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αB-Crystallin monoclonal antibody (1B6.1-3G4)

ADI-SPA-222-D 50 µg 198.00 USD
ADI-SPA-222-F 200 µg 418.00 USD
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Product Specification

Alternative Name:CRYAB, HSPB5
Immunogen:Bovine αB-crystallin.
UniProt ID:P02510
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken
Applications:ICC, IF, IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~20kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.
SPA-222 WB
Western blot analysis of alpha B Crystallin: Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.
Immunohistochemistry analysis of human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10µg/ml.
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SPA-222 WB SPA-222 IHC

Product Literature References

FOXO protects against age-progressive axonal degeneration: I. Hwang, et al.; Aging Cell 17, e12701 (2018), Abstract; Full Text
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci. 19, 178 (2018), Application(s): Western Blot, Abstract; Full Text
Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed: M. Gagaoua, et al.; Meat Sci. 145, 308 (2018), Abstract;
Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses: M. Gagaoua, et al.; Food Chem. 250, 245 (2018), Application(s): Reverse phase protein array, Abstract;
The p75NTR-mediated effect of nerve growth factor in L6C5 myogenic cells: A. de Perini, et al.; BMC Res Notes. 10, 686 (2017), Application(s): Western Blot, Abstract; Full Text
ERK-regulated αB-crystallin induction by matrix detachment inhibits anoikis and promotes lung metastasis in vivo: D. Malin, et al.; Oncogene 34, 5626 (2015), Application(s): Western Blotting, Immunohistochemistry, Abstract;
Expression of alpha B crystallin and BCL2 in patients with infiltrating ductal carcinoma: M. Kim, et al.; Int. J. Clin. Exp. Pathol. 8, 8842 (2015), Application(s): Immunohistochemical staining with human samples, Abstract; Full Text
Renal tumor with alpha b crystallin expression: M. Kim, et al.; Int. J. Clin. Exp. Pathol. 8, 9383 (2015), Application(s): Immunohistochemical staining with human samples, Full Text
Selective and Compartmentalised Myelin Expression of HspB5: S. Quraishe, et al.; Neuroscience 316, 130 (2015), Application(s): Electron Microscopy, Abstract;
High expression of crystallin αB represents an independent molecular marker for unfavourable ovarian cancer patient outcome and impairs TRAIL- and cisplatin-induced apoptosis in human ovarian cancer cells: J. Volkmann, et al.; Int. J. Cancer. 132, 2820 (2013), Application(s): WB and IHC using human ovarian cancer tissue samples, Abstract; Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Elevated retina-specific expression of the small heat shock protein,αA-crystallin, is associated with photoreceptor protection in experimental uveitis: N. Rao, et al. ; Invest. Ophthalmol. Vis. Sci. 49, 1161 (2008), Application(s): WB, IF using mouse cell lysates & tissue, Abstract;
Loop of Henle interaction with interstitial nodal spaces in the renalinner medulla: T. Pannabecker, et al. ; Am. J. Physiol. Renal Physiol. 295, F1744 (2008), Application(s): IHC using rat tissue, Abstract;
The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation: V.L. Cryns, et al. ; J. Biol. Chem. 276, 16059 (2001), Application(s): WB, ICC using human & mouse samples, Abstract;

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