Product Literature References

Evaluation of geranylgeranylacetone against cisplatin-induced ototoxicity by auditory brainstem response, heat shock proteins and oxidative levels in guinea pigs: W.C. Lo, et al.; Neurotoxicol Teratol. 61, 29 (2017), Abstract;
BIIB021, a synthetic Hsp90 inhibitor, induces mutant ataxin-1 degradation through the activation of heat shock factor 1: Y. Ding, et al.; Neuroscience 327, 20 (2016), Application(s): Western blot, Abstract;
IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis: Y. Asano, et al.; Sci. Rep. 12, 19174 (2016), Application(s): Western blot, Abstract; Full Text
Reversibility of neuropathology and motor deficits in an inducible mouse model for FXTAS: R.K. Hukema, et al.; Hum. Mol. Genet. 24, 4948 (2015), Application(s): Immunofluorescence in mouse cells, Abstract;
Huntingtin fragments and SOD1 mutants form soluble oligomers in the cell: Y.N. Park, et al.; PLoS One 7, e40329 (2012), Application(s): WB using N2A and HeLA cells, Abstract; Full Text
Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat ShockProteins and Viral Tumor Antigens: H. Kasamatsu, et al. ; J. Virol. 83, 37 (2008), Application(s): WB, IP, IHC using monkey cell lysates, Abstract;
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): WB, IF using rat tissue, Abstract;
Detection of novel intracellular α-synuclein oligomeric speciesby fluorescence lifetime imaging: P. McLean, et al. ; FASEB J. 20, 2050 (2006), Application(s): WB using human cell lysates, Abstract;
Hsp70 and Hsp40 improve neurite outgrowth and suppress intracytoplasmic aggregate formation in cultured neuronal cells expressing mutant SOD1: G. Sobue, et al. ; Brain Res. 949, 22 (2002), Application(s): ICC, WB using mouse samples, Abstract;
Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy: D.E. Merry, et al. ; Hum. Mol. Genet. 11, 515 (2002), Application(s): WB using mouse samples, Abstract;
Parkin accumulation in aggresomes due to proteasome impairment: M.M. Mouradian, et al. ; J. Biol. Chem. 277, 47870 (2002), Application(s): ICC using monkey samples, Abstract;
Geldanamycin activates a heat shock response and inhibits huntingtin aggregation in a cell culture model of Huntington's disease: E.E. Wanker, et al. ; Hum. Mol. Genet. 10, 1307 (2001), Application(s): ICC, WB using monkey samples, Abstract;
Polyglutamine length-dependent interaction of Hsp40 and Hsp70 family chaperones with truncated N-terminal huntingtin: their role in suppression of aggregation and cellular toxicity: N. Nukina, et al. ; Hum. Mol. Genet. 9, 2009 (2000), Application(s): ICC using mouse samples, Abstract;
The Hdj-2/Hsc70 chaperone pair facilitates early steps in CFTR biogenesis: D.M. Cyr, et al. ; EMBO J. 18, 1492 (1999), Application(s): IP, ICC using human samples, Abstract;

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