Product Details
| Alternative Name: | Manganese superoxide dismutase |
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| Host: | Rabbit |
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| Immunogen: | Human Mn SOD protein. |
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| UniProt ID: | P04179 |
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| Gene/Protein Identifier: | NP_000627 (RefSeq) |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Rabbit, Sheep, Xenopus
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~25kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | SOD (Superoxide dismutase) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells, they are: EC SOD (extracellular SOD), Cu/Zn SOD (copper and zinc-containing SOD) and Mn SOD (manganese-containing SOD). |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human skeletal muscle tissue stained with Mn SOD, pAb at 10µg/ml.
Western blot analysis of Mn SOD: Lane 1: MW Marker, Lane 2: HeLa Cell Lysate, Lane 3: Mouse Brain Tissue Extract, Lane 4: Rat Brain Tissue Extract
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Product Literature References
Daily blueberry consumption for 12 weeks improves endothelial function in postmenopausal women with above-normal blood pressure through reductions in oxidative stress: a randomized controlled trial: E.K. Woolf, et al.; Food Funct.
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A three-organelle complex made by wrappER contacts with peroxisomes and mitochondria responds to liver lipid flux changes: N. Ilacqua, et al.; J. Cell Sci.
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Abstract;
Autophagy displays divergent roles during intermittent amino acid starvation and toxic stress-induced senescence in cultured skeletal muscle cells: D. Bloemberg & J. Quadilatero; J. Cell. Physiol.
236, 3099 (2021),
Application(s): Western blot on mouse skeletal myoblasts (C2C12) lysates,
Abstract;
Repetitively Hypoxic Preconditioning Attenuates Ischemia/Reperfusion-Induced Liver Dysfunction Through Upregulation of Hypoxia-Induced factor-1 Alpha-Dependent Mitochondrial Bcl-xl in Rat: P. Chou, et al.; Chin. J. Physiol.
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Early induction of senescence and immortalization in PGC-1α-deficient mouse embryonic fibroblasts: I. Prieto, et al.; Free Radic. Biol. Med.
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A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
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Cellular Uptake and Bioavailability of Tocotrienol-Rich Fraction in SIRT1-Inhibited Human Diploid Fibroblasts: D. Torralba, et al.; Nat. Commun.
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Dynamic Phosphorylation of the C Terminus of Hsp70 Regulates the Mitochondrial Import of SOD2 and Redox Balance: S. Zemanovic, et al.; Cell Rep.
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Impact of carbonylation on glutathione peroxidase-1 activity in human hyperglycemic endothelial cells: C.S. Sultan, et al.; Redox Biol.
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Linagliptin unmasks specific antioxidant pathways protective against albuminuria and kidney hypertrophy in a mouse model of diabetes: N.Y. Spencer, et al.; PLoS One
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Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts: D. Torralba, et al.; Nat. Commun.
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Selective eradication of cancer displaying hyperactive Akt by exploiting the 3 metabolic consequences of Akt activation: V. Nogueira, et al.; eLife
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Effects of Conjugated Linoleic Acid Associated With Endurance Exercise on Muscle Fibres and Peroxisome Proliferator-Activated Receptor γ Coactivator 1 α Isoforms: R. Barone, et al.; J. Cell. Physiol.
232, 1086 (2017),
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Growth hormone secretagogues hexarelin and JMV2894 protectskeletal muscle from mitochondrial damages in a rat model of cisplatin-induced cachexia: G. Sirago, et al.; Sci. Rep.
7, 13017 (2017),
Abstract;
Full Text
Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice: S.A. Bloomer, et al.; Lipids Health Dis.
16, 243 (2017),
Abstract;
Full Text
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones
22, 225 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Antioxidant supplementation accelerates cachexia development by promoting tumor growth in C26 tumor-bearing mice: M. Assi, et al.; Free Radic. Biol. Med.
91, 204 (2016),
Application(s): Western blot,
Abstract;
Cerebrovascular endothelial dysfunction induced by mercury exposure at low concentrations: G.A. Wiggers, et al.; Neurotoxicology
53, 282 (2016),
Application(s): Western blot,
Abstract;
Effect of mitochondrial fission inhibition on C2C12 differentiation: D. Bloemberg, et al.; Data Brief
7, 634 (2016),
Application(s): Immunoblotting,
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Full Text
MDM4/HIPK2/p53 cytoplasmic assembly uncovers coordinated repression of molecules with anti-apoptotic activity during early DNA damage response: F. Mancini, et al.; Oncogene
35, 228 (2016),
Application(s): Western Blot,
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Full Text
Resistance training and redox homeostasis: Correlation with age-associated genomic changes: I. Dimauro, et al.; Redox Biol.
10, 34 (2016),
Application(s): Western blot, PBMCs,
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Full Text
The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells: I. Broniarek, et al.; Pflugers Arch.
468, 1541 (2016),
Application(s): Determination of protein levels via immunoblotting,
Abstract;
The effect of R-(-)-deprenyl administration on antioxidant enzymes in rat testis: F. Horvathova, et al. ; Eur. J. Pharmacol.
788, 21 (2016),
Application(s): Immunohistochemistry,
Abstract;
Alcohol and High Fat Induced Chronic Pancreatitis: TRPV4 Antagonist Reduces Hypersensitivity: L. Zhang, et al.; Neuroscience
311, 166 (2015),
Application(s): Immunohistochemical localization of MnSOD in pancreas tissue,
Abstract;
Cytotoxic and genotoxic effects mediated by M2 muscarinic receptor activation in human glioblastoma cells: M. Di Bari, et al.; Neurochem. Int.
90, 261 (2015),
Application(s): Western Blot,
Abstract;
Effects of deletion of the transcription factor Nrf2 and benzo [a]pyrene treatment on ovarian follicles and ovarian surface epithelial cells in mice: J. Lim, et al.; Reprod. Toxicol.
58, 24 (2015),
Application(s): Immunoblotting for SOD2 in ovarian extracts ,
Abstract;
Effects of Exercise and Alpha-Lipoic Acid Supplementation on Brain Tissue Protection in Experimental Diabetes: Z. Pündük; (2015), (PhD thesis),
Application(s): Western assay,
Full Text
The beneficial effects of exercise on cartilage are lost in mice with reduced levels of ECSOD in tissues: K.M. Pate, et al.; J. Appl. Physiol. (1985)
118, 760 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Glutathione depletion in antioxidant defense of differentiated NT2-LHON cybrids: E. Kirches, et al. ; Neurobiol. Dis.
25, 536 (2007),
Application(s): WB using human cell lysates,
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The role of peroxisomal ABC transporters in the mouse adrenal gland:the loss of Abcd2 (ALDR), Not Abcd1 (ALD), causes oxidative damage: J. Powers, et al. ; Lab. Invest.
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Application(s): IHC using mouse tissue,
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TLR4-mediated survival of macrophages is MyD88 dependent and requires TNF-α autocrine signalling: E. Lombardo, et al. ; J. Immunol.
178, 3731 (2007),
Application(s): WB using mouse cell lysates,
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Time sequence of maturation of dystrophic neurites associated with Abeta deposits in APP/PS1 transgenic mice: L. Pradier, et al. ; Exp. Neurol.
184, 247 (2003),
Application(s): IHC using mouse samples,
Abstract;
Heme oxygenase-1-derived carbon monoxide requires the activation of transcription factor NF-kappa B to protect endothelial cells from tumor necrosis factor-alpha -mediated apoptosis: M.P. Soares, et al. ; J. Biol. Chem.
277, 17950 (2002),
Application(s): WB using human & bovine samples,
Abstract;
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