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GroES (E. coli) polyclonal antibody

 
ADI-SPA-210-D 50 µl 140.00 USD
 
ADI-SPA-210-F 200 µl 313.00 USD
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Product Specification

Alternative Name:Chaperonin 10
 
Host:Rabbit
 
Immunogen:E. coli GroES.
 
UniProt ID:P0A6F9 (strain K12)
 
GenBank ID:X07850
 
Species reactivity:E. coli
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:5,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~10kDa by Western blot.
 
Formulation:Liquid. Whole rabbit serum.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.
 
SPA-210 WB
Western blot analysis of GroES: Lane 1: MW marker, Lane 2: GroES Recombinant E. coli Protein, Lane 3: (Empty), Lane 4: E. coli Lysate.
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SPA-210 WB

Product Literature References

Prolyl isomerases in a minimal cell - catalysis of protein folding by trigger factor from Mycoplasma genitalium: A. Pahl, et al. ; Eur. J. Biochem. 267, 3270 (2000), Application(s): WB using bacteria (E. coli) samples, Abstract;
Expression of a gene encoding a 16.9-kDa heat-shock protein, Oshsp16.9, in Escherichia coli enhances thermotolerance: C.Y. Lin, et al. ; PNAS 94, 10967 (1997), Application(s): WB using bacteria (E. coli) samples, Abstract;

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