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Goat anti-mouse IgG F(ab')2, polyclonal antibody (HRP conjugate)

 
ADI-SAB-100-J 1 ml 124.00 USD
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Product Specification

Host:Goat
 
Immunogen:Fab fragment of mouse IgG.
 
Species reactivity:Mouse
 
Specificity:Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab’)2/Fab portion of mouse IgG, and also with the light chains of other mouse immunoglobulins. No antibody was detected against the Fc portion of mouse IgG or against other non-immunoglobulin serum proteins.
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (1:2,500-1:50,000)
Immunohistochemistry (1:250-1:2500)
Western Blot (1:2500-1:50,000 colorimetric, 1:5,000-1:100,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Quality Control:The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins.
 
Formulation:Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. Assay Designs offers isotyping kits and antibodies in a variety of formats for the detection of human, mouse, rabbit, rat, sheep, and goat IgG.
 

Product Literature References

AGR2 is a target of canonical Wnt/β-catenin signaling and is important for stemness maintenance in colorectal cancer stem cells: B. Dahal Lamichane, et al.; Biochem. Biophys. Res. Commun. 515, 600 (2019), Abstract;
Rap1 regulates hepatic stellate cell migration through the modulation of RhoA activity in response to TGF-β2: M.Y. Moon, et al.; Int. J. Mol. Med. 44, 491 (2019), Abstract; Full Text
The activation of retinal HCA2 receptors by systemic beta-hydroxybutyrate inhibits diabetic retinal damage through reduction of endoplasmic reticulum stress and the NLRP3 inflammasome: M.C. Trotta, et al.; PLoS One 14, e0211005 (2019), Abstract; Full Text
Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse: S. Wang, et al.; Biomaterials 219, 119375 (2019), Abstract;
An Aqueous Extract of Herbal Medicine ALWPs Enhances Cognitive Performance and Inhibits LPS-Induced Neuroinflammation via FAK/NF-κB Signaling Pathways: J.Y. Lee, et al.; Front. Aging Neurosci. 10, 269 (2018), Abstract; Full Text
Combined therapy involving electroacupuncture and treadmill exercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp. Neurol. 300, 222 (2018), Abstract;
Combined therapy involving electroacupuncture and treadmillexercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia: M.E. Pak, et al.; Exp.Neurol. 300, 222 (2018), Application(s): Western Blot, Abstract;
Identification and characterization of site-specific N-glycosylation in the potassium channel Kv3.1b: P.C. Vicente, et al.; J. Cell. Physiol. 233, 549 (2018), Abstract;
Osteogenic potential of cell spheroids composed of varying ratios of gingiva-derived and bone marrow stem cells using concave microwells: J.Y. Tae, et al.; Exp. Ther. Med. 16, 2287 (2018), Abstract; Full Text
PEP-1-glutaredoxin-1 induces dedifferentiation of rabbit articular chondrocytes by the endoplasmic reticulum stress-dependent ERK-1/2 pathway and the endoplasmic reticulum stress-independent p38 kinase and PI-3 kinase pathways: S.M. Yu, et al.; Int. J. Biol. Macromol. 111, 1059 (2018), Abstract;
Simvastatin-dependent actin cytoskeleton rearrangement regulates differentiation via the extracellular signal-regulated kinase-1/2 and p38 kinase pathways in rabbit articular chondrocytes: Y. Han, et al.; Eur. J. Pharmacol. 834, 197 (2018), Abstract;
Analysis of endogenous lipids during intestinal wound healing: Y. Lee, et al.; PLoS One 12, e0183028 (2017), Application(s): Western Blot, Abstract; Full Text
cPKCγ membrane translocation is involved in herkinorin‑induced neuroprotection against cerebral ischemia/reperfusion injury in mice: X. Gui, et al.; Mol. Med. Rep. 15, 221 (2017), Abstract; Full Text
Fluid shear stress regulates vascular remodeling via VEGFR-3 activation, although independently of its ligand, VEGF-C, in the uterus during pregnancy: Y.G. Park, et al.; Int. J. Mol. Med. 40, 1210 (2017), Abstract; Full Text
Korean Scutellaria baicalensis Georgi flavonoid extract induces mitochondrially mediated apoptosis in human gastric cancer AGS cells: V. Ventatarame Gowda Saralamma, et al.; Oncol. Lett. 14, 607 (2017), Abstract; Full Text
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array: S. Yumnam, et al.; Mol. Med. Rep. 16, 5386 (2017), Abstract; Full Text
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones 22, 225 (2017), Application(s): Western Blot, Abstract; Full Text
Berberine induces dedifferentiation by actin cytoskeleton reorganization via phosphoinositide 3-kinase/Akt and p38 kinase pathways in rabbit articular chondrocytes: S.M. Yu, et al.; Exp. Biol. Med. 241, 800 (2016), Abstract; Full Text
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1: H.S. Seol, et al.; Cancer Lett. 372, 24 (2016), Abstract; Full Text
Cytokine-induced apoptosis inhibitor-1 causes dedifferentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways: S.M. Yu, et al.; Int. J. Biochem. Cell Biol. 80, 10 (2016), Abstract;
Erythropoietin and a nonerythropoietic peptide analog promote aortic endothelial cell repair under hypoxic conditions: role of nitric oxide: L. Heikal, et al.; Hypoxia 4, 121 (2016), Abstract; Full Text
Hypoxia‑mediated activation of autophagic flux inhibits apoptosis of keratinocytes via blocking tumor necrosis factor‑related apoptosis‑inducing ligand: S.W. Kim, et al.; Mol. Med. Rep. 13, 805 (2016), Abstract;
Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression: H. Nassour, et al.; Sci. Rep. 6, 29389 (2016), Application(s): Antibody - immunofluorescence assay, Abstract; Full Text
Proliferation of Prostate Stromal Cell Induced by Benign Prostatic Hyperplasia Epithelial Cell Stimulated With Trichomonas vaginalis via Crosstalk With Mast Cell: J.H. Kim, et al.; Prostate 76, 1431 (2016), Abstract;
Schisandrae fructus enhances myogenic differentiation and inhibits atrophy through protein synthesis in human myotubes: C.H. Kim, et al.; Int. J. Nanomed. 11, 2407 (2016), Application(s): Western blot, Abstract; Full Text
Simvastatin induces differentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways: Y. Han, et al.; Exp. Cell Res. 346, 198 (2016), Application(s): Immunohistochemistry, Abstract;
A single resistance exercise session improves myocardial contractility in spontaneously hypertensive rats: A.A. Fernandes, et al.; Braz. J. Med. Biol. Res. 48, 813 (2015), Application(s): Western Blot, Abstract; Full Text
Correlation of Lower Concentrations of Hydrogen Sulfide with Activation of Protein Kinase CβII in Uremic Accelerated Atherosclerosis Patients: W. Wang, et al.; Chin. Med. J. (Engl). 128, 1465 (2015), Application(s): Western Blot, Abstract; Full Text
Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway: G. Dong, et al.; J. Funct. Foods 18, 47 (2015), Application(s): Immunoprecipitation,
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med. 10, 711 (2015), Application(s): Western Blot, Abstract; Full Text
Heme Oxygenase-1 Protects Endothelial Cells from the Toxicity of Air Pollutant Chemicals: A. Lawal, et al.; Toxicol. Appl. Pharmacol. 284, 281 (2015), Application(s): Western Blotting, Abstract;
Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest: S.J. Park, et al.; Am. J. Cancer Res. 5, 2127 (2015), Abstract; Full Text
Molecular Characterization and expression analysis of hsp60 gene homologue of Sheep Blowfly, Lucilia cuprina: M.K. Singh, et al.; J. Therm. Biol. 52, 24 (2015), Application(s): Western Blot, Abstract;
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
[6]-Gingerol Affects Glucose Metabolism by Dual Regulation via the AMPKα2-Mediated AS160-Rab5 Pathway and AMPK-Mediated Insulin Sensitizing Effects: J.O. Lee, et al.; J. Cell. Biochem. 116, 1401 (2015), Abstract;
Src regulates membrane trafficking of the Kv3.1b channel: S.H. Bae, et al.; FEBS Lett. 588, 86 (2014), Abstract;
Low mercury concentrations cause oxidative stress and endothelial dysfunction in conductance and resistance arteries: M. Salaices, et al. ; Am. J. Physiol. Heart Circ. Physiol. 295, H1033 (2008), Application(s): WB using rat tissue, Abstract;
Persistent sub-lethal chlorine exposure augments temperature induced immunosuppression in Cyprinus carpio advanced fingerlings: A. Pal, et al. ; Fish Shellfish Immunol. 22, 547 (2007), Application(s): WB using fish samples, Abstract;
Oxidative stress and induction of heme oxygenase-1 in the kidney in sickle cell disease: R.P. Hebbel, et al. ; Am. J. Pathol. 158, 893 (2001), Application(s): IHC using human samples, Abstract;
Zinc metallothionein imported into liver mitochondria modulates respiration: B.L. Vallee, et al. ; PNAS 98, 2317 (2001), Application(s): WB using rat samples, Abstract;

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