ELISA (1:2,500-1:25,000) Western Blot (1:2500-1:25,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:
Immunoaffinity purified.
Quality Control:
The goat antiserum was immunoaffinity purified to isolate anti-mouse IgG specific antibodies which were then adsorbed with human IgG and conjugated to alkaline phosphatise (AP).
Formulation:
Liquid. In 0.005M TRIS-hydrogen chloride, pH 8.0, containing 0.125M sodium chloride, 7.5mg/mL BSA, 50% glycerol and 0.025% sodium azide.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Scientific Background:
Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
Product Literature References
Thermal Preference Ranges Correlate with Stable Signals of Universal Stress Markers in Lake Baikal Endemic and Holarctic Amphipods: D. Axenov-Gribanov, et al.; PLoS One 11, e0164226 (2016), Application(s): Western blot to determine the level of Hsp70, Abstract; Full Text
Prion protein-deficient mice exhibit decreased CD4 T and LTi cell numbers and impaired spleen structure: S. Kim, et al. ; Immunobiology 221, 94 (2015), Application(s): Western blot , Abstract;
Developmental, metabolic and immunological costs of flea infestation in the common vole: G. Devevey, et al. ; Funct. Ecol. 22, 1091 (2008), Application(s): ELISA using mouse serum, Abstract;
Identification and biochemical characterization of plant acylamino acid-releasing enzyme: K. Tanaka, et al. ; J. Biochem. 134, 251 (2003), Application(s): WB using plant (arabidopsis) samples, Abstract;