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Ubiquitin polyclonal antibody

 
ADI-SPA-200-D 50 µg 169.00 USD
 
ADI-SPA-200-F 200 µg 330.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Host:Rabbit
 
Immunogen:Native bovine Ubiquitin.
 
UniProt ID:P0CG53
 
Gene/Protein Identifier:NP_776558 (RefSeq)
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus, Yeast
 
Applications:ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:5,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~8kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
 
Ubiquitin polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human brain, cortex, senile plaque tissue stained with Ubiquitin, pAb at 10μg/ml.
Ubiquitin polyclonal antibody Western blot
Western blot analysis: Ubiquitin protein probed with Ubiquitin pAb.
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Ubiquitin polyclonal antibody Immunohistochemistry Ubiquitin polyclonal antibody Western blot

Product Literature References

Correcting errors in the BRCA1 warning system: Y. Liang, et al.; DNA Repair 73, 120 (2018), Abstract;
Epidithiodiketopiperazines Inhibit Protein Degradation by Targeting Proteasome Deubiquitinase Rpn11: J. Li, et al.; Cell Chem. Biol. 25, 1350 (2018), Abstract;
PCB-126 effects on aryl hydrocarbon receptor, ubiquitin and p53 expression levels in a fish product (Sparus aurata L.): M. Calo, et al.; Nat. Prod. Res. 32, 1136 (2018), Abstract;
ARIH2 Ubiquitinates NLRP3 and Negatively Regulates NLRP3 Inflammasome Activation in Macrophages: A. Kawashima, et al.; J. Immunol. 199, 3614 (2017), Abstract;
Astaxanthin intake attenuates muscle atrophy caused by immobilization in rats: T. Shibaguchi, et al.; Physiol. Rep. 4, e12885 (2016), Application(s): SDS-PAGE and western blotting, Abstract; Full Text
TAX1BP1 Restrains Virus-Induced Apoptosis by Facilitating Itch-Mediated Degradation of the Mitochondrial Adaptor MAVS: Y.B. Choi, et al.; Mol. Cell. Biol. 37, e00422-16 (2016), Abstract; Full Text
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
SCA7 knockin mice model human SCA7 and reveal gradual accumulation of mutant ataxin-7 in neurons and abnormalities in short-term plasticity: H.Y. Zoghbi, et al. ; Neuron 37, 383 (2003), Application(s): ICC using mouse samples, Abstract;
von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination: M.F. Czyzyk-Krzeska, et al. ; PNAS 100, 2706 (2003), Application(s): WB using rat samples, Abstract;
p62 is a common component of cytoplasmic inclusions in protein aggregation diseases: H. Denk, et al. ; Am. J. Pathol. 160, 255 (2002), Application(s): IHC using mouse samples, Abstract;
Phosphorylation of retinoid X receptor suppresses its ubiquitination in human hepatocellular carcinoma: Y. Okano, et al. ; Hepatology 35, 332 (2002), Application(s): WB using human samples, Abstract;
HIV protease inhibitors protect apolipoprotein B from degradation by the proteasome: a potential mechanism for protease inhibitor-induced hyperlipidemia: S.L. Sturley, et al. ; Nat. Med. 7, 1327 (2001), Application(s): IP using human samples, Abstract;
Impairment of bile salt-dependent lipase secretion in AR4-2J rat pancreatic cells induces its degradation by the proteasome: N. Bruneau, et al. ; Biochim. Biophys. Acta 1530, 184 (2001), Application(s): WB using rat samples, Abstract;
Activation-dependent degradation of protein kinase C eta: C.S. Hahn, et al. ; Oncogene 19, 4263 (2000), Application(s): WB using human samples, Abstract;
The polyubiquitin gene is essential for meiosis in fission yeast: O. Niwa, et al. ; Exp. Cell Res. 254, 143 (2000), Application(s): WB using yeast samples, Abstract;
Tumor necrosis factor-alpha inhibits expression of CTP: phosphocholine cytidylyl transferase: S. Jackowski, et al. ; J. Biol. Chem. 275, 9699 (2000), Application(s): IP, WB using mouse samples, Abstract;
Ubiquitination of the human immunodeficiency virus type 1 Env glycoprotein: J. Haas, et al. ; J. Virol. 74, 5373 (2000), Application(s): IP using mouse samples, Abstract;
The degradation of apolipoprotein B100 is mediated by the ubiquitin-proteasome pathway and involves heat shock protein 70: H.N. Ginsberg, et al. ; J. Biol. Chem. 272, 20427 (1997), Application(s): IP using human samples, Abstract;

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