Product Specification
| Host: | Goat |
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| Immunogen: | Purified rabbit IgG (whole molecule). |
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| Species reactivity: | Rabbit
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| Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. |
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| Applications: | ELISA, IHC, WB
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| Recommended Dilutions/Conditions: | ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500, Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Immunoaffinity purified. |
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| Quality Control: | The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species. |
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| Formulation: | Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA (IgG-free, protease-free) and 50% glycerol. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG. |
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Product Literature References
AGR2 is a target of canonical Wnt/β-catenin signaling and is important for stemness maintenance in colorectal cancer stem cells: B. Dahal Lamichane, et al.; Biochem. Biophys. Res. Commun.
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Essential oil from Korean Chamaecyparis obtusa leaf ameliorates respiratory activity in Sprague‑Dawley rats and exhibits protection from NF-κB-induced inflammation in WI38 fibroblast cells: S. Raha, et al.; Int. J. Mol. Med.
43, 393 (2019),
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Perivascular Cells and NADPH Oxidase Inhibition Partially Restore Hyperglycemia-Induced Alterations in Hematopoietic Stem Cell and Myeloid-Derived Suppressor Cell Populations in the Bone Marrow: J.Y. Kim, et al.; Int. J. Stem Cells
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Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate: E. A. Park, et al.; Korean J. Parasitol.
57, 201 (2019),
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Rap1 regulates hepatic stellate cell migration through the modulation of RhoA activity in response to TGF-β1: M.Y. Moon, et al.; Int. J. Mol. Med.
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Rosuvastatin attenuates bioprosthetic heart valve calcification: S. Lee, et al.; J. Thorac. Cardiovasc. Surg.
158, 731 (2019),
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Set7 Is a H3K37 Methyltransferase in Schizosaccharomyces pombe and Is Required for Proper Gametogenesis: Y. Shen, et al.; Structure
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The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome: M.C. Trotta, et al.; PLoS One
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Transgenic overexpression of human LY6K in mice suppresses mature T cell development in the thymus: D. Son, et al.; Oncol. Lett.
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Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse: S. Wang, et al.; Biomaterials
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An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAK/NF-κB Signaling Pathways: J.Y. Lee, et al.; Front. Aging Neurosci.
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Anticancer and radiosensitizing effects of the cyclin-dependent kinase inhibitors, AT7519 and SNS‑032, on cervical cancer: M.S. Kang, et al.; Int. J. Oncol.
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Combined therapy involving electroacupuncture and treadmillexercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp Neurol.
300, 222 (2018),
Application(s): Western Blot,
Abstract;
Defensive Properties of Ginsenoside Re against UV-B-Induced Oxidative Stress through Up-Regulating Glutathione and Superoxide Dismutase in HaCaT Keratinocytes: D. Shin, et al.; Iran. J. Pharm. Res.
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Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer: C.H. Choi, et al.; Sci. Rep.
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Inhibition of aldose-reductase-2 by a benzofuroxane derivative bf-5m increases the expression of kcne1, kcnq1 in high glucose cultured H9c2 cardiac cells and sudden cardiac death: M.C. Trotta, et al.; Oncotarget
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Abstract;
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Osteogenic potential of cell spheroids composed of varying ratios of gingiva-derived and bone marrow stem cells using concave microwells: J.Y. Tae, et al.; Exp. Ther. Med.
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Osterix regulates corticalization for longitudinal bone growth via integrin β3 expression: Y.J. Moon, et al.; Exp. Mol. Med.
50, 80 (2018),
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Priming stem cells with protein kinase C activator enhances early stem cell-chondrocyte interaction by increasing adhesion molecules: D. Chae, et al.; Biol. Res.
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Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes: D. Shin, et al.; BMC Complement. Altern. Med.
18, 196 (2018),
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Full Text
Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis: J.H. Kim, et al.; Parasite Immunol.
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Protective properties of geniposide against UV-B-induced photooxidative stress in human dermal fibroblasts: D. Shin, et al.; Pharm. Biol.
56, 176 (2018),
Abstract;
Full Text
Resveratrol induces mitochondrial apoptosis and inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma cells: S.E. Kim, et al.; Nutr. Cancer
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Resveratrol-mediated apoptosis in renal cell carcinoma via the p53/AMP‑activated protein kinase/mammalian target of rapamycin autophagy signaling pathway: Q. Liu, et al.; Mol. Med. Rep.
17, 502 (2018),
Abstract;
The role of AP-1 in self-sufficient proliferation and migration of cancer cells and its potential impact on an autocrine/paracrine loop: S.A.E. Ibrahim, et al.; Oncotarget
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Abstract;
Full Text
Tristetraprolin activation by resveratrol inhibits the proliferation and metastasis of colorectal cancer cells: S.R. Lee, et al.; Int. J. Oncol.
53, 1269 (2018),
Abstract;
Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One
12, e0183028 (2017),
Application(s): Western Blot,
Abstract;
Full Text
cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice: X. Gui, et al.; Mol. Med. Rep.
15, 221 (2017),
Abstract;
Full Text
Fluid shear stress regulates vascular remodeling via VEGFR-3 activation, although independently of its ligand, VEGF-C, in the uterus during pregnancy: Y.G. Park, et al.; Int. J. Mol. Med.
40, 1210 (2017),
Abstract;
Full Text
Ginsenoside Rc protects against UVB‑induced photooxidative damage in epidermal keratinocytes: Y. Oh, et al.; Mol. Med. Rep.
16, 2907 (2017),
Abstract;
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover: J.C. Capaz, et al.; Front. Physiol.
8, 344 (2017),
Abstract;
Full Text
Inhibitory effect of brassinin on TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells: B.H. Han, et al.; Mol. Med. Rep.
16, 6890 (2017),
Abstract;
Korean Scutellaria baicalensis Georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells: V. Ventatarame Gowda Saralamma, et al.; Oncol. Lett.
14, 607 (2017),
Abstract;
Full Text
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array: S. Yumnam, et al.; Mol. Med. Rep.
16, 5386 (2017),
Abstract;
Full Text
Quantification of heat shock protein 70 and acetylcholinesterase over a time course suggests environmental adaptation in a foundational molluscan species: A. Ravaschiere, et al.; Ecotoxicol. Environ. Saf.
142, 222 (2017),
Abstract;
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones
22, 225 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Wiryeongtang regulates hypertonicity-induced expression of aquaporin-2 water channels in mIMCD-3 cells: M.G. Kim, et al.; Mol. Med. Rep.
15, 2665 (2017),
Abstract;
Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line: Y. Oh, et al.; J. Photochem. Photobiol. B
163, 170 (2016),
Application(s): Western blot, detect proMMP-2 and -9 proteins in cellular lysate,
Abstract;
Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane: M. Amarasiri, et al.; Water Res.
95, 383 (2016),
Abstract;
Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats: M. Ram, et al.; Int. Immunopharmacol.
30, 137 (2016),
Application(s): Western blot,
Abstract;
Bottom-up proteomics suggests an association between differential expression of mitochondrial proteins and chronic fatigue syndrome: F. Ciregia, et al.; Transl. Psychiatry
6, e904 (2016),
Application(s): Western blot,
Abstract;
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1: H.S. Seol, et al.; Cancer Lett.
372, 24 (2016),
Abstract;
Full Text
Contribution of ginsenoside Re to cellular redox homeostasis via upregulating glutathione and superoxide dismutase in HaCaT keratinocytes under normal conditions: H.W. Lim, et al.; Pharmazie
71, 413 (2016),
Abstract;
Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts: B. Beomyeol, et al.; Korean J. Physiol. Pharmacol.
20, 269 (2016),
Application(s): Western blot,
Abstract;
Full Text
Hypoxia‑mediated activation of autophagic flux inhibits apoptosis of keratinocytes via blocking tumor necrosis factor‑related apoptosis‑inducing ligand: S.W. Kim, et al.; Mol. Med. Rep.
13, 805 (2016),
Abstract;
NEB-related core-rod myopathy with distinct clinical and pathological features: Y.E. Park, et al.; Muscle Nerve
53, 479 (2016),
Abstract;
Pass the salt: physiological consequences of ecologically relevant hyposmotic exposure in juvenile gummy sharks (Mustelus antarcticus) and school sharks (Galeorhinus galeus): A.J. Morash, et al.; Conserv. Physiol.
4, cow036 (2016),
Abstract;
Full Text
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep.
6, 29389 (2016),
Application(s): Antibody - immunofluorescence assay,
Abstract;
Full Text
Photoprotective properties of 20(S)-protopanaxatriol, an aglycone of ginseng saponins: Protection from ultraviolet-B radiation-induced oxidative stress in human epidermal keratinocytes: S.J. Oh, et al.; Mol. Med. Rep.
14, 2839 (2016),
Abstract;
Plasma glutathione reductase activity and prognosis of septic shock: J.S. Kim, et al.; J. Surg. Res.
200, 298 (2016),
Application(s): Western Blot,
Abstract;
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated With Trichomonas vaginalis via Crosstalk With Mast Cell: J.H. Kim, et al.; Prostate
76, 1431 (2016),
Abstract;
Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein: Y. Sim, et al.; Neuroscience
340, 166 (2016),
Application(s): Western Blot,
Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed.
11, 2407 (2016),
Application(s): Western blot,
Abstract;
Full Text
Stereospecificity of ginsenoside Rg2 epimers in the protective response against UV-B radiation-induced oxidative stress in human epidermal keratinocytes: H.J. Kang, et al.; J. Photochem. Photobiol. B.
165, 232 (2016),
Application(s): Western blot, cell lysate,
Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci.
161, 37 (2016),
Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation,
Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res.
48, 813 (2015),
Application(s): Western Blot,
Abstract;
Full Text
An Aβ42 uptake and degradation via Rg3 requires an activation of caveolin, clathrin and Aβ-degrading enzymes in microglia: S. K. Jang, et al.; Eur. J. Pharmacol.
758, 1 (2015),
Application(s): Western Blot,
Abstract;
Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact.
237, 104 (2015),
Application(s): Western Blot,
Abstract;
Chronic nicotine treatment enhances vascular smooth muscle relaxation in rats: T. Y. Xu, et al.; Acta. Pharmacol. Sin.
36, 429 (2015),
Application(s): Chemiluminescence Western Blot,
Abstract;
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl).
128, 1465 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta
1853, 996 (2015),
Application(s): Western Blotting,
Abstract;
Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice: S.J. Woo, et al.; J. Nutr. Biochem.
26, 713 (2015),
Application(s): Western Blotting,
Abstract;
Host responses associated with chronic staphylococcal mastitis in rabbits: I. Guerrero, et al.; Vet. J.
204, 338 (2015),
Application(s): Immunohistochemistry,
Abstract;
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β2-adrenergic receptor activation: J.W. Eng, et al.; Nat. Commun.
6, 6426 (2015),
Application(s): Western Blotting,
Abstract;
Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest: S.J. Park, et al.; Am. J. Cancer Res.
5, 2127 (2015),
Abstract;
Full Text
Mori Fructus improves cognitive and neuronal dysfunction induced by beta-amyloid toxicity through the GSK-3β pathway in vitro and in vivo: H.G. Kim, et al.; J. Ethnopharmacol.
171, 196 (2015),
Application(s): Immunocytochemistry and immunohistochemistry,
Abstract;
SERCA-2a is Involved in the Right Ventricular Function Following Myocardial Infarction in Rats: A.A. Fernandes, et al.; Life Sci.
124, 24 (2015),
Application(s): Western Blotting,
Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One
10, e0115334 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Functional variants of human APE1 rescue the DNA repair defects of the yeast AP endonuclease/3'-diesterase-deficient strain: Z. Wang, et al.; DNA Repair (Amst)
22C, 53 (2014),
Abstract;
Mechanisms responsible for the acceleration of pulmonary V'O2 on-kinetics in humans after prolonged endurance training: J.A. Zoladz, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
307, R1101 (2014),
Abstract;
Skin anti-photoaging properties of ginsenoside Rh2 epimers in UV-B-irradiated human keratinocyte cells: S.J. Oh, et al.; J. Biosci.
39, 673 (2014),
Application(s): WB using human cell supernatant,
Abstract;
Protein kinase C activation stimulates mesenchymal stem cell adhesion through activation of focal adhesion kinase: B.W. Song, et al.; Cell Transplant.
22, 797 (2013),
Application(s): WB using rat cell lysates,
Abstract;
Sublethal effects of silver nanoparticles and dissolved silver in freshwater mussels: F. Gagné, et al.; J. Toxicol. Environ. Health A
76, 479 (2013),
Abstract;
Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis.
52, 972 (2008),
Application(s): IHC using human tissue,
Abstract;
Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells: T. Acosta, et al. ; J. Endocrinol.
199, 425 (2008),
Application(s): WB using rabbit cell lysates,
Abstract;
NAD(P)H oxidase modulates angiogenesis and the development of portosystemic collaterals and splanchnic hyperaemia in portal hypertensive rats: B. Angermayr, et al.; Gut
56, 560 (2007),
Application(s): WB using rat tissue,
Abstract;
Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain: H.R. Wong, et al. ; Biochem. Biophys. Res. Commun.
301, 54 (2003),
Application(s): WB using mouse samples,
Abstract;
Involvement of heme oxygenase-1 (HO-1) in the adaptive protection of human lymphocytes after hyperbaric oxygen (HBO) treatment: G. Speit, et al. ; Carcinogenesis
22, 1979 (2001),
Application(s): WB using human samples,
Abstract;
p38 MAPK mediates renal tubular cell TNF-alpha production and TNF-alpha-dependent apoptosis during simulated ischemia: M.A. Kaefer, et al. ; Am. J. Physiol. Cell Physiol.
281, C563 (2001),
Application(s): WB using porcine samples,
Abstract;
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