Product Details
| Host: | Goat |
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| Immunogen: | Purified mouse IgM. |
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| Species reactivity: | Mouse
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| Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. |
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| Crossreactivity: | May cross-react with IgM from other species. |
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| Applications: | ELISA, IHC, WB
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| Recommended Dilutions/Conditions: | ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500)
Western Blot (1:2500-50,000 colorimetric, 1:5,000-100,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Immunoaffinity purified. |
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| Quality Control: | The goat antiserum was immunoaffinity purified to isolate anti-mouse IgM μ-chain specific antibodies which were then adsorbed with human IgG and conjugated to horseradish peroxidase (HRP). |
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| Formulation: | Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/mL BSA, and 50% glycerol. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection. |
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| Regulatory Status: | RUO - Research Use Only |
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Application(s): Immunoblot assay,
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Application(s): Western Blot,
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p38 MAPK contributes to angiotensin II-induced COX-2 expression in aortic fibroblasts from normotensive and hypertensive rats: M. Salaices, et al. ; J. Hypertens.
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Application(s): EIA using mouse samples,
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Application(s): WB using mouse samples,
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183, 69 (2007),
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