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Goat anti-rat IgG, polyclonal antibody (HRP conjugate)

 
ADI-SAB-220-0500 0.5 ml 215.00 USD
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Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, mouse, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

Product Specification

Host:Goat
 
Immunogen:Purified rat IgG (whole molecule).
 
Species reactivity:Rat
 
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
 
Applications:ELISA, ICC, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (1:5,000-1:100,000)
Immunocytochemistry (1:500-1:5,000)
Immunohistochemistry(1:500-1:5,000)
Western Blot (1:10,000-1:200,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Quality Control:The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, mouse, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
 
Formulation:Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol.
 
Use/Stability:Warning that sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains.  Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain.
 

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