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Goat anti-rabbit IgG, polyclonal antibody (HRP conjugate)

ADI-SAB-300-J 1 ml 118.00 USD
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Product Specification

Immunogen:Purified rabbit IgG (whole molecule).
Species reactivity:Rabbit
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500, Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Immunoaffinity purified.
Quality Control:The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.
Formulation:Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA (IgG-free, protease-free) and 50% glycerol.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.

Product Literature References

Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One 12, e0183028 (2017), Application(s): Western Blot, Abstract; Full Text
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones 22, 225 (2017), Application(s): Western Blot, Abstract; Full Text
Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line: Y. Oh, et al.; J. Photochem. Photobiol. B 163, 170 (2016), Application(s): Western blot, detect proMMP-2 and -9 proteins in cellular lysate, Abstract;
Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats: M. Ram, et al.; Int. Immunopharmacol. 30, 137 (2016), Application(s): Western blot, Abstract;
Bottom-up proteomics suggests an association between differential expression of mitochondrial proteins and chronic fatigue syndrome: F. Ciregia, et al.; Transl. Psychiatry 6, e904 (2016), Application(s): Western blot, Abstract;
Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts: B. Beomyeol, et al.; Korean J. Physiol. Pharmacol. 20, 269 (2016), Application(s): Western blot, Abstract; Full Text
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep. 6, 29389 (2016), Application(s): Antibody - immunofluorescence assay, Abstract; Full Text
Plasma glutathione reductase activity and prognosis of septic shock: J.S. Kim, et al.; J. Surg. Res. 200, 298 (2016), Application(s): Western Blot, Abstract;
Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein: Y. Sim, et al.; Neuroscience 340, 166 (2016), Application(s): Western Blot, Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed. 11, 2407 (2016), Application(s): Western blot, Abstract; Full Text
Stereospecificity of ginsenoside Rg2 epimers in the protective response against UV-B radiation-induced oxidative stress in human epidermal keratinocytes: H.J. Kang, et al.; J. Photochem. Photobiol. B. 165, 232 (2016), Application(s): Western blot, cell lysate, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation, Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res. 48, 813 (2015), Application(s): Western Blot, Abstract; Full Text
An Aβ42 uptake and degradation via Rg3 requires an activation of caveolin, clathrin and Aβ-degrading enzymes in microglia: S. K. Jang, et al.; Eur. J. Pharmacol. 758, 1 (2015), Application(s): Western Blot, Abstract;
Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact. 237, 104 (2015), Application(s): Western Blot, Abstract;
Chronic nicotine treatment enhances vascular smooth muscle relaxation in rats: T. Y. Xu, et al.; Acta. Pharmacol. Sin. 36, 429 (2015), Application(s): Chemiluminescence Western Blot, Abstract;
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl). 128, 1465 (2015), Application(s): Western Blot, Abstract; Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice: S.J. Woo, et al.; J. Nutr. Biochem. 26, 713 (2015), Application(s): Western Blotting, Abstract;
Host responses associated with chronic staphylococcal mastitis in rabbits: I. Guerrero, et al.; Vet. J. 204, 338 (2015), Application(s): Immunohistochemistry, Abstract;
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β2-adrenergic receptor activation: J.W. Eng, et al.; Nat. Commun. 6, 6426 (2015), Application(s): Western Blotting, Abstract;
Mori Fructus improves cognitive and neuronal dysfunction induced by beta-amyloid toxicity through the GSK-3β pathway in vitro and in vivo: H.G. Kim, et al.; J. Ethnopharmacol. 171, 196 (2015), Application(s): Immunocytochemistry and immunohistochemistry, Abstract;
SERCA-2a is Involved in the Right Ventricular Function Following Myocardial Infarction in Rats: A.A. Fernandes, et al.; Life Sci. 124, 24 (2015), Application(s): Western Blotting, Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
Functional variants of human APE1 rescue the DNA repair defects of the yeast AP endonuclease/3'-diesterase-deficient strain: Z. Wang, et al.; DNA Repair (Amst) 22C, 53 (2014), Abstract;
Mechanisms responsible for the acceleration of pulmonary V'O2 on-kinetics in humans after prolonged endurance training: J.A. Zoladz, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 307, R1101 (2014), Abstract;
Skin anti-photoaging properties of ginsenoside Rh2 epimers in UV-B-irradiated human keratinocyte cells: S.J. Oh, et al.; J. Biosci. 39, 673 (2014), Application(s): WB using human cell supernatant, Abstract;
Protein kinase C activation stimulates mesenchymal stem cell adhesion through activation of focal adhesion kinase: B.W. Song, et al.; Cell Transplant. 22, 797 (2013), Application(s): WB using rat cell lysates, Abstract;
Sublethal effects of silver nanoparticles and dissolved silver in freshwater mussels: F. Gagné, et al.; J. Toxicol. Environ. Health A 76, 479 (2013), Abstract;
Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis. 52, 972 (2008), Application(s): IHC using human tissue, Abstract;
Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells: T. Acosta, et al. ; J. Endocrinol. 199, 425 (2008), Application(s): WB using rabbit cell lysates, Abstract;
NAD(P)H oxidase modulates angiogenesis and the development of portosystemic collaterals and splanchnic hyperaemia in portal hypertensive rats: B. Angermayr, et al.; Gut 56, 560 (2007), Application(s): WB using rat tissue, Abstract;
Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain: H.R. Wong, et al. ; Biochem. Biophys. Res. Commun. 301, 54 (2003), Application(s): WB using mouse samples, Abstract;
Involvement of heme oxygenase-1 (HO-1) in the adaptive protection of human lymphocytes after hyperbaric oxygen (HBO) treatment: G. Speit, et al. ; Carcinogenesis 22, 1979 (2001), Application(s): WB using human samples, Abstract;
p38 MAPK mediates renal tubular cell TNF-alpha production and TNF-alpha-dependent apoptosis during simulated ischemia: M.A. Kaefer, et al. ; Am. J. Physiol. Cell Physiol. 281, C563 (2001), Application(s): WB using porcine samples, Abstract;

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