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HIF-1α monoclonal antibody (Hα111a)

ADI-OSA-602-E 100 µg 296.00 USD
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Product Specification

Alternative Name:Hypoxia-inducible factor 1α, ARNT-interacting protein
Immunogen:Recombinant human HIF-1α.
UniProt ID:Q16665
Species reactivity:Human
Applications:IHC, WB
Recommended Dilutions/Conditions:Western Blot (4µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~120kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex which regulates systemic, local and intracellular homeostatic responses elicited by hypoxia. HIF-1 is a heterodimer of HIF-1alpha and HIF-1beta. HIF-1beta is constitutively expressed and serves as a dimerization partner for several other transcription factors, while HIF-1alpha and the closely related HIF-2 are unique to HIF-1 and their expression is tightly regulated by cellular oxygen concentration. HIF-1 regulates a diverse group of genes, including erythropoietin, VEGF, glucose transporters, heme oxygenase, and NOS.
Technical Info/Product Notes:Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000100644).
HIF-1α monoclonal antibody (Hα111a) Western blot
Western blot analysis of HIF-1α, mAb (Hα111a) (Prod. No. ADI-OSA-602): Lane 1: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 2: HeLa+CoCl2 Cell Lysate.
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HIF-1α monoclonal antibody (Hα111a) Western blot

Product Literature References

Metabolic characteristics distinguishing intrahepatic cholangiocarcinoma: a negative pilot study of 18F-fluorocholine PET/CT clarified by transcriptomic analysis: S.A. Kwee, et al.; Am. J. Nucl. Med. Mol. Imaging 6, 73 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Physiologic hypoxia promotes maintenance of CML stem cells despite effective BCR-ABL1 inhibition: K.P. Ng, et al.; Blood 123, 3316 (2014), Application(s): Immunohistochemistry, Abstract;

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