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αB-Crystallin monoclonal antibody (1B6.1-3G4)

 
ADI-SPA-222-D 50 µg 194.00 USD
 
ADI-SPA-222-F 200 µg 410.00 USD
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Product Specification

Alternative Name:CRYAB, HSPB5
 
Clone:1B6.1-3G4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Bovine αB-crystallin.
 
UniProt ID:P02510
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken
 
Applications:ICC, IF, IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~20kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.
 
SPA-222 WB
Western blot analysis of alpha B Crystallin: Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.
SPA-222 IHC
Immunohistochemistry analysis of human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10µg/ml.
Please mouse over
SPA-222 WB SPA-222 IHC

Product Literature References

ERK-regulated αB-crystallin induction by matrix detachment inhibits anoikis and promotes lung metastasis in vivo: D. Malin, et al.; Oncogene 34, 5626 (2015), Application(s): Western Blotting, Immunohistochemistry, Abstract;
Expression of alpha B crystallin and BCL2 in patients with infiltrating ductal carcinoma: M. Kim, et al.; Int. J. Clin. Exp. Pathol. 8, 8842 (2015), Application(s): Immunohistochemical staining with human samples, Abstract; Full Text
Renal tumor with alpha b crystallin expression: M. Kim, et al.; Int. J. Clin. Exp. Pathol. 8, 9383 (2015), Application(s): Immunohistochemical staining with human samples, Full Text
Selective and Compartmentalised Myelin Expression of HspB5: S. Quraishe, et al.; Neuroscience 316, 130 (2015), Application(s): Electron Microscopy, Abstract;
High expression of crystallin αB represents an independent molecular marker for unfavourable ovarian cancer patient outcome and impairs TRAIL- and cisplatin-induced apoptosis in human ovarian cancer cells: J. Volkmann, et al.; Int. J. Cancer. 132, 2820 (2013), Application(s): WB and IHC using human ovarian cancer tissue samples, Abstract; Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;
Elevated retina-specific expression of the small heat shock protein,αA-crystallin, is associated with photoreceptor protection in experimental uveitis: N. Rao, et al. ; Invest. Ophthalmol. Vis. Sci. 49, 1161 (2008), Application(s): WB, IF using mouse cell lysates & tissue, Abstract;
Loop of Henle interaction with interstitial nodal spaces in the renalinner medulla: T. Pannabecker, et al. ; Am. J. Physiol. Renal Physiol. 295, F1744 (2008), Application(s): IHC using rat tissue, Abstract;
The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation: V.L. Cryns, et al. ; J. Biol. Chem. 276, 16059 (2001), Application(s): WB, ICC using human & mouse samples, Abstract;

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