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Green Fluorescent Protein monoclonal antibody (1E4)

 
ADI-SAB-500-E 100 µg 281.00 USD
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Product Specification

Alternative Name:GFP
 
Clone:1E4
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant GFP protein (Aequorea victoria).
 
UniProt ID:P42212
 
Gene/Protein Identifier:JQ1514 (PIR)
 
Species reactivity:Species independent
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (2µg/ml)
Immunohistochemistry (10µg/ml)
Immunoprecipitation (5µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~27kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The green fluorescent protein (GFP) is derived from the jellyfish Aequorea victoria, that fluoresces green when exposed to blue light. GFP is a frequently used reporter for gene expression in recombinant DNA and cell transfection systems.
 

Product Literature References

Myofibril growth during cardiac hypertrophy is regulated through dual phosphorylation and acetylation of the actin capping protein CapZ: Y.H. Lin, et al.; Cell. Signal. 28, 1015 (2016), Application(s): Co-Immunoprecipitation, Abstract;
Efficient cross-presentation depends on autophagy in tumor cells: H. Hu, et al. ; Cancer Res. 68, 6889 (2008), Application(s): IP, WB using human cell lysates, Abstract;

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