ELISA (1:2,500-1:25,000) Western Blot (1:2500-1:25,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Purity Detail:
Immunoaffinity purified.
Quality Control:
The goat antiserum was immunoaffinity purified to isolate anti-mouse IgG specific antibodies which were then adsorbed with human IgG and conjugated to alkaline phosphatise (AP).
Formulation:
Liquid. In 0.005M TRIS-hydrogen chloride, pH 8.0, containing 0.125M sodium chloride, 7.5mg/mL BSA, 50% glycerol and 0.025% sodium azide.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Thermal Preference Ranges Correlate with Stable Signals of Universal Stress Markers in Lake Baikal Endemic and Holarctic Amphipods: D. Axenov-Gribanov, et al.; PLoS One 11, e0164226 (2016), Application(s): Western blot to determine the level of Hsp70, Abstract; Full Text
Prion protein-deficient mice exhibit decreased CD4 T and LTi cell numbers and impaired spleen structure: S. Kim, et al. ; Immunobiology 221, 94 (2015), Application(s): Western blot , Abstract;
Developmental, metabolic and immunological costs of flea infestation in the common vole: G. Devevey, et al. ; Funct. Ecol. 22, 1091 (2008), Application(s): ELISA using mouse serum, Abstract;
Identification and biochemical characterization of plant acylamino acid-releasing enzyme: K. Tanaka, et al. ; J. Biochem. 134, 251 (2003), Application(s): WB using plant (arabidopsis) samples, Abstract;