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Ubiquitin monoclonal antibody (P4G7-H11)

 
ADI-SPA-203-D 50 µg 205.00 USD
 
ADI-SPA-203-F 200 µg 399.00 USD
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Product Specification

Clone:P4G7-H11
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Native bovine Ubiquitin.
 
UniProt ID:P0CG53
 
Gene/Protein Identifier:NP_776558 (RefSeq)
 
Species reactivity:Human, Mouse, Rat
Bovine, Hamster, Rabbit
 
Applications:Flow Cytometry, IHC (PS), WB
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~6.5kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
 
SPA-203 FC 01
Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or Ubiquitin Monoclonal Antibody (P4G7-H11) (right) at 10µg/mL
SPA-203 IHC 01
Immunohistochemistry analysis of human brain, cortex tissue stained with Ubiquitin, mAb (P4G7-H11) at 5-10µg/ml.
SPA-203 WB
Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK
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SPA-203 FC 01 SPA-203 IHC 01 SPA-203 WB

Product Literature References

Lifespan differences between queens and workers are not explained by rates of molecular damage: E.R. Lucas, et al.; Exp. Gerontol. 92, 1 (2017), Abstract;
Distinct types of translation termination generate substrates for ribosome-associated quality control: N. Shcherbik, et al.; Nucleic Acids Res. 44, 6840 (2016), Application(s): Cell analysis of the modification of distinct ribosomal species, Abstract; Full Text
Transcriptional Induction of Metallothionein by Tris(pentafluorophenyl)stibane in Cultured Bovine Aortic Endothelial Cells: T. Fujie, et al.; Int. J. Mol. Sci. 17, 1381 (2016), Application(s): Western blot analysis of protein expression, Abstract; Full Text
ERK-dependent threonine phosphorylation of EGF receptor modulates receptor downregulation and signaling: S. Frank, et al. ; Cell. Signal. 20, 2145 (2008), Application(s): WB using hamster cell lysates, Abstract;

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