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Mn SOD polyclonal antibody

ADI-SOD-111-D 50 µg 238.00 USD
ADI-SOD-111-F 200 µg 441.00 USD
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Product Specification

Alternative Name:Manganese superoxide dismutase
Immunogen:Native rat Mn SOD.
UniProt ID:P07895
Gene/Protein Identifier:NP_058747 (RefSeq)
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Clam, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Applications:ELISA, Flow Cytometry, IHC (PS), IP, WB
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Immunoprecipitation (1:100)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~25kDa by Western blot.
Purity Detail:Immunoaffinity purified.
Formulation:Liquid. In sodium phosphate buffer, pH 7.0, containing 15mg/ml BSA, 0.09% sodium azide, and 50% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:SOD (Superoxide dismutase) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells, they are: EC SOD (extracellular SOD), Cu/Zn SOD (copper and zinc-containing SOD) and Mn SOD (manganese-containing SOD).
Western blot analysis of MnSOD pAb: Lane 1: HeLa Cell Lysate, Lane 2: Rat Brain Tissue Extract, Lane 3: Mouse Brain Tissue Extract
Immunohistochemistry analysis of human skeletal muscle tissue stained with Mn SOD, pAb at 10µg/ml.
Flow cytometry analysis of U251 cells using Mn SOD, pAb (right) and control rabbit IgG (left).
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Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Angiotensin receptor blockade improves cardiac mitochondrial activity in response to an acute glucose load in obese insulin resistant rats: M. Thorwald, et al.; Redox Biol. 14, 371 (2017), Abstract; Full Text
Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors: N.J. Yates, et al.; Neural Regen. Res. 12, 307 (2017), Abstract; Full Text
eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet: W.G. Choi, et al.; Nutr. 14, 48 (2017), Abstract; Full Text
Oncogenic MCT-1 activation promotes YY1-EGFR-MnSOD signaling and tumor progression: H.Y. Tseng, et al.; Oncogenesis 6, e313 (2017), Abstract; Full Text
Analysis of the Behavior of Mitochondria in the Ovaries of the Earthworm Dendrobaena veneta Rosa 1839: J. Faron, et al.; PLoS One 10, e0117187 (2015), Application(s): Immunofluorescence, Abstract; Full Text
Brain-Specific Superoxide Dismutase 2 Deficiency Causes Perinatal Death with Spongiform Encephalopathy in Mice: N. Izuo, et al.; Oxid. Med. Cell. Longev. 2015, Article ID 238914 (2015), Application(s): Western Blotting, Abstract; Full Text
NR2B-dependent cyclophilin D translocation suppresses the recovery of synaptic transmission after oxygen-glucose deprivation: Z. Zhang, et al.; Biochim. Biophys. Acta 1852, 2225 (2015), Application(s): Western blot , Abstract;
Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions: J.A. Collins, et al.; Free Radic. Biol. Med. 86, 57 (2015), Application(s): Western Blot, Abstract;
Scheme of Ischaemia-triggered Agents during Brain Infarct Evolution in a Rat Model of Permanent Focal Ischaemia: P. Bonova, et al.; J. Mol. Neurosci. 57, 73 (2015), Application(s): Western Blot, Abstract;
Effect of exposure to benzo[a]pyrene on SODs, CYP1A1/1A2- and CYP2E1 immunopositive proteins in the blood clam Scapharca inaequivalvis: M. Monari, et al. ; Mar. Environ. Res. 63, 200 (2007), Application(s): WB using clam tissue, Abstract;
Model mice for tissue-specific deletion of the manganese superoxide dismutase (MnSOD) gene: T. Shirasawa, et al. ; Biochem. Biophys. Res. Commun. 296, 729 (2002), Application(s): WB using mouse samples, Abstract;
Superoxide dismutase activity in organotypic midbrain-striatum co-cultures is associated with resistance of dopaminergic neurons to excitotoxicity: A. Akaike, et al. ; J. Neurochem. 76, 1336 (2001), Application(s): WB using rat samples, Abstract;
Garlic ameliorates gentamicin nephrotoxicity: relation to antioxidant enzymes: M.E. Ibarra-Rubio, et al. ; Free Radic. Biol. Med. 29, 602 (2000), Application(s): WB using rat samples, Abstract;

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