Product Specification
| Clone: | MPC-11 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Species reactivity: | Species independent
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| Applications: | Flow Cytometry
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Isotype matched controls are useful in flow cytometry to determine a gross estimate of non-specific binding that may be observed with a specific antibody of interest. In general, an isotype control should be of the same species and isotype as the antibody of interest, used at the same concentration, and conjugated to the same fluorophore. This reagent is an IgG2b isotype mouse monoclonal antibody (Clone # MPC-11), conjugated to FITC. It is intended for use as an isotype control antibody for flow cytometry. |
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Flow cytometry analysis of 106 Jurkat cells using the Isotype Control for IgG2b FITC (Prod. No. ADI-SAB-602FI) compared with Heme Oxygenase-1, mAb (Prod. No. ADI-OSA-111FI), at a concentration of 50µg/ml
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General Literature References
Dermal infiltrates of cutaneous T-cell lymphomas with epidermotropism but not other cutaneous lymphomas are abundant with langerin+ dendritic cells: M. Der-Petrossian, et al.; J. Eur. Acad. Dermatol. Venereol.
25, 922 (2011),
Application(s): Microscopy of human T cells,
Abstract;
Isolation of Hofbauer Cells from Human Term Placentas with High Yield and Purity: Z. Tang, et al.; Am. J. Reprod. Immunol.
66, 336 (2011),
Application(s): Flow cytometry of human placenta cells,
Abstract;
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PD-1 expression on HIV-specific T cells is associated with T-cell exhaustion and disease progression: C.L. Day, et al.; Nature
443, 350 (2006),
Application(s): Flow cytometry of human blood samples,
Abstract;
Murine respiratory tract dendritic cells: isolation, phenotyping and functional studies: C.J. Calder, et al.; J. Immunol. Methods
287, 67 (2004),
Application(s): Flow cytometry of mouse dendritic cells,
Abstract;
Human dendritic cells express the thrombopoietin receptor, c-Mpl: T. Kumamoto, et al.; Br. J. Haematol.
105, 1025 (1999),
Application(s): FLOW, IC of human blood,
Abstract;
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