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Goat anti-rabbit IgG, polyclonal antibody (HRP conjugate)

ADI-SAB-300-J 1 ml 124.00 USD
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Product Specification

Immunogen:Purified rabbit IgG (whole molecule).
Species reactivity:Rabbit
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500, Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Immunoaffinity purified.
Quality Control:The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.
Formulation:Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA (IgG-free, protease-free) and 50% glycerol.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.

Product Literature References

Essential oil from Korean Chamaecyparis obtusa leaf ameliorates respiratory activity in Sprague‑Dawley rats and exhibits protection from NF-κB-induced inflammation in WI38 fibroblast cells: S. Raha, et al.; Int. J. Mol. Med. 43, 393 (2019), Abstract; Full Text
Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate: E. A. Park, et al.; Korean J. Parasitol. 57, 201 (2019), Abstract; Full Text
The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome: M.C. Trotta, et al.; PLoS One 14, e0211005 (2019), Abstract; Full Text
Transgenic overexpression of human LY6K in mice suppresses mature T cell development in the thymus: D. Son, et al.; Oncol. Lett. 17, 379 (2019), Abstract; Full Text
An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAK/NF-κB Signaling Pathways: J.Y. Lee, et al.; Front. Aging Neurosci. 10, 269 (2018), Abstract; Full Text
Anticancer and radiosensitizing effects of the cyclin-dependent kinase inhibitors, AT7519 and SNS‑032, on cervical cancer: M.S. Kang, et al.; Int. J. Oncol. 53, 703 (2018), Abstract;
Combined therapy involving electroacupuncture and treadmillexercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp Neurol. 300, 222 (2018), Application(s): Western Blot, Abstract;
Defensive Properties of Ginsenoside Re against UV-B-Induced Oxidative Stress through Up-Regulating Glutathione and Superoxide Dismutase in HaCaT Keratinocytes: D. Shin, et al.; Iran. J. Pharm. Res. 17, 249 (2018), Abstract; Full Text
Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer: C.H. Choi, et al.; Sci. Rep. 8, 12161 (2018), Abstract; Full Text
Inhibition of aldose-reductase-2 by a benzofuroxane derivative bf-5m increases the expression of kcne1, kcnq1 in high glucose cultured H9c2 cardiac cells and sudden cardiac death: M.C. Trotta, et al.; Oncotarget 9, 17257 (2018), Abstract; Full Text
Osteogenic potential of cell spheroids composed of varying ratios of gingiva-derived and bone marrow stem cells using concave microwells: J.Y. Tae, et al.; Exp. Ther. Med. 16, 2287 (2018), Abstract; Full Text
Osterix regulates corticalization for longitudinal bone growth via integrin β3 expression: Y.J. Moon, et al.; Exp. Mol. Med. 50, 80 (2018), Abstract; Full Text
Priming stem cells with protein kinase C activator enhances early stem cell-chondrocyte interaction by increasing adhesion molecules: D. Chae, et al.; Biol. Res. 51, 41 (2018), Abstract; Full Text
Probiotic fermentation augments the skin anti-photoaging properties of Agastache rugosa through up-regulating antioxidant components in UV-B-irradiated HaCaT keratinocytes: D. Shin, et al.; BMC Complement. Altern. Med. 18, 196 (2018), Abstract; Full Text
Proliferation of prostate epithelia induced by IL-6 from stroma reacted with Trichomonas vaginalis: J.H. Kim, et al.; Parasite Immunol. 40, e12531 (2018), Abstract;
Protective properties of geniposide against UV-B-induced photooxidative stress in human dermal fibroblasts: D. Shin, et al.; Pharm. Biol. 56, 176 (2018), Abstract; Full Text
Resveratrol induces mitochondrial apoptosis and inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma cells: S.E. Kim, et al.; Nutr. Cancer 70, 125 (2018), Abstract;
Resveratrol-mediated apoptosis in renal cell carcinoma via the p53/AMP‑activated protein kinase/mammalian target of rapamycin autophagy signaling pathway: Q. Liu, et al.; Mol. Med. Rep. 17, 502 (2018), Abstract;
The role of AP-1 in self-sufficient proliferation and migration of cancer cells and its potential impact on an autocrine/paracrine loop: S.A.E. Ibrahim, et al.; Oncotarget 9, 34259 (2018), Abstract; Full Text
Tristetraprolin activation by resveratrol inhibits the proliferation and metastasis of colorectal cancer cells: S.R. Lee, et al.; Int. J. Oncol. 53, 1269 (2018), Abstract;
Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One 12, e0183028 (2017), Application(s): Western Blot, Abstract; Full Text
cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice: X. Gui, et al.; Mol. Med. Rep. 15, 221 (2017), Abstract; Full Text
Fluid shear stress regulates vascular remodeling via VEGFR-3 activation, although independently of its ligand, VEGF-C, in the uterus during pregnancy: Y.G. Park, et al.; Int. J. Mol. Med. 40, 1210 (2017), Abstract; Full Text
Ginsenoside Rc protects against UVB‑induced photooxidative damage in epidermal keratinocytes: Y. Oh, et al.; Mol. Med. Rep. 16, 2907 (2017), Abstract;
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover: J.C. Capaz, et al.; Front. Physiol. 8, 344 (2017), Abstract; Full Text
Inhibitory effect of brassinin on TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells: B.H. Han, et al.; Mol. Med. Rep. 16, 6890 (2017), Abstract;
Korean Scutellaria baicalensis Georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells: V. Ventatarame Gowda Saralamma, et al.; Oncol. Lett. 14, 607 (2017), Abstract; Full Text
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array: S. Yumnam, et al.; Mol. Med. Rep. 16, 5386 (2017), Abstract; Full Text
Quantification of heat shock protein 70 and acetylcholinesterase over a time course suggests environmental adaptation in a foundational molluscan species: A. Ravaschiere, et al.; Ecotoxicol. Environ. Saf. 142, 222 (2017), Abstract;
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones 22, 225 (2017), Application(s): Western Blot, Abstract; Full Text
Wiryeongtang regulates hypertonicity-induced expression of aquaporin-2 water channels in mIMCD-3 cells: M.G. Kim, et al.; Mol. Med. Rep. 15, 2665 (2017), Abstract;
Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line: Y. Oh, et al.; J. Photochem. Photobiol. B 163, 170 (2016), Application(s): Western blot, detect proMMP-2 and -9 proteins in cellular lysate, Abstract;
Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane: M. Amarasiri, et al.; Water Res. 95, 383 (2016), Abstract;
Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats: M. Ram, et al.; Int. Immunopharmacol. 30, 137 (2016), Application(s): Western blot, Abstract;
Bottom-up proteomics suggests an association between differential expression of mitochondrial proteins and chronic fatigue syndrome: F. Ciregia, et al.; Transl. Psychiatry 6, e904 (2016), Application(s): Western blot, Abstract;
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1: H.S. Seol, et al.; Cancer Lett. 372, 24 (2016), Abstract; Full Text
Contribution of ginsenoside Re to cellular redox homeostasis via upregulating glutathione and superoxide dismutase in HaCaT keratinocytes under normal conditions: H.W. Lim, et al.; Pharmazie 71, 413 (2016), Abstract;
Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts: B. Beomyeol, et al.; Korean J. Physiol. Pharmacol. 20, 269 (2016), Application(s): Western blot, Abstract; Full Text
Hypoxia‑mediated activation of autophagic flux inhibits apoptosis of keratinocytes via blocking tumor necrosis factor‑related apoptosis‑inducing ligand: S.W. Kim, et al.; Mol. Med. Rep. 13, 805 (2016), Abstract;
NEB-related core-rod myopathy with distinct clinical and pathological features: Y.E. Park, et al.; Muscle Nerve 53, 479 (2016), Abstract;
Pass the salt: physiological consequences of ecologically relevant hyposmotic exposure in juvenile gummy sharks (Mustelus antarcticus) and school sharks (Galeorhinus galeus): A.J. Morash, et al.; Conserv. Physiol. 4, cow036 (2016), Abstract; Full Text
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep. 6, 29389 (2016), Application(s): Antibody - immunofluorescence assay, Abstract; Full Text
Photoprotective properties of 20(S)-protopanaxatriol, an aglycone of ginseng saponins: Protection from ultraviolet-B radiation-induced oxidative stress in human epidermal keratinocytes: S.J. Oh, et al.; Mol. Med. Rep. 14, 2839 (2016), Abstract;
Plasma glutathione reductase activity and prognosis of septic shock: J.S. Kim, et al.; J. Surg. Res. 200, 298 (2016), Application(s): Western Blot, Abstract;
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated With Trichomonas vaginalis via Crosstalk With Mast Cell: J.H. Kim, et al.; Prostate 76, 1431 (2016), Abstract;
Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein: Y. Sim, et al.; Neuroscience 340, 166 (2016), Application(s): Western Blot, Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed. 11, 2407 (2016), Application(s): Western blot, Abstract; Full Text
Stereospecificity of ginsenoside Rg2 epimers in the protective response against UV-B radiation-induced oxidative stress in human epidermal keratinocytes: H.J. Kang, et al.; J. Photochem. Photobiol. B. 165, 232 (2016), Application(s): Western blot, cell lysate, Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation, Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res. 48, 813 (2015), Application(s): Western Blot, Abstract; Full Text
An Aβ42 uptake and degradation via Rg3 requires an activation of caveolin, clathrin and Aβ-degrading enzymes in microglia: S. K. Jang, et al.; Eur. J. Pharmacol. 758, 1 (2015), Application(s): Western Blot, Abstract;
Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact. 237, 104 (2015), Application(s): Western Blot, Abstract;
Chronic nicotine treatment enhances vascular smooth muscle relaxation in rats: T. Y. Xu, et al.; Acta. Pharmacol. Sin. 36, 429 (2015), Application(s): Chemiluminescence Western Blot, Abstract;
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl). 128, 1465 (2015), Application(s): Western Blot, Abstract; Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta 1853, 996 (2015), Application(s): Western Blotting, Abstract;
Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice: S.J. Woo, et al.; J. Nutr. Biochem. 26, 713 (2015), Application(s): Western Blotting, Abstract;
Host responses associated with chronic staphylococcal mastitis in rabbits: I. Guerrero, et al.; Vet. J. 204, 338 (2015), Application(s): Immunohistochemistry, Abstract;
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β2-adrenergic receptor activation: J.W. Eng, et al.; Nat. Commun. 6, 6426 (2015), Application(s): Western Blotting, Abstract;
Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest: S.J. Park, et al.; Am. J. Cancer Res. 5, 2127 (2015), Abstract; Full Text
Mori Fructus improves cognitive and neuronal dysfunction induced by beta-amyloid toxicity through the GSK-3β pathway in vitro and in vivo: H.G. Kim, et al.; J. Ethnopharmacol. 171, 196 (2015), Application(s): Immunocytochemistry and immunohistochemistry, Abstract;
SERCA-2a is Involved in the Right Ventricular Function Following Myocardial Infarction in Rats: A.A. Fernandes, et al.; Life Sci. 124, 24 (2015), Application(s): Western Blotting, Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
Functional variants of human APE1 rescue the DNA repair defects of the yeast AP endonuclease/3'-diesterase-deficient strain: Z. Wang, et al.; DNA Repair (Amst) 22C, 53 (2014), Abstract;
Mechanisms responsible for the acceleration of pulmonary V'O2 on-kinetics in humans after prolonged endurance training: J.A. Zoladz, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 307, R1101 (2014), Abstract;
Skin anti-photoaging properties of ginsenoside Rh2 epimers in UV-B-irradiated human keratinocyte cells: S.J. Oh, et al.; J. Biosci. 39, 673 (2014), Application(s): WB using human cell supernatant, Abstract;
Protein kinase C activation stimulates mesenchymal stem cell adhesion through activation of focal adhesion kinase: B.W. Song, et al.; Cell Transplant. 22, 797 (2013), Application(s): WB using rat cell lysates, Abstract;
Sublethal effects of silver nanoparticles and dissolved silver in freshwater mussels: F. Gagné, et al.; J. Toxicol. Environ. Health A 76, 479 (2013), Abstract;
Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis. 52, 972 (2008), Application(s): IHC using human tissue, Abstract;
Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells: T. Acosta, et al. ; J. Endocrinol. 199, 425 (2008), Application(s): WB using rabbit cell lysates, Abstract;
NAD(P)H oxidase modulates angiogenesis and the development of portosystemic collaterals and splanchnic hyperaemia in portal hypertensive rats: B. Angermayr, et al.; Gut 56, 560 (2007), Application(s): WB using rat tissue, Abstract;
Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain: H.R. Wong, et al. ; Biochem. Biophys. Res. Commun. 301, 54 (2003), Application(s): WB using mouse samples, Abstract;
Involvement of heme oxygenase-1 (HO-1) in the adaptive protection of human lymphocytes after hyperbaric oxygen (HBO) treatment: G. Speit, et al. ; Carcinogenesis 22, 1979 (2001), Application(s): WB using human samples, Abstract;
p38 MAPK mediates renal tubular cell TNF-alpha production and TNF-alpha-dependent apoptosis during simulated ischemia: M.A. Kaefer, et al. ; Am. J. Physiol. Cell Physiol. 281, C563 (2001), Application(s): WB using porcine samples, Abstract;

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