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FLAG, pAb

 
ADI-SAB-410-0100 100 µg 200.00 USD
 

Product Specification

Host:Rabbit
 
Isotype:IgG
 
Immunogen:Synthetic peptide (CDYKDDDDK) coupled to KLH.
 
Species reactivity:Species independent
 
Specificity:Recognizes FLAG-tagged proteins.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Long Term Storage:-20°C
 
Miscellaneous/General:FLAG-tag stands for an octapeptide with a sequence of DYKDDDDK. It is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It is widely used for affinity chromatography to purify recombinant proteins from variety of systems. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence, such as in ELISA, Western Blot, etc.
 
Background / Technical Information:FLAG is a registered trademark of Sigma-Aldrich Co. LLC
 
ADI-SAB-410 WB
Western blot analysis of anti-FLAG, pAb (Prod. No. ADI-SAB-410): Lane 1: MW marker, Lane 2: SuperFasLigand™ (soluble) (human), (recombinant) (Prod. No. ALX-522-020), Lane 3: MW marker, Lane 4: SuperFasLigand™ (soluble) (human), (recombinant) (Prod. No. ALX-522-020).
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ADI-SAB-410 WB

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