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Mouse IgG1 isotype control, monoclonal antibody (MOPC-21) (FITC conjugate)

ADI-SAB-600FI-050 50 µg 204.00 USD
ADI-SAB-600FI-200 200 µg 431.00 USD
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Product Specification

Species reactivity:Species independent
Applications:Flow Cytometry
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles. Protect from light.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Isotype matched controls are useful in flow cytometry to determine a gross estimate of non-specific binding that may be observed with a specific antibody of interest. In general, an isotype control should be of the same species and isotype as the antibody of interest, used at the same concentration, and conjugated to the same fluorophore. This reagent is an IgG1 isotype mouse monoclonal antibody (Clone # MOPC-21), conjugated to FITC. It is intended for use as an isotype control antibody for flow cytometry.
Flow cytometry analysis of 106 Jurkat cells using the Isotype Control for IgG1 FITC (Prod. No. ADI-SAB-600FI) compared with Hsp27, mAb (Prod. No. ADI-SPA-800FI), at a concentration of 50µg/ml.
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Product Literature References

The CD24hi smooth muscle subpopulation is the predominant fraction in uterine fibroids: M. Drosch, et al.; Mol. Hum. Reprod. 20, 664 (2014), Abstract;
Vasculogenic Conditioning of Peripheral Blood Mononuclear Cells Promotes Endothelial Progenitor Cell Expansion and Phenotype Transition of Anti-Inflammatory Macrophage and T Lymphocyte to Cells With Regenerative Potential: H. Masuda, et al.; JAHA 3, e000743 (2014), Application(s): Flow cytometry analysis of human peripheral blood mononuclear cells (PBMNCs), Abstract; Full Text
Altered expression of TNF-alpha signaling pathway proteins in systemic lupus erythematosus: L.J. Zhu, et al.; J. Rheumatol. 37, 1658 (2010), Abstract;
Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage: W.K. Kim, et al.; J. Leukoc. Biol. 87, 557 (2010), Application(s): Flow cytometry analysis of rhesus macaques peripheral blood monocytes (PBMCs), Abstract; Full Text
Expanded Population of Activated Antigen-Engaged Cells within the Naive B Cell Compartment of Patients with Systemic Lupus Erythematosus: N.H. Chang, et al.; J. Immunol. 180, 1276 (2008), Application(s): Flow cytometry analysis of human peripheral blood monocytes (PBMCs), Abstract; Full Text
Inhibition of Invariant Chain Processing, Antigen-Induced Proliferative Responses, and the Development of Collagen-Induced Arthritis and Experimental Autoimmune Encephalomyelitis by a Small Molecule Cysteine Protease Inhibitor: P.L. Podolin, et al.; J. Immunol. 180, 7989 (2008), Application(s): Flow cytometry analysis of human B cell line Raji, Abstract; Full Text
The effects of dasatinib on IgE receptor-dependent activation and histamine release in human basophils: M. Kneidinger, et al.; Blood 111, 3097 (2008), Abstract; Full Text
Nonfucosylated therapeutic IgG1 antibody can evade the inhibitory effect of serum immunoglobulin G on antibody-dependent cellular cytotoxicity through its high binding to FcγRIIIa.: S. Iida, et al.; Clin. Cancer Res. 12, 2879 (2006), Application(s): Flow cytometry analysis of human heparinized peripheral blood, Abstract; Full Text
Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking: X. Chen, et al.; Blood 106, 2409 (2005), Application(s): Flow cytometry analysis of human monocytes, Abstract; Full Text
Primary Antibody-Fab Fragment Complexes: A Flexible Alternative to Traditional Direct and Indirect Immunolabeling Techniques: J.K. Brown, et al.; J. Histochem. Cytochem. 52, 1219 (2004), Application(s): IHC on frozen murine jejunum tissue samples, Abstract;
Cytokine Control of Memory B Cell Homing Machinery: M.P. Roy, et al.; J. Immunol. 169, 1676 (2002), Application(s): Flow cytometry analysis of human germinal center B-cells, Abstract; Full Text
Expression of cyclin B1 and cyclin dependent kinase inhibitor p21 in lymphocytes in patients with systemic lupus erythematosus: C.Y. Ho, et al.; J. Rheumatol. 29, 2537 (2002), Abstract;
CD99 Signals Caspase-Independent T Cell Death: R.D. Pettersen, et al.; J. Immunol. 166, 4931 (2001), Abstract; Full Text
Preconditioning with fetal cord blood facilitates engraftment of primary childhood T-cell acute lymphoblastic leukemia in immunodeficient mice: D.P. Dialynas, et al.; Blood 97, 3218 (2001), Application(s): Flow cytometry analysis of human childhood T-lineage acute lymphoblastic leukemia (ALL) cells, Abstract; Full Text
Antigen contacts by Ni-reactive TCR: typical alphass chain cooperation versus alpha chain-dominated specificity: J. Vollmer, et al.; Int. Immunol. 12, 1723 (2000), Application(s): Flow cytometry analysis of human T- and B-cells, Abstract; Full Text
Effect of renal dialysis therapy modality on T cell cytokine production: A. Zamauskaite, et al.; Nephrol. Dial. Transplant. 14, 49 (1999), Application(s): Flow cytometry analysis of human heparinized blood samples, Abstract; Full Text
TCR Reactivity in Human Nickel Allergy Indicates Contacts with Complementarity-Determining Region 3 but Excludes Superantigen-Like Recognition: J. Vollmer, et al.; J. Immunol. 163, 2723 (1999), Application(s): Flow cytometry analysis of human T- and B-cells, Abstract; Full Text

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