Product Details
| Alternative Name: | CRYAB, HSPB5 |
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| Clone: | 1B6.1-3G4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Bovine αB-crystallin. |
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| UniProt ID: | P02510 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken
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| Applications: | ICC, IF, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~20kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10µg/ml.
Western Blot analysis of αB-Crystallin mAb (1B6.1-3G4) (Prod. No. ADI-SPA-222): Lane 1: MW Marker, Lane 2: α-Crystallin (bovine), (native) (Prod. No. ADI-SPP-225), Lane 3: αB-Crystallin (bovine), (native) (Prod. No. ADI-SPP-227), Lane 4: αA-Crystallin (bovine), (native) (Prod. No. ADI-SPP-226), Lane 5: β-Crystallin (bovine), (native) (Prod. No. ENZ-PRT224).
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Product Literature References
The small heat shock protein αB-Crystallin protects versus withaferin A-induced apoptosis and confers a more metastatic phenotype in cisplatin-resistant ovarian cancer cells: M.M. Carmichael, et al.; PLoS One
18, e0281009 (2023),
Abstract;
Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis: Z. Li, et al.; Int. J. Biol. Sci.
18, 2018 (2022),
Abstract;
Loss of FYCO1 leads to cataract formation: S. Kiyotoshi, et al.; Sci. Rep.
11, 13771 (2021),
Abstract;
Molecular Features of Lymph Node Metastasis in T1/2 Colorectal Cancer from Formalin-Fixed Paraffin-Embedded Archival Specimens: P. Steffen, et al.; J. Proteome Res.
20, 1304 (2021),
Abstract;
αB-crystallin response to a pro-oxidant non-cytotoxic environment in murine cardiac cells: An "in vitro" and "in vivo" study: A. Antonioni, et al.; Free Radic. Biol. Med.
152, 301 (2020),
Abstract;
CRYAB protects cardiomyocytes against heat stress by preventing caspase-mediated apoptosis and reducing F-actin aggregation: B. Yin, et al.; Cell Stress Chaperones
24, 59 (2019),
Abstract;
Eccentric training enhances the αB-crystallin binding to the myofibrils and prevents skeletal muscle weakness in adjuvant-induced arthritis rat: K. Himori, et al.; J. Appl. Physiol.
127, 71 (2019),
Abstract;
A knock-in/knock-out mouse model of HSPB8-associated distal hereditary motor neuropathy and myopathy reveals toxic gain-of-function of mutant Hspb8: D. Bouhy, et al.; Acta Neuropathol.
135, 131 (2018),
Abstract;
Full Text
FOXO protects against age-progressive axonal degeneration: I. Hwang, et al.; Aging Cell
17, e12701 (2018),
Abstract;
Full Text
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci.
19, 178 (2018),
Application(s): Western Blot,
Abstract;
Full Text
Preconditioning contractions prevent the delayed onset of myofibrillar dysfunction after damaging eccentric contractions: R. Yamada, et al.; J. Physiol.
596, 4427 (2018),
Abstract;
Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed: M. Gagaoua, et al.; Meat Sci.
145, 308 (2018),
Abstract;
Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses: M. Gagaoua, et al.; Food Chem.
250, 245 (2018),
Application(s): Reverse phase protein array,
Abstract;
The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise: J.M. Peake, et al.; J. Physiol.
595, 695 (2017),
Abstract;
Full Text
The p75NTR-mediated effect of nerve growth factor in L6C5 myogenic cells: A. de Perini, et al.; BMC Res Notes.
10, 686 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Association between cytoplasmic CRABP2, altered retinoic acid signaling, and poor prognosis in glioblastoma: R.Z. Liu, et al.; Glia
64, 963 (2016),
Abstract;
Full Text
Expression profiles of heat shock protein 27 and αB-crystallin and their effects on heat-stressed rat myocardial cells in vitro and in vivo: S. Tang, et al.; Mol. Med. Rep.
13, 1633 (2016),
Abstract;
HSP90 inhibitors in the context of heat shock and the unfolded protein response: effects on a primary canine pulmonary adenocarcinoma cell line: A.N. Graner, et al.; Int. J. Hyperthermia
20, 1 (2016),
Abstract;
Full Text
αB-Crystallin Interacts with Nav1.5 and Regulates Ubiquitination and Internalization of Cell Surface Nav1.5: Y. Huang, et al.; J. Biol. Chem.
291, 11030 (2016),
Abstract;
Full Text
ERK-regulated αB-crystallin induction by matrix detachment inhibits anoikis and promotes lung metastasis in vivo: D. Malin, et al.; Oncogene
34, 5626 (2015),
Application(s): Western Blotting, Immunohistochemistry,
Abstract;
Expression of alpha B crystallin and BCL2 in patients with infiltrating ductal carcinoma: M. Kim, et al.; Int. J. Clin. Exp. Pathol.
8, 8842 (2015),
Application(s): Immunohistochemical staining with human samples,
Abstract;
Full Text
Levels of oestrogen receptor, progesterone receptor and αB-crystallin in eutopic endometrium in relation to pregnancy in women with endometriosis: C. Moberg, et al.; Hum. Fertil.
18, 30 (2015),
Abstract;
Renal tumor with alpha b crystallin expression: M. Kim, et al.; Int. J. Clin. Exp. Pathol.
8, 9383 (2015),
Application(s): Immunohistochemical staining with human samples,
Full Text
αB-crystallin Expression in Breast Cancer is Associated with Brain Metastasis: K.D. Voduc, et al.; NPJ Breast Cancer
1, 15014 (2015),
Abstract;
Full Text
Acute response and subcellular movement of HSP27, αB-crystallin and HSP70 in human skeletal muscle after blood-flow-restricted low-load resistance exercise: K.T. Cumming, et al.; Acta Physiol. (Oxf.)
211, 634 (2014),
Abstract;
Comparative analysis of αB-crystallin expression in heat-stressed myocardial cells in vivo and in vitro: S. Tang, et al.; PLoS One
9, e86937 (2014),
Abstract;
Full Text
Effects of vitamin C and E supplementation on endogenous antioxidant systems and heat shock proteins in response to endurance training: K.T. Cumming, et al.; Physiol. Rep.
2, e12142 (2014),
Abstract;
Full Text
High expression of crystallin αB represents an independent molecular marker for unfavourable ovarian cancer patient outcome and impairs TRAIL- and cisplatin-induced apoptosis in human ovarian cancer cells: J. Volkmann, et al.; Int. J. Cancer.
132, 2820 (2013),
Application(s): WB and IHC using human ovarian cancer tissue samples,
Abstract;
Full Text
Influences of temperature, oxidative stress, and phosphorylation on binding of heat shock proteins in skeletal muscle fibers: N.T. Larkins, et al.; Am. J. Physiol. Cell. Physiol.
303, C654 (2012),
Application(s): Western-blot using rat skeletal muscles,
Abstract;
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell
134, 769 (2008),
Application(s): WB using human cell lysates,
Abstract;
Elevated retina-specific expression of the small heat shock protein,αA-crystallin, is associated with photoreceptor protection in experimental uveitis: N. Rao, et al. ; Invest. Ophthalmol. Vis. Sci.
49, 1161 (2008),
Application(s): WB, IF using mouse cell lysates & tissue,
Abstract;
Loop of Henle interaction with interstitial nodal spaces in the renalinner medulla: T. Pannabecker, et al. ; Am. J. Physiol. Renal Physiol.
295, F1744 (2008),
Application(s): IHC using rat tissue,
Abstract;
αB-crystallin promotes tumor angiogenesis by increasing vascular survival during tube morphogenesis: A. Dimberg, et al.; Blood
111, 2015 (2008),
Application(s): Western blot of bovine capillary endothelial (BCE) cell lysates,
Abstract;
The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation: V.L. Cryns, et al. ; J. Biol. Chem.
276, 16059 (2001),
Application(s): WB, ICC using human & mouse samples,
Abstract;
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