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αA/αB-Crystallin polyclonal antibody

 
ADI-SPA-224-D 50 µg 151.00 USD
 
ADI-SPA-224-F 200 µg 356.00 USD
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Product Specification

Alternative Name:CRYAA/CRYAB, HSPB4/HSPB5
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human αA- and αB-Crystallin.
 
UniProt ID:P02489 (alphaA-crystallin), P02511 (alphaB-crystallin)
 
GenBank ID:U05569 (alphaA-crystallin), M28638 (alphaB-crystallin)
 
Species reactivity:Human, Mouse
Bovine
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~20kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Alpha-crystallins composed of alpha A (~20 kDa) and alpha B (~20 kDa) subunits, are major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens and they are also found in a variety of other tissues. The two other crystallin families, beta and gamma, are homologous to each other but not to the alpha family or the sHsp's. Alpha-crystallins are also referred to as small heat shock proteins, since they are induced by increased temperature in a variety of organisms. The alpha-crystallins have sequence homology as well as structural and functional similarities with the small Hsp's such as Hsp25/27. Most small heat shock proteins have four common structural and functional features: (i) molecular weight between 12 and 43kDa; (ii) the formation of large oligomeric complexes composed of alphaA-crystallin, alphaB-crystallin and Hsp25/27; (iii) the moderately conserved alpha-crystallin domain in the central region of the protein; and (iv) molecular chaperone activity. The alpha-crystallin domain comprises approximately 90 residues, is bounded by variable N-terminal and C-terminal extensions and is involved in oligomer assembly. Oligomers can reach 800kDa or more and are dynamic, exhibiting subunit exchanges and organizational plasticity, possibly leading to functional diversity. Phosphorylation of serine residues occurs during development and in response to stress, and usually decreases oligomer size. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation, although productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.
 
SPA-224 WB
Western blot analysis: Lane 1: MWM, Lane 2: Prod. No. ADI-SPP-225, Lane 3: Prod. No. ADI-SPP-227, Lane 4: HeLa HS, Lane 5: Mouse Heart.
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SPA-224 WB

Product Literature References

Cell Cycle Arrest by Kynurenine in Lens Epithelial Cells: R. Nagaraj, et al. ; Invest. Ophthalmol. Vis. Sci. 49, 5466 (2008), Application(s): ICC using mouse tissue culture, Abstract;

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