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Mn SOD (mouse), (recombinant) (His-tag)

 
ADI-SOD-200-D 50 µg 205.00 USD
 
ADI-SOD-200-F 200 µg 508.00 USD
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Product Specification

Alternative Name:Superoxide Dismutase, SOD2
 
Recommended Dilutions/Conditions:Western Blot (100ng protein, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~25kDa
 
Source:Produced in E. coli. Mouse Mn SOD is fused at the N-terminus to a His-tag.
 
UniProt ID:P09671
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 1.5 % glycerol and 80mM sodium chloride.
 
Purity:≥90% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
Activity assay
 
Application Notes:Activity Assay (superoxide dismutase). Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells: extracellular SOD (EC SOD, SOD-3); copper and zinc-containing SOD (Cu/Zn SOD, SOD-1); and manganese-containing SOD (Mn SOD, SOD-2). Mn SOD is an 80 kDa tetramer that contains Mn ions and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and modulation of the redox state of the mitochondria. Overexpression of this enzyme protects neurons from NMDA and nitric oxide-induced neurotoxicity.
 
SOD-200 SDS-PAGE
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 2µg, Lane 3: 5µg of Mn SOD (SOD2) Recombinant Mouse Protein.
SOD-200 WB
Western Blot analysis: Lane 1: MWM, Lane 2: Mouse Brain Tissue Extract, Lane 3: Recombinant Mn SOD Mouse Protein, probed with Mn SOD pAb (Prod. No. ADI-SOD-111).
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SOD-200 SDS-PAGE SOD-200 WB

Product Literature References

Endurance training increases the efficiency of rat skeletal muscle mitochondria: J.A. Zoladz, et al.; Pflugers Arch. 468, 1709 (2016), Application(s): Determination of protein levels through immunoblotting, Abstract; Full Text
Sod1 loss induces intrinsic superoxide accumulation leading to p53-mediated growth arrest and apoptosis: K. Watanabe, et al.; Int. J. Mol. Sci. 14, 10998 (2013), Application(s): Western blot, Abstract; Full Text

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