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Goat anti-mouse IgG F(ab')2, polyclonal antibody (HRP conjugate)

 
ADI-SAB-100-J 1 ml 118.00 USD
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Product Specification

Host:Goat
 
Immunogen:Fab fragment of mouse IgG.
 
Species reactivity:Mouse
 
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab’)2/Fab portion of mouse IgG, and also with the light chains of other mouse immunoglobulins. No antibody was detected against the Fc portion of mouse IgG or against other non-immunoglobulin serum proteins.
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (1:2,500-1:50,000)
Immunohistochemistry (1:250-1:2500)
Western Blot (1:2500-1:50,000 colorimetric, 1:5,000-1:100,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Quality Control:The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins.
 
Formulation:Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
 

Product Literature References

Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One 12, e0183028 (2017), Application(s): Western Blot, Abstract; Full Text
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep. 6, 29389 (2016), Application(s): Antibody - immunofluorescence assay, Abstract; Full Text
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed. 11, 2407 (2016), Application(s): Western blot, Abstract; Full Text
Simvastatin induces differentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways: Y. Han, et al.; Exp. Cell Res. 346, 198 (2016), Application(s): Immunohistochemistry, Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res. 48, 813 (2015), Application(s): Western Blot, Abstract; Full Text
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl). 128, 1465 (2015), Application(s): Western Blot, Abstract; Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med. (2015), Application(s): Western Blot, Full Text
Heme Oxygenase-1 Protects Endothelial Cells from the Toxicity of Air Pollutant Chemicals: A. Lawal, et al.; Toxicol. Appl. Pharmacol. 284, 281 (2015), Application(s): Western Blotting, Abstract;
Molecular Characterization and expression analysis of hsp60 gene homologue of Sheep Blowfly, Lucilia cuprina: M.K. Singh, et al.; J. Therm. Biol. 52, 24 (2015), Application(s): Western Blot, Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
Low mercury concentrations cause oxidative stress and endothelial dysfunction in conductance and resistance arteries: M. Salaices, et al. ; Am. J. Physiol. Heart Circ. Physiol. 295, H1033 (2008), Application(s): WB using rat tissue, Abstract;
Persistent sub-lethal chlorine exposure augments temperature induced immunosuppression in Cyprinus carpio advanced fingerlings: A. Pal, et al. ; Fish Shellfish Immunol. 22, 547 (2007), Application(s): WB using fish samples, Abstract;
Oxidative stress and induction of heme oxygenase-1 in the kidney in sickle cell disease: R.P. Hebbel, et al. ; Am. J. Pathol. 158, 893 (2001), Application(s): IHC using human samples, Abstract;
Zinc metallothionein imported into liver mitochondria modulates respiration: B.L. Vallee, et al. ; PNAS 98, 2317 (2001), Application(s): WB using rat samples, Abstract;

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