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HO-1 monoclonal antibody (HO-1-2) (FITC conjugate)

 
ADI-OSA-111FI-D 50 µg 205.00 USD
 
ADI-OSA-111FI-F 200 µg 410.00 USD
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Product Specification

Alternative Name:HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1
 
Clone:HO-1-2
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Native rat HO-1.
 
UniProt ID:P06762
 
GenBank ID:J02722
 
Species reactivity:Human, Mouse, Rat
Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit
 
Applications:Flow Cytometry
 
Recommended Dilutions/Conditions:Flow Cytometry (1:20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~32kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
 
ADI-OSA-111FI FC
Flow cytometry analysis of 106 Jurkat cells were stained using HO-1 mAb (HO-1-2), Fluorescein Conjugate at a concentration of 50µg/ml.
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ADI-OSA-111FI FC

Product Literature References

α-Lipoic acid enhances endogenous peroxisome-proliferator-activated receptor-γ to ameliorate experimental autoimmune encephalomyelitis in mice: K.C. Wang, et al.; Clin. Sci. (Lond.) 125, 329 (2013), Abstract;
Heme oxygenase-1 induction alters chemokine regulation and ameliorates human immunodeficiency virus-type-1 infection in lipopolysaccharide-stimulated macrophages: Z.H. Zhou, et al.; BBRC 435, 373 (2013), Application(s): Flow cytometry of primary human monocyte-derived macrophages, Abstract;

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