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Goat anti-mouse IgM, polyclonal antibody (HRP conjugate)

 
ADI-SAB-110-J 1 ml 118.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Host:Goat
 
Immunogen:Purified mouse IgM.
 
Species reactivity:Mouse
 
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of mouse IgM but not with mouse IgG or the light chains of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
 
Crossreactivity:May cross-react with IgM from other species.
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (1:2,500-50,000)
Immunohistochemistry (1:250-2500)
Western Blot (1:2500-50,000 colorimetric, 1:5,000-100,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Quality Control:The goat antiserum was immunoaffinity purified to isolate anti-mouse IgM μ-chain specific antibodies which were then adsorbed with human IgG and conjugated to horseradish peroxidase (HRP).
 
Formulation:Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/mL BSA, and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection.
 

Product Literature References

A Single Resistance Exercise Session Improves Aortic Endothelial Function in Hypertensive Rats: T. de Oliveria Faria, et al.; Arq. Bras. Cardiol. 108, 228 (2017), Abstract; Full Text
Bacterial Heat Shock Protein GroEL (Hsp64) Exerts Immunoregulatory Effects on T Cells by Utilizing Apoptosis: A.M. Nalbant, et al.; PLoS One 11, e0164380 (2016), Application(s): Preparation of the GroEL antigen, Abstract; Full Text
Deflection induced cellular focal adhesion and anisotropic growth on vertically aligned silicon nanowires with differing elasticity: Y.S. Park, et al.; NPG Asia Materials 8, e249 (2016), Application(s): Immunoblot assay, Full Text
Low-salt diet increases NO bioavailability and COX-2 vasoconstrictor prostanoid production in spontaneously hypertensive rats: T.C. Travaglia, et al.; Life Sci. 145, 66 (2016), Application(s): Western blot, Abstract;
An Aβ42 uptake and degradation via Rg3 requires an activation of caveolin, clathrin and Aβ-degrading enzymes in microglia: S. K. Jang, et al.; Eur. J. Pharmacol. 758, 1 (2015), Application(s): Western Blot, Abstract;
Gedunin Binds to MD-2 and Impairs LPS-Induced TLR4 Signaling in Macrophages: P.V. Borges, et al.; Mol. Pharmacol. 88, 949 (2015), Application(s): Western Blot, Abstract; Full Text
MAPK pathway activation by chronic lead-exposure increases vascular reactivity through oxidative stress/cyclooxygenase-2-dependent pathways: M.R. Simões, et al.; Toxicol. Appl. Pharmacol. 283, 127 (2015), Application(s): Western Blotting, Abstract;
SERCA-2a is Involved in the Right Ventricular Function Following Myocardial Infarction in Rats: A.A. Fernandes, et al.; Life Sci. 124, 24 (2015), Application(s): Western Blotting, Abstract;
p38 MAPK contributes to angiotensin II-induced COX-2 expression in aortic fibroblasts from normotensive and hypertensive rats: M. Salaices, et al. ; J. Hypertens. 27, 142 (2009), Application(s): WB using rat cell lysates, Abstract;
Efficient antitumor immunity in a murine colorectal cancer model induced by CEA RNA-electroporated B cells: T. Kim, et al. ; Eur. J. Immunol. 38, 2106 (2008), Application(s): EIA using mouse samples, Abstract;
Carbohydrate profiling and protein identification of tegumental and excreted/secreted glycoproteins of adult Schistosoma bovis worms: R. Perez-Sanchez, et al. ; Vet. Parasitol. 144, 45 (2007), Application(s): WB using mouse samples, Abstract;
Polyreactive myelin oligodendrocyte glycoprotein antibodies:Implications for systemic autoimmunity in progressive experimental autoimmune encephalomyelitis: R. Fujinami, et al. ; J. Neuroimmunol. 183, 69 (2007), Application(s): EIA using mouse serum, Abstract;

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