Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Ubiquitin polyclonal antibody

 
ADI-SPA-200-D 50 µg 161.00 USD
 
ADI-SPA-200-F 200 µg 356.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Host:Rabbit
 
Immunogen:Native bovine Ubiquitin.
 
UniProt ID:P0CG53
 
Gene/Protein Identifier:NP_776558 (RefSeq)
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus, Yeast
 
Applications:ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:5,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~8kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
 
SPA-200 WB
Western blot analysis: Ubiquitin protein probed with Ubiquitin pAb.
SPA-200 IHC
Immunohistochemistry analysis of human brain, cortex, senile plaque tissue stained with Ubiquitin, pAb at 10μg/ml.
Please mouse over
SPA-200 WB SPA-200 IHC

Product Literature References

Astaxanthin intake attenuates muscle atrophy caused by immobilization in rats: T. Shibaguchi, et al.; Physiol. Rep. 4, e12885 (2016), Application(s): SDS-PAGE and western blotting, Abstract; Full Text
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
SCA7 knockin mice model human SCA7 and reveal gradual accumulation of mutant ataxin-7 in neurons and abnormalities in short-term plasticity: H.Y. Zoghbi, et al. ; Neuron 37, 383 (2003), Application(s): ICC using mouse samples, Abstract;
von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination: M.F. Czyzyk-Krzeska, et al. ; PNAS 100, 2706 (2003), Application(s): WB using rat samples, Abstract;
p62 is a common component of cytoplasmic inclusions in protein aggregation diseases: H. Denk, et al. ; Am. J. Pathol. 160, 255 (2002), Application(s): IHC using mouse samples, Abstract;
Phosphorylation of retinoid X receptor suppresses its ubiquitination in human hepatocellular carcinoma: Y. Okano, et al. ; Hepatology 35, 332 (2002), Application(s): WB using human samples, Abstract;
HIV protease inhibitors protect apolipoprotein B from degradation by the proteasome: a potential mechanism for protease inhibitor-induced hyperlipidemia: S.L. Sturley, et al. ; Nat. Med. 7, 1327 (2001), Application(s): IP using human samples, Abstract;
Impairment of bile salt-dependent lipase secretion in AR4-2J rat pancreatic cells induces its degradation by the proteasome: N. Bruneau, et al. ; Biochim. Biophys. Acta 1530, 184 (2001), Application(s): WB using rat samples, Abstract;
Activation-dependent degradation of protein kinase C eta: C.S. Hahn, et al. ; Oncogene 19, 4263 (2000), Application(s): WB using human samples, Abstract;
The polyubiquitin gene is essential for meiosis in fission yeast: O. Niwa, et al. ; Exp. Cell Res. 254, 143 (2000), Application(s): WB using yeast samples, Abstract;
Tumor necrosis factor-alpha inhibits expression of CTP: phosphocholine cytidylyl transferase: S. Jackowski, et al. ; J. Biol. Chem. 275, 9699 (2000), Application(s): IP, WB using mouse samples, Abstract;
Ubiquitination of the human immunodeficiency virus type 1 Env glycoprotein: J. Haas, et al. ; J. Virol. 74, 5373 (2000), Application(s): IP using mouse samples, Abstract;
The degradation of apolipoprotein B100 is mediated by the ubiquitin-proteasome pathway and involves heat shock protein 70: H.N. Ginsberg, et al. ; J. Biol. Chem. 272, 20427 (1997), Application(s): IP using human samples, Abstract;

Related Products

HeLa (heat shocked) , (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL101-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

Brain (mouse), (tissue extract) 

Mouse brain extract., WB | Print as PDF
 
ADI-LYT-MB100-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

Goat anti-rabbit IgG, polyclonal antibody (HRP conjugate) 

ELISA, IHC, WB | Print as PDF
 
ADI-SAB-300-J 1 ml 118.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Ubiquitin
By biological activity:
Ubiquitin Polyclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,