Product Specification
| Host: | Goat |
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| Immunogen: | Purified rabbit IgG (whole molecule). |
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| Species reactivity: | Rabbit
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| Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. |
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| Applications: | ELISA, IHC, WB
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| Recommended Dilutions/Conditions: | ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500, Western Blot (1:5,000-100,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Immunoaffinity purified. |
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| Quality Control: | The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species. |
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| Formulation: | Liquid. In PBS, pH 7.6, containing 7.5mg/mL BSA (IgG-free, protease-free) and 50% glycerol. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG. |
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Product Literature References
Attenuating properties of Agastache rugosa leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismutase in a human keratinocyte cell line: Y. Oh, et al.; J. Photochem. Photobiol. B
163, 170 (2016),
Application(s): Western blot, detect proMMP-2 and -9 proteins in cellular lysate,
Abstract;
Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats: M. Ram, et al.; Int. Immunopharmacol.
30, 137 (2016),
Application(s): Western blot,
Abstract;
Bottom-up proteomics suggests an association between differential expression of mitochondrial proteins and chronic fatigue syndrome: F. Ciregia, et al.; Transl. Psychiatry
6, e904 (2016),
Application(s): Western blot,
Abstract;
Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts: B. Beomyeol, et al.; Korean J. Physiol. Pharmacol.
20, 269 (2016),
Application(s): Western blot,
Abstract;
Full Text
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep.
6, 29389 (2016),
Application(s): Antibody - immunofluorescence assay,
Abstract;
Full Text
Plasma glutathione reductase activity and prognosis of septic shock: J.S. Kim, et al.; J. Surg. Res.
200, 298 (2016),
Application(s): Western Blot,
Abstract;
Protective effects of a herbal extract combination of Bupleurum falcatum, Paeonia suffruticosa, and Angelica dahurica against MPTP-induced neurotoxicity via regulation of nuclear receptor-related 1 protein: Y. Sim, et al.; Neuroscience
340, 166 (2016),
Application(s): Western Blot,
Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed.
11, 2407 (2016),
Application(s): Western blot,
Abstract;
Full Text
Stereospecificity of ginsenoside Rg2 epimers in the protective response against UV-B radiation-induced oxidative stress in human epidermal keratinocytes: H.J. Kang, et al.; J. Photochem. Photobiol. B.
165, 232 (2016),
Application(s): Western blot, cell lysate,
Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci.
161, 37 (2016),
Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation,
Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res.
48, 813 (2015),
Application(s): Western Blot,
Abstract;
Full Text
An Aβ42 uptake and degradation via Rg3 requires an activation of caveolin, clathrin and Aβ-degrading enzymes in microglia: S. K. Jang, et al.; Eur. J. Pharmacol.
758, 1 (2015),
Application(s): Western Blot,
Abstract;
Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact.
237, 104 (2015),
Application(s): Western Blot,
Abstract;
Chronic nicotine treatment enhances vascular smooth muscle relaxation in rats: T. Y. Xu, et al.; Acta. Pharmacol. Sin.
36, 429 (2015),
Application(s): Chemiluminescence Western Blot,
Abstract;
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl).
128, 1465 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta
1853, 996 (2015),
Application(s): Western Blotting,
Abstract;
Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice: S.J. Woo, et al.; J. Nutr. Biochem.
26, 713 (2015),
Application(s): Western Blotting,
Abstract;
Host responses associated with chronic staphylococcal mastitis in rabbits: I. Guerrero, et al.; Vet. J.
204, 338 (2015),
Application(s): Immunohistochemistry,
Abstract;
Housing temperature-induced stress drives therapeutic resistance in murine tumour models through β2-adrenergic receptor activation: J.W. Eng, et al.; Nat. Commun.
6, 6426 (2015),
Application(s): Western Blotting,
Abstract;
Mori Fructus improves cognitive and neuronal dysfunction induced by beta-amyloid toxicity through the GSK-3β pathway in vitro and in vivo: H.G. Kim, et al.; J. Ethnopharmacol.
171, 196 (2015),
Application(s): Immunocytochemistry and immunohistochemistry,
Abstract;
SERCA-2a is Involved in the Right Ventricular Function Following Myocardial Infarction in Rats: A.A. Fernandes, et al.; Life Sci.
124, 24 (2015),
Application(s): Western Blotting,
Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One
10, e0115334 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Functional variants of human APE1 rescue the DNA repair defects of the yeast AP endonuclease/3'-diesterase-deficient strain: Z. Wang, et al.; DNA Repair (Amst)
22C, 53 (2014),
Abstract;
Mechanisms responsible for the acceleration of pulmonary V'O2 on-kinetics in humans after prolonged endurance training: J.A. Zoladz, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
307, R1101 (2014),
Abstract;
Skin anti-photoaging properties of ginsenoside Rh2 epimers in UV-B-irradiated human keratinocyte cells: S.J. Oh, et al.; J. Biosci.
39, 673 (2014),
Application(s): WB using human cell supernatant,
Abstract;
Protein kinase C activation stimulates mesenchymal stem cell adhesion through activation of focal adhesion kinase: B.W. Song, et al.; Cell Transplant.
22, 797 (2013),
Application(s): WB using rat cell lysates,
Abstract;
Sublethal effects of silver nanoparticles and dissolved silver in freshwater mussels: F. Gagné, et al.; J. Toxicol. Environ. Health A
76, 479 (2013),
Abstract;
Induction of Heme Oxygenase-1 and Ferritin in the Kidney in Warm Antibody Hemolytic Anemia: F. Fervenza, et al. ; Am. J. Kidney Dis.
52, 972 (2008),
Application(s): IHC using human tissue,
Abstract;
Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells: T. Acosta, et al. ; J. Endocrinol.
199, 425 (2008),
Application(s): WB using rabbit cell lysates,
Abstract;
NAD(P)H oxidase modulates angiogenesis and the development of portosystemic collaterals and splanchnic hyperaemia in portal hypertensive rats: B. Angermayr, et al.; Gut
56, 560 (2007),
Application(s): WB using rat tissue,
Abstract;
Intracellular delivery of HSP70 using HIV-1 Tat protein transduction domain: H.R. Wong, et al. ; Biochem. Biophys. Res. Commun.
301, 54 (2003),
Application(s): WB using mouse samples,
Abstract;
Involvement of heme oxygenase-1 (HO-1) in the adaptive protection of human lymphocytes after hyperbaric oxygen (HBO) treatment: G. Speit, et al. ; Carcinogenesis
22, 1979 (2001),
Application(s): WB using human samples,
Abstract;
p38 MAPK mediates renal tubular cell TNF-alpha production and TNF-alpha-dependent apoptosis during simulated ischemia: M.A. Kaefer, et al. ; Am. J. Physiol. Cell Physiol.
281, C563 (2001),
Application(s): WB using porcine samples,
Abstract;
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